SRR3553176_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553176_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	675661
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCT	2779	0.4113009334562746	Illumina PCR Primer Index 10 (95% over 24bp)
AATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCT	1047	0.1549593657174234	Illumina PCR Primer Index 10 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	930	0.13764298960573423	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCC	859	0.12713476136701687	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGT	841	0.1244707035036801	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAACG	25	3.8890917E-5	45.000004	1
CGTCTAG	25	3.8890917E-5	45.000004	1
TTATCCG	25	3.8890917E-5	45.000004	1
TCGCGAT	25	3.8890917E-5	45.000004	21
GACTATC	35	1.2109376E-7	45.000004	9
CCTTACG	20	7.03122E-4	45.0	1
TTTCGTA	20	7.03122E-4	45.0	27
ACTCGAC	20	7.03122E-4	45.0	34
CCGACGA	20	7.03122E-4	45.0	14
CGCGTGA	20	7.03122E-4	45.0	41
CGCGTAG	20	7.03122E-4	45.0	1
CGTTTCA	20	7.03122E-4	45.0	37
ACCCGTA	30	2.164168E-6	44.999996	34
TCGACGG	30	2.164168E-6	44.999996	2
CTTACGG	90	0.0	42.500004	2
CATACGG	80	0.0	42.1875	2
TACGGGT	70	0.0	41.785717	4
GGCCGAT	130	0.0	41.538464	8
GCCGATG	125	0.0	41.399998	9
ACCGATC	60	3.6379788E-12	41.249996	25