FastQCFastQC Report
Sat 18 Jun 2016
SRR3553167_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553167_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344951
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGCT23820.690532858290018Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12790.37077729880475774No Hit
AAACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCT8570.24844108293641703No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTT6410.18582349377157917No Hit
AATCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCT6270.18176494632571005No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGT5760.16698023777290108No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCC5500.15944293537342985No Hit
CCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC4600.13335227322141407No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG4120.11943725340700564No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCT3480.10088389365446107No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAGT351.2086275E-745.00000416
TCGGGTT351.2086275E-745.00000435
GGATAGT351.2086275E-745.0000048
TGACGAT351.2086275E-745.00000428
ATGACGA351.2086275E-745.00000427
GACGATT351.2086275E-745.00000429
TACACGC351.2086275E-745.00000435
ACAACGC207.0264144E-445.038
TAGAGTC253.885111E-545.042
AAGTAGC207.0264144E-445.020
CTATGCG406.7902874E-945.01
CCTTAGG207.0264144E-445.02
CTGTCGA207.0264144E-445.012
CAAACTA207.0264144E-445.025
CGAACGT207.0264144E-445.037
CGAACCA207.0264144E-445.014
CGTGCTT253.885111E-545.030
CAACGAA207.0264144E-445.029
CGTGCAC207.0264144E-445.021
CGTTGTA253.885111E-545.033