##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553170_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	494558
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.848808430962595	33.0	31.0	34.0	30.0	34.0
2	32.06900100695975	33.0	31.0	34.0	30.0	34.0
3	32.234914812822765	34.0	31.0	34.0	30.0	34.0
4	35.87819224438792	37.0	35.0	37.0	35.0	37.0
5	35.64715564200761	37.0	35.0	37.0	33.0	37.0
6	34.96910170293474	37.0	35.0	37.0	33.0	37.0
7	35.39371721820292	37.0	35.0	37.0	33.0	37.0
8	35.8015662470327	37.0	35.0	37.0	35.0	37.0
9	37.46238863793529	39.0	37.0	39.0	35.0	39.0
10	37.04416671047683	39.0	37.0	39.0	33.0	39.0
11	37.027808669559484	39.0	37.0	39.0	33.0	39.0
12	36.87521787131135	39.0	37.0	39.0	33.0	39.0
13	36.85648194953878	39.0	37.0	39.0	33.0	39.0
14	38.043258829095876	40.0	37.0	41.0	33.0	41.0
15	38.0461422118336	40.0	37.0	41.0	33.0	41.0
16	37.95400741672362	40.0	37.0	41.0	33.0	41.0
17	37.93336069783524	40.0	37.0	41.0	33.0	41.0
18	37.94509440753157	40.0	37.0	41.0	33.0	41.0
19	37.96550455153895	40.0	37.0	41.0	33.0	41.0
20	37.84199426558664	40.0	37.0	41.0	33.0	41.0
21	37.80461947840294	40.0	36.0	41.0	33.0	41.0
22	37.81926285693488	40.0	36.0	41.0	33.0	41.0
23	37.72814310960494	40.0	36.0	41.0	33.0	41.0
24	37.574634319938205	39.0	36.0	41.0	33.0	41.0
25	37.600742481165	39.0	36.0	41.0	33.0	41.0
26	37.53730603892769	39.0	36.0	41.0	33.0	41.0
27	37.44260733826973	39.0	36.0	41.0	32.0	41.0
28	37.349471649432424	39.0	36.0	41.0	32.0	41.0
29	37.138564132012824	39.0	35.0	41.0	32.0	41.0
30	37.16991333675727	39.0	35.0	41.0	32.0	41.0
31	37.13212808204498	39.0	35.0	41.0	32.0	41.0
32	37.04774970781991	39.0	35.0	41.0	31.0	41.0
33	36.818084026544916	39.0	35.0	41.0	31.0	41.0
34	36.766931280052084	39.0	35.0	41.0	31.0	41.0
35	36.727750840144125	39.0	35.0	41.0	31.0	41.0
36	36.63589508207328	39.0	35.0	40.0	31.0	41.0
37	36.59221365340365	39.0	35.0	40.0	31.0	41.0
38	36.41766789739525	39.0	35.0	40.0	30.0	41.0
39	36.423903768617635	39.0	35.0	40.0	30.0	41.0
40	36.337541805005685	38.0	35.0	40.0	30.0	41.0
41	36.3047589969225	38.0	35.0	40.0	30.0	41.0
42	36.24037018913859	38.0	35.0	40.0	30.0	41.0
43	36.1445492743015	38.0	35.0	40.0	30.0	41.0
44	36.030849768884536	38.0	35.0	40.0	30.0	41.0
45	35.83458360798936	38.0	35.0	40.0	30.0	41.0
46	35.63947201339378	38.0	34.0	40.0	29.0	41.0
47	35.508183064473734	38.0	34.0	40.0	29.0	41.0
48	35.41435584906118	37.0	34.0	40.0	29.0	41.0
49	35.344307442200915	37.0	34.0	40.0	28.0	41.0
50	35.265997112573245	37.0	34.0	40.0	28.0	41.0
51	34.14004221951723	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	5.0
11	10.0
12	4.0
13	11.0
14	7.0
15	19.0
16	52.0
17	102.0
18	196.0
19	381.0
20	753.0
21	967.0
22	1418.0
23	1856.0
24	2433.0
25	2901.0
26	3325.0
27	4097.0
28	4979.0
29	6672.0
30	8780.0
31	11400.0
32	14915.0
33	20733.0
34	32865.0
35	40690.0
36	48668.0
37	69793.0
38	101981.0
39	114512.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.837915876398725	31.307753590074373	21.92179683677142	26.932533696755485
2	24.041265129671345	30.162084123601275	19.78938769567978	26.0072630510476
3	20.335936331026897	31.140735768100004	19.894329886484496	28.628998014388607
4	21.06992506440094	31.33060227516287	19.95680991916014	27.642662741276048
5	18.21667023887997	33.203385649408155	17.413731048734427	31.166213062977448
6	19.75663117369449	38.11362873515341	19.210891341359353	22.918848749792744
7	86.07584954646372	5.168049045814647	4.665580174620571	4.0905212331010725
8	87.06238702032927	4.360459238350203	4.02116637482358	4.555987366496953
9	80.38794236469737	7.5558377379397355	7.6021417103757285	4.454078186987168
10	37.346681279041086	32.40085086076861	13.029816523036732	17.22265133715358
11	29.1444077337744	29.25116973135608	22.57773607948916	19.02668645538036
12	27.254235094771495	24.129829059483416	27.746998329821782	20.868937515923307
13	26.923232462117692	24.962896161825306	25.227172545990562	22.886698830066443
14	22.364616485831792	27.20146069823964	27.726980455275214	22.70694236065335
15	21.905822977284767	27.871958395173063	27.774497632229185	22.447720995312988
16	23.680134584821193	27.562186841583795	25.69102107336248	23.06665750023253
17	22.725140428422954	27.443899401081367	26.202184576935366	23.62877559356031
18	25.995939808879847	25.952871048491783	24.595092992126304	23.456096150502066
19	24.790014518014065	29.77325207559073	23.63484161615018	21.801891790245026
20	26.63064797253305	27.743965318526847	23.78285256734296	21.842534141597145
21	25.44979557503872	28.374022864861146	24.073819450903635	22.102362109196495
22	24.18866947860514	26.10310620796752	24.743103943319085	24.965120370108256
23	24.433130188976822	27.76357879156742	23.525046607273563	24.27824441218219
24	21.667630490255945	27.431160753642647	26.417730579628678	24.48347817647273
25	23.926415101969837	27.81594878659328	23.816215691587235	24.441420419849642
26	25.72317099308878	26.315619199365898	24.024280266419712	23.93692954112561
27	23.445581711346293	26.49133165371908	23.747063034062737	26.31602360087189
28	22.91035631816693	28.390401125853792	26.615280715305385	22.083961840673894
29	26.225235462776865	27.68997771747702	22.824420998143797	23.26036582160232
30	25.115153328830996	26.125348290797035	25.57900185620291	23.180496524169055
31	28.138256786868276	27.086812871291134	21.778032101391545	22.99689824044905
32	26.239389515486554	25.44959337428573	24.351036683260606	23.95998042696711
33	24.388241621811797	25.678889028182738	23.903566416881336	26.02930293312412
34	24.378940387174	26.608405889703533	26.608405889703533	22.404247833418932
35	25.039732447963637	25.369521876099466	26.363742978578852	23.227002697358046
36	25.53957270936877	30.42130548894164	23.66517172909952	20.37395007259007
37	25.920721128765482	28.013701123022983	22.9485722604831	23.117005487728438
38	25.73732504579847	27.03686928530122	24.403608879039464	22.822196789860847
39	24.845013122828867	25.561612591445293	24.289163252843952	25.304211032881884
40	25.166310119338885	25.809915116123893	25.40187399657876	23.621900767958458
41	22.743945098451547	24.61955928323877	27.596965371099042	25.03953024721064
42	25.564039000481237	24.652518005977054	25.718520375769877	24.064922617771828
43	23.663554123075553	24.306956919107567	26.911100416937956	25.11838854087893
44	25.91263309864566	23.645153854552955	24.25256491655175	26.189648130249637
45	26.371831008698678	23.00417746755689	25.89524383388804	24.728747689856398
46	22.857784122388072	24.889092886981913	27.001888555032977	25.251234435597038
47	23.153806024773633	26.182368903141796	26.475357794232423	24.188467277852144
48	23.74241241674384	25.055099705191303	28.173237517136513	23.029250360928344
49	23.927426105734817	24.449912851475457	27.2952818476296	24.327379195160123
50	22.428916325284394	24.315853752239374	26.640960210935827	26.614269711540405
51	23.427181442823695	22.055047132995522	28.782063984406275	25.735707439774508
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1429.0
1	1188.0
2	947.0
3	665.0
4	383.0
5	385.0
6	387.0
7	450.0
8	513.0
9	563.5
10	614.0
11	756.5
12	899.0
13	983.0
14	1067.0
15	1082.5
16	1098.0
17	1080.5
18	1063.0
19	1163.5
20	1264.0
21	1408.5
22	1553.0
23	1725.0
24	1897.0
25	2426.5
26	3117.0
27	3278.0
28	4146.5
29	5015.0
30	5224.0
31	5433.0
32	6749.5
33	8066.0
34	8539.0
35	9012.0
36	9939.5
37	10867.0
38	12203.5
39	13540.0
40	15291.5
41	17043.0
42	19648.0
43	22253.0
44	26240.0
45	30227.0
46	34665.0
47	39103.0
48	40364.0
49	41625.0
50	42768.5
51	43912.0
52	41207.5
53	38503.0
54	36262.0
55	34021.0
56	31883.5
57	29746.0
58	27024.5
59	24303.0
60	24561.0
61	24819.0
62	21680.5
63	18542.0
64	17349.0
65	16156.0
66	14346.0
67	12536.0
68	11105.5
69	9675.0
70	7900.5
71	6126.0
72	5480.0
73	4834.0
74	4005.0
75	3046.0
76	2916.0
77	2364.0
78	1812.0
79	1465.5
80	1119.0
81	743.0
82	367.0
83	339.5
84	312.0
85	174.5
86	37.0
87	36.0
88	35.0
89	25.5
90	16.0
91	18.5
92	21.0
93	29.5
94	38.0
95	20.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	494558.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.019149016735906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.9804235086666	19.769459616267003
2	9.959448434714963	5.182727458946915
3	3.7613833173942535	2.9360397913303653
4	1.9079653736559268	1.9857454150370304
5	1.0612876942062393	1.3806901332590094
6	0.6881755337891983	1.0743445057999754
7	0.45969192546084103	0.8372554897249115
8	0.32866065381956666	0.6841176422135326
9	0.2776689062479234	0.650223778408091
>10	3.7139873362945193	24.14093907651071
>50	1.4730447277616556	27.224882151090213
>100	0.382772005935936	12.857016362599277
>500	0.003921844323114099	0.5826658261408448
>1k	0.0015687377292456395	0.6938927526721094
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGCT	2222	0.4492900731562324	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1178	0.2381924870288217	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	606	0.12253365631533612	No Hit
AAACTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCT	604	0.1221292548093449	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGT	559	0.11303022092454273	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCC	558	0.11282802017154711	No Hit
AATCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCT	528	0.106761997581679	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.49862705688715986	0.0
2	0.0	0.0	0.0	0.7085114384965969	0.0
3	0.0	0.0	0.0	0.9651041940480186	0.0
4	0.0	0.0	0.0	2.081454551336749	0.0
5	0.0	0.0	0.0	2.262424225267815	0.0
6	0.0	0.0	0.0	3.3593633102689675	0.0
7	0.0	0.0	0.0	4.1356120010190915	0.0
8	0.0	0.0	0.0	4.969487906372963	0.0
9	0.0	0.0	0.0	5.938433914727899	0.0
10	0.0	0.0	0.0	7.434719486895369	0.0
11	0.0	0.0	0.0	8.590094589512251	0.0
12	0.0	0.0	0.0	9.237945802110167	0.0
13	0.0	0.0	0.0	9.504244193805377	0.0
14	0.0	0.0	0.0	9.78105702465636	0.0
15	0.0	0.0	0.0	10.007319667258441	0.0
16	0.0	0.0	0.0	10.483098039057097	0.0
17	0.0	0.0	0.0	11.032275284193158	0.0
18	0.0	0.0	0.0	11.939550062884434	0.0
19	0.0	0.0	0.0	12.238200575058942	0.0
20	0.0	0.0	0.0	12.64300648255614	0.0
21	0.0	0.0	0.0	13.020515288398935	0.0
22	0.0	0.0	0.0	13.408336332644502	0.0
23	0.0	0.0	0.0	13.794944172372098	0.0
24	0.0	0.0	0.0	14.085708855179776	0.0
25	0.0	0.0	0.0	14.354433655910935	0.0
26	0.0	0.0	0.0	14.589390930891827	0.0
27	0.0	0.0	0.0	14.91675394999171	0.0
28	0.0	0.0	0.0	15.203676818492472	0.0
29	0.0	0.0	0.0	15.501720728407992	0.0
30	0.0	0.0	0.0	15.978914505477618	0.0
31	0.0	0.0	0.0	16.233080851993094	0.0
32	0.0	0.0	0.0	16.53031595889663	0.0
33	0.0	0.0	0.0	16.809150797277567	0.0
34	0.0	0.0	0.0	17.055026912920223	0.0
35	0.0	0.0	0.0	17.32658252419332	0.0
36	0.0	0.0	0.0	17.59025230609959	0.0
37	0.0	0.0	0.0	17.84583405788603	0.0
38	0.0	0.0	0.0	18.138822948976664	0.0
39	0.0	0.0	0.0	18.464770562805576	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAAA	25	3.8875707E-5	45.000004	43
ACGCCTA	25	3.8875707E-5	45.000004	12
CTCAATC	25	3.8875707E-5	45.000004	22
ATAGGTT	35	1.2100463E-7	45.000004	45
ATTCGCG	35	1.2100463E-7	45.000004	1
CGCTATA	25	3.8875707E-5	45.000004	19
TGTTCGG	25	3.8875707E-5	45.000004	2
TCGCTAT	25	3.8875707E-5	45.000004	23
TCGAGTA	25	3.8875707E-5	45.000004	38
AATAGCG	35	1.2100463E-7	45.000004	1
ATTGCGG	50	2.1827873E-11	45.000004	2
CCTATCA	25	3.8875707E-5	45.000004	26
TATCGGA	25	3.8875707E-5	45.000004	21
TATCCCG	25	3.8875707E-5	45.000004	1
CTAGACA	20	7.029384E-4	45.0	31
TCGCACT	20	7.029384E-4	45.0	29
GGTCGCA	20	7.029384E-4	45.0	9
CGACGTT	20	7.029384E-4	45.0	24
ACGTTTG	20	7.029384E-4	45.0	24
ACCGCGA	20	7.029384E-4	45.0	26
>>END_MODULE