##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553167_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 344951 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.713785436192385 31.0 31.0 34.0 30.0 34.0 2 31.91460236381399 33.0 31.0 34.0 30.0 34.0 3 32.082649419772665 34.0 31.0 34.0 30.0 34.0 4 35.76146757075642 37.0 35.0 37.0 35.0 37.0 5 35.52178715237817 37.0 35.0 37.0 33.0 37.0 6 35.54968966606852 37.0 35.0 37.0 33.0 37.0 7 35.611762250290624 37.0 35.0 37.0 35.0 37.0 8 35.66280138338489 37.0 35.0 37.0 35.0 37.0 9 37.341938420239394 39.0 37.0 39.0 34.0 39.0 10 36.915538728689 39.0 37.0 39.0 33.0 39.0 11 36.93081626086024 39.0 37.0 39.0 33.0 39.0 12 36.82713196946813 39.0 37.0 39.0 33.0 39.0 13 36.79659429890042 39.0 37.0 39.0 33.0 39.0 14 37.96420361152743 40.0 37.0 41.0 33.0 41.0 15 37.972656986064685 40.0 37.0 41.0 33.0 41.0 16 37.891459946485156 40.0 37.0 41.0 33.0 41.0 17 37.84535774646254 40.0 37.0 41.0 33.0 41.0 18 37.79592173960939 39.0 37.0 41.0 33.0 41.0 19 37.82156596154236 40.0 36.0 41.0 33.0 41.0 20 37.720690764775284 39.0 36.0 41.0 33.0 41.0 21 37.6928810178837 39.0 36.0 41.0 33.0 41.0 22 37.6594037993802 39.0 36.0 41.0 33.0 41.0 23 37.51728216471325 39.0 36.0 41.0 32.0 41.0 24 37.35682169351589 39.0 36.0 41.0 32.0 41.0 25 37.41514881823795 39.0 36.0 41.0 32.0 41.0 26 37.350058414093596 39.0 36.0 41.0 32.0 41.0 27 37.29682766537856 39.0 36.0 41.0 32.0 41.0 28 37.24424918321733 39.0 36.0 41.0 32.0 41.0 29 37.081895689532715 39.0 35.0 41.0 31.0 41.0 30 37.060118683523164 39.0 35.0 41.0 31.0 41.0 31 37.025455789372984 39.0 35.0 41.0 31.0 41.0 32 36.936950465428424 39.0 35.0 41.0 31.0 41.0 33 36.85161950537903 39.0 35.0 41.0 31.0 41.0 34 36.73338532139347 39.0 35.0 41.0 31.0 41.0 35 36.67008647605022 39.0 35.0 41.0 30.0 41.0 36 36.60107377569568 39.0 35.0 40.0 30.0 41.0 37 36.50157558609774 39.0 35.0 40.0 30.0 41.0 38 36.479146313534386 39.0 35.0 40.0 30.0 41.0 39 36.4172447680975 39.0 35.0 40.0 30.0 41.0 40 36.29027021229102 39.0 35.0 40.0 30.0 41.0 41 36.17826299967242 39.0 35.0 40.0 30.0 41.0 42 36.0796924780621 38.0 35.0 40.0 30.0 41.0 43 35.99478476653206 38.0 35.0 40.0 29.0 41.0 44 35.91306591370948 38.0 35.0 40.0 29.0 41.0 45 35.73023125023554 38.0 35.0 40.0 28.0 41.0 46 35.57602674002975 38.0 34.0 40.0 28.0 41.0 47 35.436737391687515 38.0 34.0 40.0 28.0 41.0 48 35.37507935909738 38.0 34.0 40.0 28.0 41.0 49 35.194479795681126 38.0 34.0 40.0 27.0 41.0 50 35.11123898756635 37.0 34.0 40.0 27.0 41.0 51 33.777493035242685 36.0 32.0 39.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 1.0 12 3.0 13 5.0 14 8.0 15 20.0 16 47.0 17 98.0 18 200.0 19 368.0 20 614.0 21 845.0 22 1159.0 23 1594.0 24 1909.0 25 2318.0 26 2663.0 27 3196.0 28 3754.0 29 4933.0 30 6332.0 31 8172.0 32 10665.0 33 14390.0 34 22055.0 35 28195.0 36 32511.0 37 47180.0 38 71165.0 39 80539.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.78874970648005 33.08208992001763 20.807882858724863 25.321277514777464 2 22.377960927783946 32.506935767688745 20.500882734069474 24.614220570457835 3 21.03255244947833 32.21385066284776 21.11430319088798 25.63929369678592 4 20.830494765923277 31.951494560096943 19.78136025116611 27.436650422813674 5 19.036037002356856 34.25124148067407 18.510455108116805 28.20226640885227 6 20.749323817005894 37.903064493217876 19.74541311664555 21.602198573130675 7 81.52665161138827 7.295528930195882 6.180298071320275 4.997521387095558 8 82.9230818290134 5.844598218297671 5.153485567515386 6.078834385173546 9 75.42288614904726 9.462793266289994 9.788056854451792 5.3262637302109574 10 35.370240990749416 31.191676498981014 14.391029450559644 19.047053059709928 11 27.608558896770845 29.12355667906456 24.522033564187375 18.745850859977214 12 25.8277262567727 23.752069134456775 29.942513574391725 20.477691034378793 13 24.485216740928422 25.33780160080707 27.637548521384197 22.53943313688031 14 21.999646326579718 27.93933051360918 28.26314462054031 21.797878539270794 15 20.21272586541277 29.294595464283336 29.313728616528145 21.178950053775754 16 21.408547880713495 27.358668332603763 26.76670019799914 24.46608358868361 17 20.681777991656787 27.29923960214639 28.19878765389867 23.82019475229815 18 21.493777377076746 27.26996008128691 27.17545390504738 24.060808636588966 19 22.49218004876055 30.361703546300777 25.875849033630864 21.270267371307806 20 23.421877310110713 28.528109789506335 25.360993300497753 22.689019599885203 21 22.798310484677533 29.56622824691043 25.2134361112158 22.42202515719624 22 21.394632860899083 27.629431426492456 26.28779159938658 24.688144113221878 23 23.290844206858367 28.148635603317572 23.99326281123986 24.567257378584202 24 20.466385080779588 29.07949244965227 25.39404147255697 25.06008099701117 25 22.582917573800337 27.915559021426233 24.348385712753405 25.153137692020028 26 22.723227356928955 27.772930068328545 24.843818397395573 24.66002417734693 27 20.144600247571397 25.942235273995433 26.91135842481976 27.001806053613414 28 23.76946290922479 26.551017390875803 27.125301854466286 22.55421784543312 29 23.04907073758302 27.313734414453066 23.504497740258763 26.132697107705155 30 23.145896083791612 26.963829645369923 26.90845946235842 22.981814808480046 31 25.0586315157805 26.314462054030862 23.02877800035367 25.59812842983496 32 24.021092850868676 25.320697722285196 25.814680925696692 24.84352850114944 33 23.676116317969797 25.27518401164223 27.670596693443418 23.37810297694455 34 24.365779487521415 24.330412145493128 26.405779371562915 24.89802899542254 35 22.188658679058765 23.739893492119172 29.032239361532508 25.03920846728956 36 23.331719577563188 25.42390078590872 29.089638818266945 22.154740818261146 37 22.329838150925784 25.567399427744807 28.394757516284923 23.708004905044486 38 22.83744647790556 24.239094827961072 30.30401419332021 22.61944450081316 39 22.62553232198196 22.993990450817652 29.252270612347843 25.128206614852544 40 24.66350293230053 24.383753054781693 27.93440227742491 23.018341735492868 41 22.281135581575352 22.61944450081316 30.075575951367007 25.02384396624448 42 24.398827659580636 23.169377679728427 27.74538992494586 24.686404735745075 43 21.572919052271192 22.767001690095114 29.19371157062887 26.466367687004823 44 23.741342973349838 21.985151514273042 27.75118784986853 26.522317662508588 45 24.348965505245673 22.558566289125125 29.616960089983795 23.47550811564541 46 22.361146945508203 24.66901096097707 29.260097810993447 23.709744282521285 47 23.632921777295905 22.962101863742966 28.415050253514266 24.989926105446862 48 22.43101194082638 22.275627552898815 30.465486402416573 24.827874103858232 49 21.76598995219611 22.479714510176805 30.007450333525632 25.746845204101454 50 22.301138422558566 21.71989644906088 28.465492200341497 27.513472928039057 51 22.666117796440655 21.68423921078646 30.62290006406707 25.026742928705815 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1565.0 1 1237.5 2 910.0 3 644.0 4 378.0 5 380.0 6 382.0 7 390.0 8 398.0 9 447.0 10 496.0 11 589.0 12 682.0 13 650.0 14 618.0 15 673.0 16 728.0 17 774.5 18 821.0 19 848.5 20 876.0 21 968.5 22 1061.0 23 1112.5 24 1164.0 25 1738.0 26 2576.0 27 2840.0 28 3497.5 29 4155.0 30 4343.0 31 4531.0 32 5506.0 33 6481.0 34 7348.0 35 8215.0 36 8716.0 37 9217.0 38 9791.5 39 10366.0 40 11885.5 41 13405.0 42 14855.0 43 16305.0 44 20673.5 45 25042.0 46 27763.0 47 30484.0 48 33038.5 49 35593.0 50 33543.0 51 31493.0 52 27833.0 53 24173.0 54 22361.5 55 20550.0 56 19023.0 57 17496.0 58 16723.0 59 15950.0 60 14539.0 61 13128.0 62 12016.0 63 10904.0 64 9724.0 65 8544.0 66 7839.5 67 7135.0 68 6213.0 69 5291.0 70 4658.0 71 4025.0 72 3408.5 73 2792.0 74 2180.0 75 1449.5 76 1331.0 77 1024.5 78 718.0 79 550.5 80 383.0 81 327.5 82 272.0 83 205.5 84 139.0 85 77.5 86 16.0 87 12.5 88 9.0 89 7.0 90 5.0 91 3.5 92 2.0 93 1.5 94 1.0 95 0.5 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 344951.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.04924718842547 #Duplication Level Percentage of deduplicated Percentage of total 1 77.0759999945808 22.389997761376577 2 9.74199999931504 5.659955321794867 3 3.603999999746603 3.140804605791732 4 1.689000006878989 1.9625671480432028 5 0.9959999999630487 1.4466525099299181 6 0.6459999999547517 1.1259488209445054 7 0.4589999999677287 0.9333523120984883 8 0.35499999997504 0.8249986200932776 9 0.26899999998108665 0.7032822743823328 >10 3.6629999997424547 26.634964252722725 >50 1.3279999999066285 26.63815966991323 >100 0.16599999998832857 6.531432737386359 >500 0.004999999999648451 0.944391026029312 >1k 0.0019999999998593803 1.0634929394934824 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGCT 2382 0.690532858290018 Illumina Single End Adapter 1 (95% over 21bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1279 0.37077729880475774 No Hit AAACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCT 857 0.24844108293641703 No Hit AAAAACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTT 641 0.18582349377157917 No Hit AATCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCT 627 0.18176494632571005 No Hit AATGATACCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGT 576 0.16698023777290108 No Hit AATGATACGGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCC 550 0.15944293537342985 No Hit CCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC 460 0.13335227322141407 No Hit TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG 412 0.11943725340700564 No Hit AAAAAACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCT 348 0.10088389365446107 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.7760522508994031 0.0 2 0.0 0.0 0.0 1.102475424045734 0.0 3 0.0 0.0 0.0 1.5083301686326465 0.0 4 0.0 0.0 0.0 3.238430965557427 0.0 5 0.0 0.0 0.0 3.505135512000255 0.0 6 0.0 0.0 0.0 5.015205058109703 0.0 7 0.0 0.0 0.0 6.150148861722389 0.0 8 0.0 0.0 0.0 7.2801644291508065 0.0 9 0.0 0.0 0.0 8.579769300567326 0.0 10 0.0 0.0 0.0 10.63890233685364 0.0 11 0.0 0.0 0.0 12.224055010711666 0.0 12 0.0 0.0 0.0 13.030836263701222 0.0 13 0.0 0.0 0.0 13.390307608906772 0.0 14 0.0 0.0 0.0 13.7651434551574 0.0 15 0.0 0.0 0.0 14.05677907876771 0.0 16 0.0 0.0 0.0 14.643529080941931 0.0 17 0.0 0.0 0.0 15.331742769262881 0.0 18 0.0 0.0 0.0 16.402909398726194 0.0 19 0.0 0.0 0.0 16.773686697530955 0.0 20 0.0 0.0 0.0 17.263901249742716 0.0 21 0.0 0.0 0.0 17.676133711744566 0.0 22 0.0 0.0 0.0 18.140837394296582 0.0 23 0.0 0.0 0.0 18.62554391783181 0.0 24 0.0 0.0 0.0 18.98037692309922 0.0 25 0.0 0.0 0.0 19.256648045664456 0.0 26 0.0 0.0 0.0 19.5485735655209 0.0 27 0.0 0.0 0.0 19.86832912500616 0.0 28 0.0 0.0 0.0 20.173010079692478 0.0 29 0.0 0.0 0.0 20.47479207191746 0.0 30 0.0 0.0 0.0 20.91514446979426 0.0 31 0.0 0.0 0.0 21.218665839496044 0.0 32 0.0 0.0 0.0 21.53291337030477 0.0 33 0.0 0.0 0.0 21.83063681508388 0.0 34 0.0 0.0 0.0 22.102849390203247 0.0 35 0.0 0.0 0.0 22.39999304249009 0.0 36 0.0 0.0 0.0 22.662059248994783 0.0 37 0.0 0.0 0.0 22.920936596792007 0.0 38 0.0 0.0 0.0 23.208223776710316 0.0 39 0.0 0.0 0.0 23.488263550475285 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCTAGT 35 1.2086275E-7 45.000004 16 TCGGGTT 35 1.2086275E-7 45.000004 35 GGATAGT 35 1.2086275E-7 45.000004 8 TGACGAT 35 1.2086275E-7 45.000004 28 ATGACGA 35 1.2086275E-7 45.000004 27 GACGATT 35 1.2086275E-7 45.000004 29 TACACGC 35 1.2086275E-7 45.000004 35 ACAACGC 20 7.0264144E-4 45.0 38 TAGAGTC 25 3.885111E-5 45.0 42 AAGTAGC 20 7.0264144E-4 45.0 20 CTATGCG 40 6.7902874E-9 45.0 1 CCTTAGG 20 7.0264144E-4 45.0 2 CTGTCGA 20 7.0264144E-4 45.0 12 CAAACTA 20 7.0264144E-4 45.0 25 CGAACGT 20 7.0264144E-4 45.0 37 CGAACCA 20 7.0264144E-4 45.0 14 CGTGCTT 25 3.885111E-5 45.0 30 CAACGAA 20 7.0264144E-4 45.0 29 CGTGCAC 20 7.0264144E-4 45.0 21 CGTTGTA 25 3.885111E-5 45.0 33 >>END_MODULE