FastQCFastQC Report
Sat 18 Jun 2016
SRR3553172_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553172_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1416452
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT101750.7183441443832901No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGT75100.5301979876480106No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCC72670.5130424469025424No Hit
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT51370.36266671938053674TruSeq Adapter, Index 20 (95% over 23bp)
AACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTG25300.17861530076557483TruSeq Adapter, Index 23 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTT22000.15531765283963028No Hit
AATGATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCT20060.14162145981649923No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTGC453.8562575E-1045.00000415
CGTATAG253.8912673E-545.01
TCGATAG406.8175723E-945.01
AATTGCG406.8175723E-945.01
TGTTCGA207.0338434E-445.015
CGAGCTA207.0338434E-445.027
ACTCACG502.1827873E-1145.01
TTCGATC207.0338434E-445.017
TATCGTG207.0338434E-445.044
TACACGA207.0338434E-445.039
CGAAACG1150.043.0434761
ACGATAG750.042.01
CACTACG650.041.538461
TCTAGCG603.6379788E-1241.2499961
CCGTACC501.0822987E-940.545
GTATACG501.0822987E-940.51
CATGACG900.040.0000041
TACGAGT403.4589902E-739.37537
ATACGCG403.4589902E-739.3751
TCACGCG1200.039.3749961