##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553174_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	173482
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.320217659468994	33.0	31.0	34.0	31.0	34.0
2	32.51981185367934	34.0	31.0	34.0	31.0	34.0
3	32.66921063856769	34.0	31.0	34.0	31.0	34.0
4	36.241552437716884	37.0	37.0	37.0	35.0	37.0
5	36.03553106374148	37.0	35.0	37.0	35.0	37.0
6	35.219290762153996	37.0	35.0	37.0	35.0	37.0
7	35.57311421357836	37.0	35.0	37.0	35.0	37.0
8	36.002616986200294	37.0	35.0	37.0	35.0	37.0
9	37.708805524492455	39.0	38.0	39.0	35.0	39.0
10	37.47749046010537	39.0	37.0	39.0	35.0	39.0
11	37.47857414602091	39.0	37.0	39.0	35.0	39.0
12	37.35068191512664	39.0	37.0	39.0	35.0	39.0
13	37.32839718241662	39.0	37.0	39.0	35.0	39.0
14	38.6038090407074	40.0	38.0	41.0	35.0	41.0
15	38.647237177344046	40.0	38.0	41.0	35.0	41.0
16	38.548587173309045	40.0	38.0	41.0	35.0	41.0
17	38.53700095687161	40.0	38.0	41.0	35.0	41.0
18	38.48029190348278	40.0	38.0	41.0	34.0	41.0
19	38.46723579391522	40.0	38.0	41.0	34.0	41.0
20	38.364689132013694	40.0	37.0	41.0	34.0	41.0
21	38.34115354907137	40.0	37.0	41.0	34.0	41.0
22	38.32500778178716	40.0	37.0	41.0	34.0	41.0
23	38.22639812775965	40.0	37.0	41.0	34.0	41.0
24	38.08421622992587	40.0	37.0	41.0	34.0	41.0
25	38.06017338974649	40.0	37.0	41.0	34.0	41.0
26	38.07633068560427	40.0	37.0	41.0	34.0	41.0
27	37.993659284536726	40.0	37.0	41.0	34.0	41.0
28	37.927122122179824	40.0	37.0	41.0	34.0	41.0
29	37.68022042632665	40.0	36.0	41.0	33.0	41.0
30	37.730611821399336	40.0	36.0	41.0	33.0	41.0
31	37.63479208217567	40.0	36.0	41.0	33.0	41.0
32	37.572422499164176	40.0	36.0	41.0	33.0	41.0
33	37.38654154321486	40.0	36.0	41.0	33.0	41.0
34	37.28755721054634	40.0	36.0	41.0	33.0	41.0
35	37.21052328195432	40.0	35.0	41.0	33.0	41.0
36	37.165700187915746	40.0	35.0	41.0	33.0	41.0
37	37.03436091352417	39.0	35.0	41.0	32.0	41.0
38	36.896329302175445	39.0	35.0	41.0	32.0	41.0
39	36.84882005049515	39.0	35.0	41.0	32.0	41.0
40	36.75908739811623	39.0	35.0	41.0	31.0	41.0
41	36.6672334882005	39.0	35.0	41.0	31.0	41.0
42	36.587340473363234	39.0	35.0	41.0	31.0	41.0
43	36.54369905811554	39.0	35.0	41.0	31.0	41.0
44	36.44914746198453	39.0	35.0	41.0	31.0	41.0
45	36.24039381607314	38.0	35.0	40.0	31.0	41.0
46	36.089444438039685	38.0	35.0	40.0	31.0	41.0
47	36.009885751835924	38.0	35.0	40.0	30.0	41.0
48	35.864746774881546	38.0	35.0	40.0	30.0	41.0
49	35.72977023552876	38.0	35.0	40.0	30.0	41.0
50	35.673372453626314	38.0	35.0	40.0	30.0	41.0
51	34.4385065885798	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	3.0
13	2.0
14	2.0
15	3.0
16	16.0
17	16.0
18	49.0
19	113.0
20	225.0
21	378.0
22	509.0
23	673.0
24	868.0
25	913.0
26	993.0
27	1091.0
28	1239.0
29	1551.0
30	1994.0
31	2520.0
32	3558.0
33	5554.0
34	10183.0
35	13624.0
36	15152.0
37	23778.0
38	38941.0
39	49510.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.010721573419723	33.26108760563056	19.92137512825538	26.806815692694343
2	23.538465085714943	31.05336576705364	20.142147312113075	25.26602183511834
3	20.79351171879503	31.91455021270218	19.89024797961748	27.4016900888853
4	20.366954496720123	31.835003055071997	18.83653635535675	28.96150609285113
5	19.432563608904672	33.628849102500546	16.58212379382299	30.35646349477179
6	18.834807069321315	37.82352059579669	18.490679148268985	24.85099318661302
7	83.29913189841021	7.336784219688498	5.341764563470562	4.02231931843073
8	85.57833089311859	5.440910296169054	4.045952894248394	4.934805916463956
9	77.94929733344094	9.164639559147346	8.006017915403326	4.880045192008393
10	38.33250711889418	30.901765024613503	13.10510600523397	17.660621851258345
11	27.653589421380893	29.66417265191778	23.042159993543997	19.64007793315733
12	27.338282934252543	24.271105936062533	29.001279671666225	19.3893314580187
13	26.67769566871491	24.255542361743583	25.84879122906123	23.217970740480283
14	20.44361951095791	28.789153917985725	27.991376626969945	22.775849944086417
15	20.41422164835545	28.839879641691933	28.289966682422385	22.455932027530235
16	22.86289067453684	27.412065805097935	27.31637864447032	22.408664875894903
17	22.154459828685397	28.10608593398739	26.026331262032947	23.71312297529427
18	23.63127010295016	27.428782236773845	25.38822471495602	23.551722945319973
19	23.266967178151045	30.22561418475692	24.842346756435827	21.665071880656207
20	25.99405125603809	27.470285101624377	24.604858140902223	21.93080550143531
21	24.918435341995135	28.638129604224073	24.45037525506969	21.993059798711105
22	22.254181990062367	26.95899286381296	25.261986834368983	25.524838311755687
23	23.40992149041399	27.599981554282287	25.012393216587313	23.977703738716407
24	22.337187719763435	28.31071811484765	25.24872897476395	24.103365190624963
25	22.0553140959869	28.969576094349847	24.49476026331262	24.48034954635063
26	23.962140164397457	28.40121741736895	24.112011620802157	23.524630797431435
27	21.12726392363473	26.353166322730885	26.6327342317935	25.886835521840883
28	22.49109417691749	27.89050160823601	26.610253513332793	23.008150701513703
29	22.51703346744907	30.24002490171891	24.043993036741565	23.198948594090453
30	23.12747143795898	26.524365640239335	26.841977842081597	23.506185079720083
31	24.742048166380375	28.571263877520437	21.327861103745633	25.358826852353562
32	24.80545532101313	26.388328472118143	25.29138469697144	23.51483150989728
33	22.63865991860827	25.91277481237246	25.558847603785985	25.88971766523328
34	22.25072341799149	26.24767987456912	26.539352785879807	24.962243921559583
35	22.647306348785463	27.232796486090777	26.279383451885497	23.840513713238263
36	23.667008681015897	28.383924557014563	25.07234179914919	22.87672496282035
37	23.496385792185933	28.974187523777683	23.60533081241858	23.924095871617805
38	25.01354607394427	27.299662212794413	25.822851938529645	21.863939774731673
39	24.37832167025974	24.98530106869877	24.710921017742475	25.925456243299017
40	26.166979859581975	26.217705583288183	23.905073725227975	23.710240831901867
41	21.73539617943072	25.51965045364937	26.650027092147887	26.094926274772025
42	22.722242076987815	26.931900715924417	25.7110247749046	24.634832432183167
43	21.89621978072653	26.358930609515685	26.468452058426813	25.276397551330977
44	24.58237742244152	23.699865115689235	25.2671746924753	26.450582769393943
45	25.603232612028915	24.028429462422615	26.26093773417415	24.10740019137432
46	22.11872125061966	24.94379820384824	27.917593756124553	25.019886789407547
47	22.75625136901811	26.33529703369802	25.311559700718227	25.596891896565637
48	23.091156431214767	25.171487531847685	27.252395061159085	24.484960975778467
49	22.185586977323297	25.596891896565637	26.900773567286517	25.31674755882455
50	22.076641957090647	23.66009153687414	27.647248705917615	26.616017800117593
51	22.201726980320725	24.068779469916187	28.29342525449326	25.436068295269827
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1045.0
1	824.5
2	604.0
3	402.5
4	201.0
5	220.0
6	239.0
7	260.0
8	281.0
9	299.5
10	318.0
11	418.5
12	519.0
13	496.0
14	473.0
15	489.0
16	505.0
17	543.0
18	581.0
19	613.5
20	646.0
21	585.0
22	524.0
23	623.5
24	723.0
25	782.5
26	1043.0
27	1244.0
28	1554.0
29	1864.0
30	2336.0
31	2808.0
32	2979.0
33	3150.0
34	3372.0
35	3594.0
36	4146.5
37	4699.0
38	4903.0
39	5107.0
40	5810.0
41	6513.0
42	7094.5
43	7676.0
44	8987.0
45	10298.0
46	11482.5
47	12667.0
48	13725.0
49	14783.0
50	14423.5
51	14064.0
52	13854.5
53	13645.0
54	12689.5
55	11734.0
56	10325.0
57	8916.0
58	8730.0
59	8544.0
60	8318.5
61	8093.0
62	7273.5
63	6454.0
64	5961.0
65	5468.0
66	4796.0
67	4124.0
68	3680.0
69	3236.0
70	2730.5
71	2225.0
72	2000.5
73	1776.0
74	1521.5
75	1006.0
76	745.0
77	686.0
78	627.0
79	484.5
80	342.0
81	269.5
82	197.0
83	122.5
84	48.0
85	44.5
86	41.0
87	29.0
88	17.0
89	13.0
90	9.0
91	7.0
92	5.0
93	2.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	173482.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.60626462687772
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.90545557401973	25.40205900323953
2	8.57405508609412	5.591358181252234
3	2.7772867093306934	2.71670836167441
4	1.3453311176325	1.7546488972919378
5	0.7866916522292542	1.282553809617136
6	0.6470317858784429	1.2658373779412273
7	0.4720149913375526	1.0773452000783943
8	0.4454972951949934	1.1620802158148973
9	0.4048368277764028	1.1880195063464798
>10	6.208676590177845	48.141593940581735
>50	0.4013011349573949	7.937999331342733
>100	0.028285542552063076	1.51427813836594
>500	0.0035356928190078845	0.9655180364533497
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	880	0.5072572370620583	No Hit
CTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGCT	795	0.4582607993912913	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	240	0.13834288283510682	No Hit
AAACTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCT	238	0.1371900254781476	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	229	0.13200216737183107	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTT	210	0.12105002248071846	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.49399937745702727	0.0
2	0.0	0.0	0.0	0.758580140879169	0.0
3	0.0	0.0	0.0	1.0277723337291478	0.0
4	0.0	0.0	0.0	2.2186739834680256	0.0
5	0.0	0.0	0.0	2.422729735649808	0.0
6	0.0	0.0	0.0	3.5087213659053966	0.0
7	0.0	0.0	0.0	4.284017938460474	0.0
8	0.0	0.0	0.0	4.921548056858925	0.0
9	0.0	0.0	0.0	5.810977507752965	0.0
10	0.0	0.0	0.0	7.279717780519016	0.0
11	0.0	0.0	0.0	8.4902180053262	0.0
12	0.0	0.0	0.0	9.124289551653774	0.0
13	0.0	0.0	0.0	9.389446743754394	0.0
14	0.0	0.0	0.0	9.673626082244844	0.0
15	0.0	0.0	0.0	9.910538269099964	0.0
16	0.0	0.0	0.0	10.42067764955442	0.0
17	0.0	0.0	0.0	11.010940616317543	0.0
18	0.0	0.0	0.0	11.961471507130423	0.0
19	0.0	0.0	0.0	12.309634428932108	0.0
20	0.0	0.0	0.0	12.774235943786675	0.0
21	0.0	0.0	0.0	13.12355172294532	0.0
22	0.0	0.0	0.0	13.55183823105567	0.0
23	0.0	0.0	0.0	13.947844733171165	0.0
24	0.0	0.0	0.0	14.222801212805939	0.0
25	0.0	0.0	0.0	14.477006260015449	0.0
26	0.0	0.0	0.0	14.717377018941447	0.0
27	0.0	0.0	0.0	15.040177078890029	0.0
28	0.0	0.0	0.0	15.331849990200713	0.0
29	0.0	0.0	0.0	15.58778432344566	0.0
30	0.0	0.0	0.0	16.043739408123034	0.0
31	0.0	0.0	0.0	16.307167314188217	0.0
32	0.0	0.0	0.0	16.58442950853691	0.0
33	0.0	0.0	0.0	16.842669556495775	0.0
34	0.0	0.0	0.0	17.0991803184192	0.0
35	0.0	0.0	0.0	17.359726081091985	0.0
36	0.0	0.0	0.0	17.60413184076734	0.0
37	0.0	0.0	0.0	17.838738312908543	0.0
38	0.0	0.0	0.0	18.107354077080043	0.0
39	0.0	0.0	0.0	18.373087697859145	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACAA	20	7.016713E-4	45.000004	37
TCTGCGG	20	7.016713E-4	45.000004	2
GGTACCT	30	2.1548185E-6	45.000004	8
TACCCGG	20	7.016713E-4	45.000004	2
GATAATA	25	3.877074E-5	45.000004	40
CACGGGT	25	3.877074E-5	45.000004	4
CTATCGG	20	7.016713E-4	45.000004	2
CTATATA	20	7.016713E-4	45.000004	25
GGGTACC	30	2.1548185E-6	45.000004	7
GGCAACG	30	2.1548185E-6	45.000004	1
AAGACTA	20	7.016713E-4	45.000004	16
ATAGCGG	25	3.877074E-5	45.000004	2
CCAATAG	20	7.016713E-4	45.000004	1
CCTACGA	20	7.016713E-4	45.000004	32
TTGTAAG	20	7.016713E-4	45.000004	1
CGATTGA	20	7.016713E-4	45.000004	15
AAGCGAC	25	3.877074E-5	45.000004	14
TGCGGGT	25	3.877074E-5	45.000004	4
TGACGAC	20	7.016713E-4	45.000004	22
ACGGGTC	20	7.016713E-4	45.000004	5
>>END_MODULE