##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553176_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	675661
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.81514990505594	33.0	31.0	34.0	30.0	34.0
2	32.022324804894765	33.0	31.0	34.0	30.0	34.0
3	32.19550632639741	34.0	31.0	34.0	30.0	34.0
4	35.84665090925775	37.0	35.0	37.0	35.0	37.0
5	35.60054672387484	37.0	35.0	37.0	33.0	37.0
6	34.959432318869965	37.0	35.0	37.0	33.0	37.0
7	35.4023541391319	37.0	35.0	37.0	33.0	37.0
8	35.80292335949537	37.0	35.0	37.0	35.0	37.0
9	37.47078786551244	39.0	37.0	39.0	35.0	39.0
10	36.992601319300654	39.0	37.0	39.0	33.0	39.0
11	36.971610023369706	39.0	37.0	39.0	33.0	39.0
12	36.85982911548839	39.0	37.0	39.0	33.0	39.0
13	36.84157735906024	39.0	37.0	39.0	33.0	39.0
14	38.034686033380645	40.0	37.0	41.0	33.0	41.0
15	38.03968706200299	40.0	37.0	41.0	33.0	41.0
16	37.95106865721124	40.0	37.0	41.0	33.0	41.0
17	37.9571989503612	40.0	37.0	41.0	33.0	41.0
18	37.936650184041994	40.0	37.0	41.0	33.0	41.0
19	37.980950506244994	40.0	37.0	41.0	33.0	41.0
20	37.88250912809826	40.0	37.0	41.0	33.0	41.0
21	37.8787957274432	40.0	37.0	41.0	33.0	41.0
22	37.859975638670875	40.0	37.0	41.0	33.0	41.0
23	37.769508673728396	40.0	37.0	41.0	33.0	41.0
24	37.61495927691549	39.0	36.0	41.0	32.0	41.0
25	37.639659533405066	39.0	36.0	41.0	33.0	41.0
26	37.585037467013784	39.0	36.0	41.0	33.0	41.0
27	37.48299369062296	39.0	36.0	41.0	32.0	41.0
28	37.448745746757616	39.0	36.0	41.0	32.0	41.0
29	37.280682176416875	39.0	36.0	41.0	32.0	41.0
30	37.30180667524099	39.0	36.0	41.0	32.0	41.0
31	37.296081910899105	39.0	36.0	41.0	32.0	41.0
32	37.27307037108846	39.0	36.0	41.0	32.0	41.0
33	37.05784409637377	39.0	35.0	41.0	31.0	41.0
34	37.05074142210369	39.0	35.0	41.0	31.0	41.0
35	36.9874848481709	39.0	35.0	41.0	31.0	41.0
36	36.96004209211424	39.0	35.0	41.0	31.0	41.0
37	36.87564029890729	39.0	35.0	41.0	31.0	41.0
38	36.69578087236055	39.0	35.0	41.0	31.0	41.0
39	36.668307035628814	39.0	35.0	40.0	31.0	41.0
40	36.5606820580143	39.0	35.0	40.0	30.0	41.0
41	36.53245340488795	39.0	35.0	40.0	30.0	41.0
42	36.46641881061657	39.0	35.0	40.0	30.0	41.0
43	36.40286327019023	39.0	35.0	40.0	30.0	41.0
44	36.296932929383225	38.0	35.0	40.0	30.0	41.0
45	36.09212608097848	38.0	35.0	40.0	30.0	41.0
46	35.94617715096772	38.0	35.0	40.0	30.0	41.0
47	35.811563787165454	38.0	35.0	40.0	29.0	41.0
48	35.7162896778118	38.0	34.0	40.0	29.0	41.0
49	35.604758895363204	38.0	34.0	40.0	28.0	41.0
50	35.52867488281846	38.0	34.0	40.0	28.0	41.0
51	34.25098681143354	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	6.0
12	5.0
13	15.0
14	25.0
15	46.0
16	74.0
17	125.0
18	245.0
19	421.0
20	759.0
21	1095.0
22	1653.0
23	2188.0
24	2768.0
25	3479.0
26	4433.0
27	5437.0
28	7006.0
29	8857.0
30	11697.0
31	15453.0
32	20445.0
33	27805.0
34	43135.0
35	53377.0
36	63748.0
37	93377.0
38	140233.0
39	167710.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.09513646636405	32.09390507961833	20.914482262554742	26.896476191462877
2	22.50033670731328	30.013127885137663	20.763370980417694	26.72316442713136
3	20.91566628827178	30.511454708796276	21.262585823364084	27.310293179567864
4	21.450993915587844	30.073069187062746	19.730752551945425	28.745184345403985
5	19.527248131829424	33.02810137036176	18.020871413327097	29.423779084481716
6	20.737766424286736	37.812749292914646	18.652549133367177	22.796935149431448
7	85.8020516205612	5.0810983614564105	5.028409217048194	4.088440800934197
8	87.34824120379895	3.973442303166825	4.021543347921517	4.6567731451127115
9	79.7607084025865	7.774608864504537	7.821377880327561	4.643304852581398
10	36.010810154796566	34.011138721933044	13.287580606250767	16.69047051701963
11	26.85148321421541	29.15678128528952	24.94446179371016	19.04727370678491
12	26.96870176020223	24.324920337269727	28.88874746359491	19.817630438933133
13	25.538102687590374	24.816143006626103	27.401463159779833	22.244291146003693
14	22.430923199651897	27.159033894216183	27.90867017631623	22.501372729815692
15	20.607079585768602	28.81652189485556	28.67044272201592	21.90595579735992
16	23.154954925620984	27.010882676371732	27.664908881820914	22.169253516186373
17	23.24819695083777	27.311477205284902	26.892776111097135	22.547549732780194
18	23.720771215150794	26.425529962510787	26.555772791385028	23.29792603095339
19	23.31746245528453	29.35406957039107	25.31580185921638	22.012666115108022
20	25.538250690805004	27.514833622186273	25.264740750169096	21.68217493683963
21	24.559209426028733	27.7482346916575	25.632972748168093	22.059583134145676
22	23.285197754495226	25.389951469745924	25.55749110870688	25.76735966705197
23	22.638127700133648	27.644188431772736	25.36257087503941	24.35511299305421
24	21.52543953254665	28.360671993795705	26.257842320335197	23.85604615332245
25	23.560927743350586	27.943154925324976	24.09891942852999	24.39699790279445
26	24.61234258008084	27.565450721589674	23.76502417632511	24.05718252200438
27	21.47423042028473	27.22045522828756	25.65724527536738	25.64806907606033
28	21.868806990487833	27.1581458749284	26.864359493888205	24.108687640695557
29	22.55095380671668	27.505805426093854	24.649787393382184	25.293453373807278
30	22.859540509219862	28.230281161706834	24.633211033343645	24.276967295729662
31	23.581204183754874	27.201214810385682	23.9833289179041	25.23425208795535
32	23.558855698345766	27.13416935415837	24.34638080339105	24.96059414410481
33	22.58588256536932	27.340633838566973	24.939133677983484	25.134349918080222
34	21.82869811932315	26.22232154882404	26.377576920970725	25.57140341088208
35	21.833434222191308	26.293955104704875	25.877770065165816	25.994840607938002
36	23.48263404281141	28.317899064767687	25.680629783278892	22.51883710914201
37	22.172065577264338	29.383966219746295	24.532124837751475	23.911843365237893
38	23.638037418172722	27.053063592541232	27.14556560168487	22.163333387601178
39	22.675128503791104	25.789560149246444	26.782809722627178	24.75250162433528
40	23.711891022273004	26.158088153674697	25.828336991479457	24.30168383257284
41	22.275815830719843	24.444358931475993	27.029383078200457	26.250442159603704
42	22.878928930336368	25.860009679410233	25.328234129245285	25.932827261008107
43	21.78207710671476	25.148854233113944	26.875163728556185	26.193904931615116
44	23.13260644021188	23.8131252210798	26.80501020482165	26.249258133886666
45	23.69176258508335	24.541893049917043	27.487009017835867	24.27933534716374
46	21.403188877262412	25.818420776099256	28.28267429968579	24.49571604695254
47	22.60142290290545	26.40599353817965	26.08541857529146	24.907164983623446
48	22.771774602944376	24.96769829840704	28.464570250465844	23.79595684818274
49	22.163037381171918	25.24920041263296	27.89431386449714	24.693448341697984
50	21.973445263231117	24.3977379188676	27.36594238826867	26.26287442963261
51	21.58094073803283	24.441398867183395	28.59732913398879	25.380331260794986
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1223.0
1	1023.5
2	824.0
3	568.0
4	312.0
5	316.5
6	321.0
7	421.0
8	521.0
9	559.0
10	597.0
11	701.0
12	805.0
13	851.0
14	897.0
15	945.5
16	994.0
17	1010.5
18	1027.0
19	1198.0
20	1369.0
21	1533.0
22	1697.0
23	2379.5
24	3062.0
25	3764.5
26	5416.5
27	6366.0
28	6952.0
29	7538.0
30	9401.0
31	11264.0
32	13692.5
33	16121.0
34	16466.5
35	16812.0
36	18158.5
37	19505.0
38	21144.0
39	22783.0
40	25228.5
41	27674.0
42	30097.5
43	32521.0
44	37932.0
45	43343.0
46	48588.0
47	53833.0
48	57652.5
49	61472.0
50	59920.5
51	58369.0
52	53802.0
53	49235.0
54	45155.5
55	41076.0
56	38797.0
57	36518.0
58	35236.0
59	33954.0
60	31736.0
61	29518.0
62	27060.0
63	24602.0
64	21452.0
65	18302.0
66	15794.0
67	13286.0
68	11976.0
69	10666.0
70	9124.0
71	7582.0
72	6680.0
73	5778.0
74	4711.0
75	3306.5
76	2969.0
77	2201.0
78	1433.0
79	1033.5
80	634.0
81	464.0
82	294.0
83	226.0
84	158.0
85	142.5
86	127.0
87	116.0
88	105.0
89	76.5
90	48.0
91	27.0
92	6.0
93	5.0
94	4.0
95	3.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	675661.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.692982690883287
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.98945160792121	21.043745680678754
2	9.523635195568275	5.274757292503183
3	3.2598869222786626	2.708279763386994
4	1.613977313731614	1.7878338325059158
5	0.9881811238747857	1.368284137946102
6	0.6853820555956468	1.1388164041351352
7	0.4984960434577553	0.9663389612064595
8	0.4642545106151001	1.0285273701302766
9	0.3464487776358072	0.8634780002111476
>10	5.2929064837601505	36.105515127497185
>50	1.2344207747296503	22.420155421278036
>100	0.09860112984380633	4.0531457965728706
>500	0.0032685457406786632	0.6639332508548481
>1k	0.0010895152468928877	0.5771889610930808
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCT	2779	0.4113009334562746	Illumina PCR Primer Index 10 (95% over 24bp)
AATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCT	1047	0.1549593657174234	Illumina PCR Primer Index 10 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	930	0.13764298960573423	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCC	859	0.12713476136701687	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGT	841	0.1244707035036801	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9600642925964354E-4	0.0	0.0	0.47183424823987175	0.0
2	2.9600642925964354E-4	0.0	0.0	0.711599455940183	0.0
3	2.9600642925964354E-4	0.0	0.0	0.9987256923220372	0.0
4	2.9600642925964354E-4	0.0	0.0	2.180383357926534	0.0
5	2.9600642925964354E-4	0.0	0.0	2.37515558837938	0.0
6	2.9600642925964354E-4	0.0	0.0	3.377877367496422	0.0
7	2.9600642925964354E-4	0.0	0.0	4.13491381032796	0.0
8	2.9600642925964354E-4	0.0	0.0	4.832008951234421	0.0
9	2.9600642925964354E-4	0.0	0.0	5.731276483325217	0.0
10	2.9600642925964354E-4	0.0	0.0	7.053241196398786	0.0
11	2.9600642925964354E-4	0.0	0.0	8.180285675804878	0.0
12	2.9600642925964354E-4	0.0	0.0	8.719609389915949	0.0
13	2.9600642925964354E-4	0.0	0.0	8.962482665123487	0.0
14	2.9600642925964354E-4	0.0	0.0	9.23273653503754	0.0
15	2.9600642925964354E-4	0.0	0.0	9.477681855249896	0.0
16	2.9600642925964354E-4	0.0	0.0	9.910295251612865	0.0
17	2.9600642925964354E-4	0.0	0.0	10.441774795348556	0.0
18	2.9600642925964354E-4	0.0	0.0	11.188599016370636	0.0
19	2.9600642925964354E-4	0.0	0.0	11.497925734946962	0.0
20	2.9600642925964354E-4	0.0	0.0	11.893686330867107	0.0
21	2.9600642925964354E-4	0.0	0.0	12.308687344689126	0.0
22	2.9600642925964354E-4	0.0	0.0	12.738636683188759	0.0
23	2.9600642925964354E-4	0.0	0.0	13.181314298146555	0.0
24	2.9600642925964354E-4	0.0	0.0	13.515949566424583	0.0
25	2.9600642925964354E-4	0.0	0.0	13.803223806021066	0.0
26	2.9600642925964354E-4	0.0	0.0	14.078065775588646	0.0
27	2.9600642925964354E-4	0.0	0.0	14.378808307716444	0.0
28	2.9600642925964354E-4	0.0	0.0	14.694351161307223	0.0
29	2.9600642925964354E-4	0.0	0.0	15.018478201346534	0.0
30	2.9600642925964354E-4	0.0	0.0	15.441323385543933	0.0
31	2.9600642925964354E-4	0.0	0.0	15.730965676574495	0.0
32	2.9600642925964354E-4	0.0	0.0	16.046360526950647	0.0
33	2.9600642925964354E-4	0.0	0.0	16.351987165161226	0.0
34	2.9600642925964354E-4	0.0	0.0	16.653617716576804	0.0
35	2.9600642925964354E-4	0.0	0.0	16.987956978425572	0.0
36	2.9600642925964354E-4	0.0	0.0	17.309123954172286	0.0
37	2.9600642925964354E-4	0.0	0.0	17.61282655059268	0.0
38	2.9600642925964354E-4	0.0	0.0	17.922301272383635	0.0
39	2.9600642925964354E-4	0.0	0.0	18.240804190267013	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAACG	25	3.8890917E-5	45.000004	1
CGTCTAG	25	3.8890917E-5	45.000004	1
TTATCCG	25	3.8890917E-5	45.000004	1
TCGCGAT	25	3.8890917E-5	45.000004	21
GACTATC	35	1.2109376E-7	45.000004	9
CCTTACG	20	7.03122E-4	45.0	1
TTTCGTA	20	7.03122E-4	45.0	27
ACTCGAC	20	7.03122E-4	45.0	34
CCGACGA	20	7.03122E-4	45.0	14
CGCGTGA	20	7.03122E-4	45.0	41
CGCGTAG	20	7.03122E-4	45.0	1
CGTTTCA	20	7.03122E-4	45.0	37
ACCCGTA	30	2.164168E-6	44.999996	34
TCGACGG	30	2.164168E-6	44.999996	2
CTTACGG	90	0.0	42.500004	2
CATACGG	80	0.0	42.1875	2
TACGGGT	70	0.0	41.785717	4
GGCCGAT	130	0.0	41.538464	8
GCCGATG	125	0.0	41.399998	9
ACCGATC	60	3.6379788E-12	41.249996	25
>>END_MODULE