FastQCFastQC Report
Sat 18 Jun 2016
SRR3553174_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553174_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173482
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8800.5072572370620583No Hit
CTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGCT7950.4582607993912913Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC2400.13834288283510682No Hit
AAACTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCT2380.1371900254781476No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2290.13200216737183107No Hit
AAAAACTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTT2100.12105002248071846No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAA207.016713E-445.00000437
TCTGCGG207.016713E-445.0000042
GGTACCT302.1548185E-645.0000048
TACCCGG207.016713E-445.0000042
GATAATA253.877074E-545.00000440
CACGGGT253.877074E-545.0000044
CTATCGG207.016713E-445.0000042
CTATATA207.016713E-445.00000425
GGGTACC302.1548185E-645.0000047
GGCAACG302.1548185E-645.0000041
AAGACTA207.016713E-445.00000416
ATAGCGG253.877074E-545.0000042
CCAATAG207.016713E-445.0000041
CCTACGA207.016713E-445.00000432
TTGTAAG207.016713E-445.0000041
CGATTGA207.016713E-445.00000415
AAGCGAC253.877074E-545.00000414
TGCGGGT253.877074E-545.0000044
TGACGAC207.016713E-445.00000422
ACGGGTC207.016713E-445.0000045