##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553168_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	339475
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.700693718241403	31.0	31.0	34.0	30.0	34.0
2	31.909533839016127	33.0	31.0	34.0	30.0	34.0
3	32.091853597466674	34.0	31.0	34.0	30.0	34.0
4	35.77024228588261	37.0	35.0	37.0	35.0	37.0
5	35.511863907504235	37.0	35.0	37.0	33.0	37.0
6	34.85565947418809	37.0	35.0	37.0	32.0	37.0
7	35.267957876132265	37.0	35.0	37.0	33.0	37.0
8	35.665312615067386	37.0	35.0	37.0	33.0	37.0
9	37.308326091759334	39.0	37.0	39.0	34.0	39.0
10	36.900107518963104	39.0	37.0	39.0	32.0	39.0
11	36.914868547021136	39.0	37.0	39.0	33.0	39.0
12	36.786882686501215	39.0	37.0	39.0	33.0	39.0
13	36.75088592679873	39.0	37.0	39.0	33.0	39.0
14	37.912447161057514	40.0	37.0	41.0	33.0	41.0
15	37.926153619559614	40.0	37.0	41.0	33.0	41.0
16	37.83996759702482	40.0	37.0	41.0	33.0	41.0
17	37.829330583989986	40.0	37.0	41.0	33.0	41.0
18	37.813476691950804	40.0	37.0	41.0	33.0	41.0
19	37.82948081596583	40.0	36.0	41.0	33.0	41.0
20	37.715908387951984	39.0	36.0	41.0	33.0	41.0
21	37.70624346417262	39.0	36.0	41.0	33.0	41.0
22	37.668670741586276	39.0	36.0	41.0	33.0	41.0
23	37.55569924147581	39.0	36.0	41.0	32.0	41.0
24	37.366135945209514	39.0	36.0	41.0	32.0	41.0
25	37.42779880698137	39.0	36.0	41.0	32.0	41.0
26	37.39244716105752	39.0	36.0	41.0	32.0	41.0
27	37.295123352235066	39.0	36.0	41.0	32.0	41.0
28	37.2673422196038	39.0	36.0	41.0	32.0	41.0
29	37.059945504087196	39.0	35.0	41.0	31.0	41.0
30	37.05055747845939	39.0	35.0	41.0	31.0	41.0
31	37.049535311878635	39.0	35.0	41.0	31.0	41.0
32	36.960512556152885	39.0	35.0	41.0	31.0	41.0
33	36.73210987554312	39.0	35.0	41.0	31.0	41.0
34	36.686624935562264	39.0	35.0	41.0	30.0	41.0
35	36.64411517784815	39.0	35.0	41.0	30.0	41.0
36	36.61808969732675	39.0	35.0	40.0	30.0	41.0
37	36.52593563590839	39.0	35.0	40.0	30.0	41.0
38	36.38425804551145	39.0	35.0	40.0	30.0	41.0
39	36.37096693423669	39.0	35.0	40.0	30.0	41.0
40	36.27516606524781	39.0	35.0	40.0	30.0	41.0
41	36.21302599602327	39.0	35.0	40.0	30.0	41.0
42	36.13682303556963	38.0	35.0	40.0	30.0	41.0
43	36.04610648795935	38.0	35.0	40.0	30.0	41.0
44	35.96230355696296	38.0	35.0	40.0	29.0	41.0
45	35.751003755799395	38.0	35.0	40.0	28.0	41.0
46	35.60615361955961	38.0	34.0	40.0	28.0	41.0
47	35.484321378599304	38.0	34.0	40.0	28.0	41.0
48	35.39506296487223	38.0	34.0	40.0	28.0	41.0
49	35.27448265704397	38.0	34.0	40.0	27.0	41.0
50	35.19545769202445	37.0	34.0	40.0	27.0	41.0
51	33.91228809190662	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	6.0
13	8.0
14	11.0
15	25.0
16	48.0
17	90.0
18	165.0
19	368.0
20	545.0
21	868.0
22	1204.0
23	1514.0
24	1923.0
25	2220.0
26	2630.0
27	3192.0
28	3764.0
29	4783.0
30	6108.0
31	8172.0
32	10634.0
33	14378.0
34	22138.0
35	27592.0
36	32618.0
37	47176.0
38	68776.0
39	78497.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.737020399145738	32.967081522939836	20.95942263789675	25.336475440017676
2	22.49090507401134	32.2978127991752	20.44421533249871	24.76706679431475
3	20.998306208115473	32.2745415715443	21.11613520877826	25.61101701156197
4	20.909639885116725	31.75049709109655	19.818543338979307	27.521319684807423
5	19.10273215995287	34.36571176080713	18.38898298843803	28.142573090801974
6	20.31666543928124	39.11716621253406	19.1463288901981	21.419839457986598
7	81.76213270491199	7.254142425804551	6.077030709183298	4.906694160100155
8	83.36784741144415	5.7647838574269095	4.974740407982915	5.892628323146034
9	75.66683850062597	9.446940128139039	9.583327196406216	5.302894174828779
10	35.596730245231605	31.20730539804109	14.307386405479047	18.88857795124825
11	27.6851019957287	29.135576993887618	24.38913027468886	18.79019073569482
12	25.989248103689523	23.987627954930407	29.774799322483247	20.248324618896827
13	24.62184255099786	25.493777155902496	27.51955224979748	22.364828043302158
14	22.24110759260623	27.914868547021136	27.987627954930407	21.856395905442227
15	20.358200162014874	29.383017895279472	29.12880182634951	21.129980116356137
16	21.595110096472496	27.305103468591206	26.477649311436778	24.62213712349952
17	20.783268281905883	27.309816628617718	28.138449075778777	23.76846601369762
18	21.696443037042492	27.337506443773474	27.017600706974005	23.94844981221003
19	22.816996833345605	30.18690625230135	25.7094042271154	21.286692687237647
20	23.592017085205097	28.36173503203476	25.34560718756904	22.700640695191105
21	23.029089034538625	29.585389203917817	24.940864570292362	22.444657191251196
22	21.540908756167614	27.564916415052654	26.331246778113265	24.56292805066647
23	23.302452316076295	28.143751380808602	24.11959643567273	24.434199867442373
24	20.58443184328743	28.97915899550777	25.401575962883864	25.03483319832094
25	22.643493629869653	28.045953310258486	24.24979748140511	25.060755578466747
26	22.634951027321602	27.803814713896458	24.741438986670595	24.819795272111346
27	20.240371161352087	26.067015244126964	26.76809779807055	26.9245157964504
28	23.854775756683114	26.67177259002872	27.01406583695412	22.459385816334045
29	23.09006554238162	27.430591354297075	23.52249797481405	25.956845128507254
30	23.15310405773621	27.139848295161645	26.73422196038	22.972825686722146
31	25.070771043523088	26.330952205611606	23.077693497312026	25.52058325355328
32	24.152293983356653	25.29287870977244	25.75624125487886	24.798586051992046
33	23.608513145297884	25.37742101774799	27.583179910155387	23.430885926798734
34	24.316370866779586	24.379409382134178	26.423742543633548	24.880477207452685
35	22.25848737020399	23.792915531335147	28.88666322998748	25.06193386847338
36	23.2182045806024	25.66786950438177	28.967081522939836	22.146844392076
37	22.273805140290154	25.62869136166139	28.2238751012593	23.873628396789158
38	22.676191177553576	24.42801384490758	30.22844097503498	22.667354002503867
39	22.723617350320346	23.00935267692761	29.134987848884307	25.132042123867738
40	24.69253995139554	24.43832388246557	27.844760291626773	23.024375874512117
41	22.340083953162974	22.880329921201856	29.809853450180423	24.969732675454743
42	24.19765814861183	23.151925767729583	27.739303336033583	24.91111274762501
43	21.60335812651889	23.032918477060164	28.97915899550777	26.384564400913174
44	23.744900213565064	22.091170189262833	27.804109286398116	26.35982031077399
45	24.445099050003684	22.71831504529052	29.334413432506075	23.50217247219972
46	22.480005891450034	24.92849252522277	29.041019220855734	23.550482362471463
47	23.60733485529126	23.0237867295088	28.380882244642464	24.987996170557476
48	22.46262611385227	22.51152514912733	30.18749539730466	24.838353339715738
49	21.660505191840343	22.760733485529126	29.902349215700713	25.67641210692982
50	22.288533765373003	22.002503866264085	28.251859488916708	27.457102879446204
51	22.622873554753664	21.936519625892924	30.507401134104136	24.933205685249284
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1495.0
1	1217.5
2	940.0
3	653.5
4	367.0
5	373.5
6	380.0
7	372.5
8	365.0
9	405.0
10	445.0
11	560.5
12	676.0
13	667.0
14	658.0
15	687.0
16	716.0
17	747.0
18	778.0
19	848.0
20	918.0
21	942.5
22	967.0
23	1020.5
24	1074.0
25	1673.0
26	2608.5
27	2945.0
28	3492.5
29	4040.0
30	4291.5
31	4543.0
32	5522.0
33	6501.0
34	7301.5
35	8102.0
36	8682.5
37	9263.0
38	9800.5
39	10338.0
40	11766.0
41	13194.0
42	14600.5
43	16007.0
44	19929.0
45	23851.0
46	26775.0
47	29699.0
48	32101.5
49	34504.0
50	32761.0
51	31018.0
52	27531.5
53	24045.0
54	22209.0
55	20373.0
56	18711.0
57	17049.0
58	16543.0
59	16037.0
60	14483.5
61	12930.0
62	12015.5
63	11101.0
64	9785.5
65	8470.0
66	7749.0
67	7028.0
68	6126.0
69	5224.0
70	4688.5
71	4153.0
72	3422.5
73	2692.0
74	2144.5
75	1411.5
76	1226.0
77	958.5
78	691.0
79	521.0
80	351.0
81	312.0
82	273.0
83	205.0
84	137.0
85	81.5
86	26.0
87	16.0
88	6.0
89	6.0
90	6.0
91	4.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	339475.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.526832508983127
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.30999999914411	22.827194212442137
2	9.702999999892578	5.729977116629829
3	3.5319999999608975	3.1286631726172147
4	1.681000001085321	1.9853842191858688
5	0.9489999999926441	1.4010482025403894
6	0.6839999999924389	1.2117812061552724
7	0.4249999999952949	0.8784232671325233
8	0.3369999999962691	0.7960434044333722
9	0.26599999999705515	0.7068723702572303
>10	3.6359999999597457	26.717649663782684
>50	1.3129999999854638	26.579464087642172
>100	0.15699999999826186	6.178784970761404
>500	0.004999999999944645	0.8539159961503383
>1k	0.001999999999977858	1.0047981102695718
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGCT	2222	0.6545400986817881	Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1181	0.34789012445688194	No Hit
AAACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCT	729	0.21474335370793135	No Hit
AATCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCT	574	0.16908461595110097	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTT	561	0.16525517342956034	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCC	524	0.15435599086825244	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGT	504	0.14846454083511304	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	484	0.14257309080197364	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	413	0.12165844318432874	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCT	356	0.10486781058988144	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	347	0.1022166580749687	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7234700640695191	0.0
2	0.0	0.0	0.0	1.0486781058988144	0.0
3	0.0	0.0	0.0	1.4124751454451727	0.0
4	0.0	0.0	0.0	3.0429339421165036	0.0
5	0.0	0.0	0.0	3.3012740260696662	0.0
6	0.0	0.0	0.0	4.711392591501584	0.0
7	0.0	0.0	0.0	5.8298843802931	0.0
8	0.0	0.0	0.0	6.89152367626482	0.0
9	0.0	0.0	0.0	8.125782458207526	0.0
10	0.0	0.0	0.0	10.133294056999778	0.0
11	0.0	0.0	0.0	11.618528610354224	0.0
12	0.0	0.0	0.0	12.405626334781648	0.0
13	0.0	0.0	0.0	12.75351645923853	0.0
14	0.0	0.0	0.0	13.122615803814714	0.0
15	0.0	0.0	0.0	13.415126297960086	0.0
16	0.0	0.0	0.0	13.998674423742544	0.0
17	0.0	0.0	0.0	14.689152367626482	0.0
18	0.0	0.0	0.0	15.718388688415937	0.0
19	0.0	0.0	0.0	16.06156565284631	0.0
20	0.0	0.0	0.0	16.5346490905074	0.0
21	0.0	0.0	0.0	16.955004050371898	0.0
22	0.0	0.0	0.0	17.39538994034907	0.0
23	0.0	0.0	0.0	17.852566462920688	0.0
24	0.0	0.0	0.0	18.183371382281464	0.0
25	0.0	0.0	0.0	18.46203696884896	0.0
26	0.0	0.0	0.0	18.72921422785183	0.0
27	0.0	0.0	0.0	19.036158774578393	0.0
28	0.0	0.0	0.0	19.336328153766846	0.0
29	0.0	0.0	0.0	19.608513145297888	0.0
30	0.0	0.0	0.0	20.074232270417557	0.0
31	0.0	0.0	0.0	20.354076146991677	0.0
32	0.0	0.0	0.0	20.644819206127107	0.0
33	0.0	0.0	0.0	20.940275425289048	0.0
34	0.0	0.0	0.0	21.204506959275353	0.0
35	0.0	0.0	0.0	21.496722880919066	0.0
36	0.0	0.0	0.0	21.777450474998158	0.0
37	0.0	0.0	0.0	22.0749687016717	0.0
38	0.0	0.0	0.0	22.366300905810444	0.0
39	0.0	0.0	0.0	22.649385079902792	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGATG	30	2.1609649E-6	45.000004	21
GGCGTGA	30	2.1609649E-6	45.000004	19
AAGGTAG	30	2.1609649E-6	45.000004	1
TGTGCCA	30	2.1609649E-6	45.000004	29
CACTCTA	30	2.1609649E-6	45.000004	44
CGGGCTA	30	2.1609649E-6	45.000004	6
GTATCTA	30	2.1609649E-6	45.000004	23
GCGTGAT	30	2.1609649E-6	45.000004	20
AACTACG	30	2.1609649E-6	45.000004	1
TATACGG	30	2.1609649E-6	45.000004	2
TTACCGG	30	2.1609649E-6	45.000004	2
CGATCCC	30	2.1609649E-6	45.000004	43
CAATACG	30	2.1609649E-6	45.000004	1
ACTATCC	25	3.884979E-5	45.0	35
ACTATAA	25	3.884979E-5	45.0	32
TGCCCGC	25	3.884979E-5	45.0	45
TCACCCG	25	3.884979E-5	45.0	15
ATTAGCG	35	1.2085547E-7	45.0	1
CTCGTAA	20	7.0262566E-4	45.0	33
CGAGTTA	20	7.0262566E-4	45.0	45
>>END_MODULE