FastQCFastQC Report
Sat 18 Jun 2016
SRR3553169_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553169_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504239
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGCT24660.48905380186776515No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12870.2552361082740526No Hit
AAACTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCT6790.13465836636991585No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCC6580.1304936746265164No Hit
AATCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCT6330.12553570826532656No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGT6160.12216429113971747No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG5880.11661136881518486No Hit
AACCCTGGGAATGATACCTGTCTCTTATACACATCTGACGCTGAGGCATTC5750.11403322630736616No Hit
AACCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCT5240.10391897493053888No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCAG207.029516E-445.013
ACTATTG207.029516E-445.01
TCGTTTA207.029516E-445.031
CGTATCT207.029516E-445.021
GTACAAT207.029516E-445.030
TCCGATT207.029516E-445.015
CCGATCG351.2101191E-745.026
CGTGCCG207.029516E-445.034
ACGCAGT207.029516E-445.036
ACGCACG207.029516E-445.01
TCCGAAC207.029516E-445.041
GTCCTCG207.029516E-445.022
CGCATTG207.029516E-445.01
TAGAACG351.2101191E-745.01
ATTAACG453.8380676E-1045.01
CTGACGT207.029516E-445.026
CCGTATG253.887681E-545.035
GTAGCGT207.029516E-445.029
TACGCCT207.029516E-445.042
ATATAGA453.8380676E-1045.041