##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553169_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	504239
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.852817810601717	33.0	31.0	34.0	30.0	34.0
2	32.068967295270696	33.0	31.0	34.0	30.0	34.0
3	32.21106459436894	34.0	31.0	34.0	30.0	34.0
4	35.85853930378253	37.0	35.0	37.0	35.0	37.0
5	35.64687975345025	37.0	35.0	37.0	33.0	37.0
6	35.66827436989205	37.0	35.0	37.0	33.0	37.0
7	35.760456846852385	37.0	35.0	37.0	35.0	37.0
8	35.79957123506908	37.0	35.0	37.0	35.0	37.0
9	37.490291310271516	39.0	37.0	39.0	35.0	39.0
10	37.053690809318596	39.0	37.0	39.0	33.0	39.0
11	37.03359518006342	39.0	37.0	39.0	33.0	39.0
12	36.914905431749624	39.0	37.0	39.0	33.0	39.0
13	36.886056017087135	39.0	37.0	39.0	33.0	39.0
14	38.05979505750249	40.0	37.0	41.0	33.0	41.0
15	38.06170486614482	40.0	37.0	41.0	33.0	41.0
16	37.98379339955854	40.0	37.0	41.0	33.0	41.0
17	37.93123697294339	40.0	37.0	41.0	33.0	41.0
18	37.90418630847673	40.0	37.0	41.0	33.0	41.0
19	37.946527341201296	40.0	37.0	41.0	33.0	41.0
20	37.83233347678382	40.0	37.0	41.0	33.0	41.0
21	37.779556916462234	40.0	36.0	41.0	33.0	41.0
22	37.78431259779589	40.0	36.0	41.0	33.0	41.0
23	37.653933947988946	39.0	36.0	41.0	33.0	41.0
24	37.52331334942359	39.0	36.0	41.0	32.0	41.0
25	37.5500546367893	39.0	36.0	41.0	32.0	41.0
26	37.483847937188514	39.0	36.0	41.0	33.0	41.0
27	37.40608322640652	39.0	36.0	41.0	32.0	41.0
28	37.30895468220427	39.0	36.0	41.0	32.0	41.0
29	37.153679108517984	39.0	35.0	41.0	32.0	41.0
30	37.1636366881578	39.0	35.0	41.0	32.0	41.0
31	37.09823912866716	39.0	35.0	41.0	32.0	41.0
32	36.99532562931467	39.0	35.0	41.0	31.0	41.0
33	36.906163545461574	39.0	35.0	41.0	31.0	41.0
34	36.81053032391386	39.0	35.0	41.0	31.0	41.0
35	36.76017920073616	39.0	35.0	41.0	31.0	41.0
36	36.629699408415455	39.0	35.0	40.0	31.0	41.0
37	36.589163075446365	39.0	35.0	40.0	31.0	41.0
38	36.51998357921541	39.0	35.0	40.0	31.0	41.0
39	36.47343025827038	39.0	35.0	40.0	30.0	41.0
40	36.36809727133363	39.0	35.0	40.0	30.0	41.0
41	36.29000930114489	38.0	35.0	40.0	30.0	41.0
42	36.20469261600154	38.0	35.0	40.0	30.0	41.0
43	36.09497083724187	38.0	35.0	40.0	30.0	41.0
44	36.00067428342512	38.0	35.0	40.0	30.0	41.0
45	35.827879239804936	38.0	35.0	40.0	30.0	41.0
46	35.641884106544715	38.0	34.0	40.0	29.0	41.0
47	35.47456860734691	37.0	34.0	40.0	29.0	41.0
48	35.4189640230129	37.0	34.0	40.0	29.0	41.0
49	35.278754717504995	37.0	34.0	40.0	28.0	41.0
50	35.20926187780001	37.0	34.0	40.0	28.0	41.0
51	34.021172499548825	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	1.0
10	2.0
11	11.0
12	7.0
13	10.0
14	17.0
15	30.0
16	55.0
17	90.0
18	194.0
19	385.0
20	677.0
21	1074.0
22	1458.0
23	1836.0
24	2414.0
25	2887.0
26	3422.0
27	4136.0
28	5259.0
29	6653.0
30	8791.0
31	11437.0
32	15257.0
33	21173.0
34	33632.0
35	41136.0
36	49172.0
37	71076.0
38	105041.0
39	116875.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.698991946279442	31.369251485902517	21.942967521353964	26.988789046464078
2	23.931706988154428	30.33303651641384	19.78803702212641	25.947219473305317
3	20.310408357941373	30.975985594132943	19.95640955975242	28.757196488173264
4	20.96565319223622	31.50946277459697	19.95303814262681	27.571845890540004
5	18.189985304587704	33.29690880713312	17.423880342456652	31.08922554582252
6	20.21858682093214	36.743488702777846	19.660319808662162	23.377604667627853
7	85.80335912136904	5.186231132459013	4.826084455982183	4.184325290189771
8	86.58612285047369	4.463954592960877	4.139306955630167	4.810615600935271
9	80.06917354667132	7.524408068396138	7.889116073925262	4.51730231100728
10	37.09927236885683	32.38920432572649	13.07078587733198	17.4407374280847
11	28.84762979460137	29.22681506190517	22.782847022939517	19.142708120553944
12	27.04907791741615	23.993384089687627	27.922076634294452	21.035461358601772
13	27.093897933321305	24.86102820289585	25.241205063471885	22.803868800310966
14	22.311840218626486	27.129793609776314	27.97740753888533	22.58095863271187
15	21.635375288305745	27.920688403713317	28.03412667405734	22.409809633923597
16	23.672504506791423	27.42251194374096	25.760799938124578	23.144183611343035
17	22.58095863271187	27.541899773718413	26.267107462929285	23.610034130640432
18	25.940873276362996	25.818708985223278	24.562161990643325	23.6782557477704
19	24.570491374130125	29.90704804666041	23.739536211994707	21.782924367214754
20	26.594729880076713	27.678739645287255	23.886688653594824	21.83984182104121
21	25.07263420719143	28.44067991567491	24.212129565543325	22.274556311590338
22	24.004093297027797	25.939088408472966	24.912392734397777	25.144425560101457
23	24.502666394309045	27.707694168836603	23.529913394243604	24.259726042610747
24	21.685549907880986	27.443335402457965	26.388875116760108	24.482239572900948
25	23.938449822405644	27.58890129482249	23.93150866949998	24.54114021327188
26	25.868486967489623	26.29824349167756	24.061209069508706	23.772060471324114
27	23.480333730631706	26.414061585874954	23.793280567350006	26.312324116143337
28	23.019441177695498	28.254260380494173	26.567758543071836	22.158539898738493
29	26.323628279446847	27.5184981724936	22.882204668817764	23.27566887924179
30	25.183494335027635	26.076721554659592	25.59520386166084	23.14458024865193
31	28.14696998843802	27.05165605992396	21.731758154367277	23.06961579727074
32	26.400972554681413	25.338579522805656	24.307124201023722	23.953323721489213
33	24.450310269534885	25.663623797445258	23.791892336768875	26.094173596250986
34	24.22323541019239	26.733354619535575	26.582235804846512	22.461174165425525
35	25.10535678517528	25.138476000468028	26.40196414795365	23.354203066403034
36	25.700312748518062	30.325698726199285	23.686188493948308	20.287800031334346
37	26.020597375450926	27.896493527870714	22.870702186859802	23.21220690981856
38	25.721731163198402	27.005447813437677	24.504451262199076	22.768369761164845
39	24.901881845712055	25.496441171745936	24.35769545790786	25.24398152463415
40	25.260838610262198	25.819105622532174	25.173776720959708	23.746279046245927
41	22.700148144034873	24.632961750281115	27.662675834277	25.00421427140701
42	25.630702900806956	24.574656065873523	25.759808344852342	24.034832688467176
43	23.402196180779352	24.290068796741227	27.18393460244051	25.12380042003891
44	25.95435894486543	23.42202804622411	24.376139092771485	26.247473916138976
45	26.639351577327417	22.850870321415044	25.885145734463222	24.62463236679432
46	22.862769440681898	24.771388171085537	26.972725235453822	25.393117152778743
47	23.01765630980547	26.138993612156142	26.346434924708323	24.496915153330068
48	23.66556335388576	24.951858146632848	28.339537401906632	23.04304109757476
49	24.114755106209557	24.16473140713035	27.374915466673542	24.345598019986554
50	22.300139418014076	24.22045894903012	26.73811426724232	26.74128736571348
51	23.50195046396649	21.799781452842797	28.76671578358675	25.931552299603954
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1555.0
1	1272.5
2	990.0
3	705.5
4	421.0
5	419.5
6	418.0
7	439.0
8	460.0
9	513.5
10	567.0
11	732.0
12	897.0
13	988.5
14	1080.0
15	1093.0
16	1106.0
17	1122.0
18	1138.0
19	1200.0
20	1262.0
21	1344.5
22	1427.0
23	1622.0
24	1817.0
25	2395.0
26	3148.0
27	3323.0
28	4144.5
29	4966.0
30	5221.5
31	5477.0
32	6790.0
33	8103.0
34	8529.0
35	8955.0
36	9988.5
37	11022.0
38	12256.0
39	13490.0
40	15435.5
41	17381.0
42	19853.0
43	22325.0
44	26608.5
45	30892.0
46	35774.5
47	40657.0
48	42185.5
49	43714.0
50	44404.5
51	45095.0
52	42377.0
53	39659.0
54	36990.0
55	34321.0
56	32272.5
57	30224.0
58	27325.0
59	24426.0
60	24836.5
61	25247.0
62	21998.0
63	18749.0
64	17578.5
65	16408.0
66	14520.0
67	12632.0
68	11169.5
69	9707.0
70	7996.5
71	6286.0
72	5578.0
73	4870.0
74	4134.0
75	3167.5
76	2937.0
77	2427.5
78	1918.0
79	1515.0
80	1112.0
81	746.0
82	380.0
83	327.5
84	275.0
85	164.0
86	53.0
87	40.5
88	28.0
89	27.0
90	26.0
91	24.0
92	22.0
93	34.5
94	47.0
95	24.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	504239.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.713971770053355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.08430037233069	19.564295519183716
2	9.983960327626038	5.134545480358172
3	3.6201268985253865	2.792635226180778
4	1.8075416702953744	1.8591630193268138
5	1.0684507757560322	1.373705654274111
6	0.7072250126257917	1.0911338405841142
7	0.489911108271425	0.8818292285548883
8	0.34336641233388765	0.706345138683047
9	0.2836756647229526	0.6564985231083411
>10	3.689290808160534	23.45210615489947
>50	1.5036180813478488	27.345009326739255
>100	0.41078225933729895	13.442712994246337
>500	0.006200486933393191	0.9520510003486249
>1k	0.0015501217333482977	0.7479688935123278
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGCT	2466	0.48905380186776515	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1287	0.2552361082740526	No Hit
AAACTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCT	679	0.13465836636991585	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCC	658	0.1304936746265164	No Hit
AATCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCT	633	0.12553570826532656	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGT	616	0.12216429113971747	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	588	0.11661136881518486	No Hit
AACCCTGGGAATGATACCTGTCTCTTATACACATCTGACGCTGAGGCATTC	575	0.11403322630736616	No Hit
AACCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCT	524	0.10391897493053888	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.966373088951866E-4	0.0	0.0	0.5420048826052725	0.0
2	3.966373088951866E-4	0.0	0.0	0.7548007988275401	0.0
3	3.966373088951866E-4	0.0	0.0	1.0175730159706013	0.0
4	3.966373088951866E-4	0.0	0.0	2.250321772016841	0.0
5	3.966373088951866E-4	0.0	0.0	2.4452690093388254	0.0
6	3.966373088951866E-4	0.0	0.0	3.6427170448933937	0.0
7	3.966373088951866E-4	0.0	0.0	4.475457074918838	0.0
8	3.966373088951866E-4	0.0	0.0	5.368089338587455	0.0
9	3.966373088951866E-4	0.0	0.0	6.349568359448595	0.0
10	3.966373088951866E-4	0.0	0.0	7.887132887380786	0.0
11	3.966373088951866E-4	0.0	0.0	9.133168993275014	0.0
12	3.966373088951866E-4	0.0	0.0	9.814591889956946	0.0
13	3.966373088951866E-4	0.0	0.0	10.098980840434793	0.0
14	3.966373088951866E-4	0.0	0.0	10.392889086326127	0.0
15	3.966373088951866E-4	0.0	0.0	10.619765627014173	0.0
16	3.966373088951866E-4	0.0	0.0	11.109811022154178	0.0
17	3.966373088951866E-4	0.0	0.0	11.689298130450045	0.0
18	3.966373088951866E-4	0.0	0.0	12.661456174552146	0.0
19	3.966373088951866E-4	0.0	0.0	12.998994524421951	0.0
20	3.966373088951866E-4	0.0	0.0	13.407530952583993	0.0
21	5.949559633427799E-4	0.0	0.0	13.780964978908811	0.0
22	5.949559633427799E-4	0.0	0.0	14.185535033981902	0.0
23	5.949559633427799E-4	0.0	0.0	14.582172342877088	0.0
24	5.949559633427799E-4	0.0	0.0	14.891152806506438	0.0
25	5.949559633427799E-4	0.0	0.0	15.164435912335222	0.0
26	5.949559633427799E-4	0.0	0.0	15.40281493498123	0.0
27	5.949559633427799E-4	0.0	0.0	15.734998681180947	0.0
28	5.949559633427799E-4	0.0	0.0	16.049333748480382	0.0
29	5.949559633427799E-4	0.0	0.0	16.333722698958233	0.0
30	7.932746177903732E-4	0.0	0.0	16.836063850673987	0.0
31	7.932746177903732E-4	0.0	0.0	17.104587308796027	0.0
32	7.932746177903732E-4	0.0	0.0	17.399883785268493	0.0
33	7.932746177903732E-4	0.0	0.0	17.69260211923314	0.0
34	7.932746177903732E-4	0.0	0.0	17.969454960841983	0.0
35	7.932746177903732E-4	0.0	0.0	18.265148074623344	0.0
36	7.932746177903732E-4	0.0	0.0	18.545372333357793	0.0
37	7.932746177903732E-4	0.0	0.0	18.819448713804366	0.0
38	9.915932722379666E-4	0.0	0.0	19.123471211072527	0.0
39	9.915932722379666E-4	0.0	0.0	19.446730617822105	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCAG	20	7.029516E-4	45.0	13
ACTATTG	20	7.029516E-4	45.0	1
TCGTTTA	20	7.029516E-4	45.0	31
CGTATCT	20	7.029516E-4	45.0	21
GTACAAT	20	7.029516E-4	45.0	30
TCCGATT	20	7.029516E-4	45.0	15
CCGATCG	35	1.2101191E-7	45.0	26
CGTGCCG	20	7.029516E-4	45.0	34
ACGCAGT	20	7.029516E-4	45.0	36
ACGCACG	20	7.029516E-4	45.0	1
TCCGAAC	20	7.029516E-4	45.0	41
GTCCTCG	20	7.029516E-4	45.0	22
CGCATTG	20	7.029516E-4	45.0	1
TAGAACG	35	1.2101191E-7	45.0	1
ATTAACG	45	3.8380676E-10	45.0	1
CTGACGT	20	7.029516E-4	45.0	26
CCGTATG	25	3.887681E-5	45.0	35
GTAGCGT	20	7.029516E-4	45.0	29
TACGCCT	20	7.029516E-4	45.0	42
ATATAGA	45	3.8380676E-10	45.0	41
>>END_MODULE