##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553173_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	193307
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22043174846229	33.0	31.0	34.0	30.0	34.0
2	32.422379944854555	34.0	31.0	34.0	31.0	34.0
3	32.55648786645077	34.0	31.0	34.0	31.0	34.0
4	36.15516768663318	37.0	35.0	37.0	35.0	37.0
5	35.95535081502481	37.0	35.0	37.0	35.0	37.0
6	35.974631027329586	37.0	35.0	37.0	35.0	37.0
7	35.9319217617572	37.0	35.0	37.0	35.0	37.0
8	35.972851474597405	37.0	35.0	37.0	35.0	37.0
9	37.707299787384834	39.0	38.0	39.0	35.0	39.0
10	37.42455782770412	39.0	37.0	39.0	35.0	39.0
11	37.408288370312505	39.0	37.0	39.0	35.0	39.0
12	37.312984009891004	39.0	37.0	39.0	35.0	39.0
13	37.28364725540203	39.0	37.0	39.0	34.0	39.0
14	38.50637069531884	40.0	38.0	41.0	34.0	41.0
15	38.54130476392474	40.0	38.0	41.0	35.0	41.0
16	38.46017992105821	40.0	38.0	41.0	34.0	41.0
17	38.40831940902295	40.0	38.0	41.0	34.0	41.0
18	38.31195973244632	40.0	37.0	41.0	34.0	41.0
19	38.327851552194176	40.0	37.0	41.0	34.0	41.0
20	38.23165224228817	40.0	37.0	41.0	34.0	41.0
21	38.20036522215957	40.0	37.0	41.0	34.0	41.0
22	38.17479449787126	40.0	37.0	41.0	34.0	41.0
23	38.05370731530674	40.0	37.0	41.0	34.0	41.0
24	37.91619548179838	40.0	37.0	41.0	33.0	41.0
25	37.90674419446787	40.0	36.0	41.0	33.0	41.0
26	37.87545717433926	40.0	36.0	41.0	33.0	41.0
27	37.83812795191069	40.0	36.0	41.0	33.0	41.0
28	37.75056257662682	40.0	36.0	41.0	33.0	41.0
29	37.53308467877521	40.0	36.0	41.0	33.0	41.0
30	37.570863962505236	40.0	36.0	41.0	33.0	41.0
31	37.43451090752016	40.0	36.0	41.0	33.0	41.0
32	37.358631606718845	40.0	36.0	41.0	33.0	41.0
33	37.30854030117895	40.0	36.0	41.0	33.0	41.0
34	37.156248868380345	39.0	35.0	41.0	32.0	41.0
35	37.057618192822815	39.0	35.0	41.0	32.0	41.0
36	36.980321457577844	39.0	35.0	41.0	32.0	41.0
37	36.85992229976152	39.0	35.0	41.0	31.0	41.0
38	36.8004366111936	39.0	35.0	41.0	31.0	41.0
39	36.69847444738163	39.0	35.0	41.0	31.0	41.0
40	36.585410771467146	39.0	35.0	41.0	31.0	41.0
41	36.45691051022467	39.0	35.0	41.0	31.0	41.0
42	36.367658698339945	39.0	35.0	40.0	31.0	41.0
43	36.291639723341625	39.0	35.0	40.0	31.0	41.0
44	36.20795935998179	38.0	35.0	40.0	31.0	41.0
45	36.0353479180785	38.0	35.0	40.0	30.0	41.0
46	35.87095656132473	38.0	35.0	40.0	30.0	41.0
47	35.75839985101419	38.0	35.0	40.0	30.0	41.0
48	35.63232578230483	38.0	35.0	40.0	29.0	41.0
49	35.42479061803245	38.0	34.0	40.0	28.0	41.0
50	35.377606605037585	37.0	34.0	40.0	28.0	41.0
51	34.04656841190438	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	1.0
13	1.0
14	3.0
15	5.0
16	12.0
17	31.0
18	63.0
19	133.0
20	276.0
21	404.0
22	613.0
23	807.0
24	1004.0
25	1067.0
26	1177.0
27	1264.0
28	1592.0
29	1912.0
30	2419.0
31	3220.0
32	4447.0
33	6484.0
34	11802.0
35	15676.0
36	17329.0
37	26729.0
38	42404.0
39	52417.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.990481462130187	33.245045445845214	20.074285980331805	26.690187111692797
2	23.124356593398065	31.336164753475042	20.124465228884624	25.415013424242268
3	20.820766966535096	32.34078434821295	19.76234694035912	27.07610174489284
4	20.34173620199993	31.50118723067452	18.978619501621772	29.17845706570378
5	19.324183811243255	33.65941223028654	16.92954730040816	30.086856658062043
6	19.55645682774033	36.28632175761871	19.00603702918156	25.151184385459395
7	82.65970709803577	7.3059951269224594	5.751990357307289	4.282307417734485
8	84.58979757587672	5.640250999705132	4.46543580936024	5.3045156150579125
9	77.23569244776444	9.20918538904437	8.541853114475938	5.013269048715256
10	38.172440728995845	30.713838609051926	13.350266674253907	17.763453987698323
11	27.45891250704837	29.38848567304857	23.571831335647442	19.580770484255613
12	27.13610991841992	24.230886620763865	29.12672588162871	19.506277579187508
13	26.755885715468143	24.14139167231399	26.084932258014454	23.01779035420342
14	20.266208673250322	28.651833611819537	28.345585002095113	22.736372712835024
15	20.054628130383275	29.024815449000812	28.372485217814152	22.54807120280176
16	22.733786153631268	27.400456269043545	27.3347576652682	22.530999912056988
17	21.98885710295023	28.355413927069378	26.104072796122228	23.551656173858163
18	23.364389287506402	27.303718954823157	25.427946220261035	23.903945537409406
19	23.01365185947741	30.64814000527658	24.784410290367138	21.553797844878872
20	25.943706125489506	27.541165089727738	24.683017169579994	21.83211161520276
21	24.599212651378377	28.803405981159504	24.289860170609444	22.307521196852676
22	22.09490603030413	26.872798191477802	25.331208906040654	25.70108687217742
23	23.479749827993814	27.628073478973857	24.911151691351062	23.981025001681264
24	22.227855173377066	28.419043283481717	25.012027500297457	24.341074042843765
25	22.165777752486974	28.52974801740237	24.469367379349947	24.835106850760706
26	24.204503716885576	28.332652206076347	24.06327758436063	23.39956649267745
27	20.83576900991687	26.321343769237533	26.84279410471426	26.000093116131335
28	22.505651631860204	27.67307960911917	26.78226861934643	23.039000139674197
29	22.66187980776692	30.086856658062043	24.123803069728464	23.12746046444257
30	22.850698629640934	26.455844847832722	26.892456041426332	23.80100048110001
31	24.38659748482983	28.495605435912825	21.544486231745356	25.573310847511987
32	24.961330939903885	26.653975282840246	24.982540725374662	23.402153051881207
33	22.358217757246244	25.733160206303964	25.64418256969484	26.26443946675495
34	22.38253141376153	26.03889150418764	26.401009792713147	25.177567289337688
35	22.84552551123343	27.35131164417222	26.01975096607986	23.78341187851449
36	23.4000838045182	28.12262359873155	25.426394284738784	23.050898312011466
37	23.639081874945038	29.054819535764352	23.38611638481793	23.919982204472678
38	25.15066707361865	27.07713636857434	25.900769242707195	21.871427315099815
39	23.99913091610754	25.098418577702823	24.9370172833886	25.96543322280104
40	25.935429136037495	26.294443553518498	23.778756071947733	23.991371238496278
41	21.647948599895503	25.242748581272277	26.837620986306753	26.271681832525463
42	22.908120243964262	26.785889802231683	25.716088915559187	24.589901038244864
43	21.936091295193656	26.056997418613914	26.439808180769447	25.567103105422976
44	24.511786950291505	23.524755958139128	25.090141588250813	26.87331550331856
45	25.519510416073913	24.01206371212631	26.232883444469163	24.23554242733062
46	22.105769578959894	24.545929531781052	28.289715323294036	25.058585565965018
47	22.48909765295618	26.218398712928142	25.177049977496935	26.115453656618747
48	23.217472724733195	24.341591354684518	27.99898606879213	24.441949851790156
49	22.170433559053734	25.157909439389158	27.13610991841992	25.535547083137182
50	21.93195280046765	23.417672407103726	27.47029336754489	27.180081424883735
51	22.02558624364353	23.767892523291966	28.605275546151976	25.601245686912527
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1164.0
1	926.5
2	689.0
3	488.0
4	287.0
5	275.5
6	264.0
7	294.5
8	325.0
9	346.0
10	367.0
11	445.5
12	524.0
13	547.5
14	571.0
15	539.5
16	508.0
17	595.0
18	682.0
19	673.5
20	665.0
21	624.0
22	583.0
23	708.5
24	834.0
25	882.5
26	1173.0
27	1415.0
28	1756.0
29	2097.0
30	2531.0
31	2965.0
32	3138.0
33	3311.0
34	3659.0
35	4007.0
36	4484.5
37	4962.0
38	5226.0
39	5490.0
40	6263.0
41	7036.0
42	7796.0
43	8556.0
44	10245.0
45	11934.0
46	13130.5
47	14327.0
48	15655.5
49	16984.0
50	16529.0
51	16074.0
52	15677.0
53	15280.0
54	14079.5
55	12879.0
56	11377.5
57	9876.0
58	9767.5
59	9659.0
60	9225.0
61	8791.0
62	7946.5
63	7102.0
64	6524.0
65	5946.0
66	5274.5
67	4603.0
68	4058.5
69	3514.0
70	3030.5
71	2547.0
72	2240.5
73	1934.0
74	1670.0
75	1092.5
76	779.0
77	729.5
78	680.0
79	532.5
80	385.0
81	310.0
82	235.0
83	150.5
84	66.0
85	57.0
86	48.0
87	33.5
88	19.0
89	11.5
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	193307.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.07023025550032
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.10594573668419	25.048756640990753
2	8.737942381520792	5.604556482693333
3	2.766396748072394	2.6615694206624694
4	1.334000064522373	1.7112675692033914
5	0.7952382488627933	1.275173687450532
6	0.6048972481207859	1.1639516416891265
7	0.4613349679001194	1.0356583051829473
8	0.38552117946898085	0.9891002395153823
9	0.31938574700777494	0.9218497002177882
>10	5.921540794270413	46.225434153962354
>50	0.5306965190179695	10.442456817394092
>100	0.03387424589476401	1.907846068688666
>500	0.0032261186566441918	1.0123792723491647
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGCT	1000	0.5173118407507229	Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	957	0.49506743159844185	No Hit
AAACTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCT	337	0.17433409033299363	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTT	275	0.1422607562064488	No Hit
CCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	268	0.13863957332119375	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	248	0.12829333650617927	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	203	0.10501430367239675	No Hit
AATCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCT	196	0.10139312078714169	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5783546379593083	0.0
2	0.0	0.0	0.0	0.8577030319646987	0.0
3	0.0	0.0	0.0	1.176367125867144	0.0
4	0.0	0.0	0.0	2.6486366246437014	0.0
5	0.0	0.0	0.0	2.892290501637292	0.0
6	0.0	0.0	0.0	4.145737091776294	0.0
7	0.0	0.0	0.0	5.03758270523054	0.0
8	0.0	0.0	0.0	5.759750034918549	0.0
9	0.0	0.0	0.0	6.736952102096665	0.0
10	0.0	0.0	0.0	8.306993538775108	0.0
11	0.0	0.0	0.0	9.619930990600444	0.0
12	0.0	0.0	0.0	10.286745953328126	0.0
13	0.0	0.0	0.0	10.598684993300811	0.0
14	0.0	0.0	0.0	10.905968226706742	0.0
15	0.0	0.0	0.0	11.176005007578619	0.0
16	0.0	0.0	0.0	11.72745942981889	0.0
17	0.0	0.0	0.0	12.308917938822702	0.0
18	0.0	0.0	0.0	13.28146419943406	0.0
19	0.0	0.0	0.0	13.641513240596565	0.0
20	0.0	0.0	0.0	14.144340349806267	0.0
21	0.0	0.0	0.0	14.530772294847056	0.0
22	0.0	0.0	0.0	14.962727681873911	0.0
23	0.0	0.0	0.0	15.364678982137223	0.0
24	0.0	0.0	0.0	15.687998882606424	0.0
25	0.0	0.0	0.0	15.959587599000553	0.0
26	0.0	0.0	0.0	16.227037820668677	0.0
27	0.0	0.0	0.0	16.5658770763604	0.0
28	0.0	0.0	0.0	16.877816116333086	0.0
29	0.0	0.0	0.0	17.161303005064482	0.0
30	0.0	0.0	0.0	17.651197318255416	0.0
31	0.0	0.0	0.0	17.93054571226081	0.0
32	0.0	0.0	0.0	18.233173139099982	0.0
33	0.0	0.0	0.0	18.517177339672127	0.0
34	0.0	0.0	0.0	18.76031390482497	0.0
35	0.0	0.0	0.0	19.061389396141887	0.0
36	0.0	0.0	0.0	19.329356929650764	0.0
37	0.0	0.0	0.0	19.56369919351084	0.0
38	0.0	0.0	0.0	19.876155545324277	0.0
39	0.0	0.0	0.0	20.16688479982618	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGTAC	85	0.0	45.000004	6
CCACAAT	20	7.018717E-4	45.0	20
GCCTTAT	20	7.018717E-4	45.0	25
GGTAATT	20	7.018717E-4	45.0	8
GGTAATC	20	7.018717E-4	45.0	8
CGAAATT	20	7.018717E-4	45.0	37
TACCACG	20	7.018717E-4	45.0	1
TGCCAGT	45	3.8198777E-10	45.0	24
GAATGCG	20	7.018717E-4	45.0	12
CATGCGG	20	7.018717E-4	45.0	2
CTATATG	35	1.204935E-7	45.0	1
TATGGGT	20	7.018717E-4	45.0	4
TAGCGTG	20	7.018717E-4	45.0	17
CGGTTAC	20	7.018717E-4	45.0	33
GGCAACG	40	6.7648216E-9	45.0	1
TCTTACG	20	7.018717E-4	45.0	1
ATAGCGG	20	7.018717E-4	45.0	2
CCGGAAT	20	7.018717E-4	45.0	43
AGGACCG	20	7.018717E-4	45.0	19
ACGGGTC	35	1.204935E-7	45.0	5
>>END_MODULE