FastQCFastQC Report
Sat 18 Jun 2016
SRR3553160_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553160_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700939
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGCT39120.5581084801958516Illumina Single End Adapter 2 (95% over 21bp)
CTCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCCTTTCTGGTC13330.19017346730599954No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12380.1766202194484827No Hit
CTCCCAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTCTGG9410.13424848667287737No Hit
CCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC8720.12440454875531251No Hit
AAACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCT8550.12197923071765161No Hit
AGCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCCTTTCTGGTC7990.11398994777006273No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG7010.10000870261178219No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGC302.1642845E-645.00000414
GTCGAAA207.0314E-445.00000421
TAGGTCG207.0314E-445.00000437
CTATACG207.0314E-445.0000041
ATAGCGA207.0314E-445.00000437
CGATTCA207.0314E-445.00000424
TAACGGT302.1642845E-645.0000043
TATTCCG302.1642845E-645.0000041
ACTCGAA207.0314E-445.00000426
TTAGTCG406.8084773E-945.00000431
AATTACG406.8084773E-945.0000041
AACTCGA207.0314E-445.00000425
ATCGGCC302.1642845E-645.00000430
TAGTCGG207.0314E-445.0000042
CGTTCAC253.8892435E-545.030
GCGCACT253.8892435E-545.016
GCCGATA253.8892435E-545.024
ACGTGCG351.2110286E-745.01
TATAGCG253.8892435E-545.01
AATAGCG502.1827873E-1145.01