##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553160_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	700939
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72192872703616	31.0	31.0	34.0	30.0	34.0
2	31.923158791278556	33.0	31.0	34.0	30.0	34.0
3	32.114924408543395	34.0	31.0	34.0	30.0	34.0
4	35.78515248830497	37.0	35.0	37.0	35.0	37.0
5	35.557527830524485	37.0	35.0	37.0	33.0	37.0
6	34.90146646141818	37.0	35.0	37.0	32.0	37.0
7	35.36317282959002	37.0	35.0	37.0	33.0	37.0
8	35.74707071514069	37.0	35.0	37.0	35.0	37.0
9	37.34362619286414	39.0	37.0	39.0	35.0	39.0
10	36.910571390663094	39.0	37.0	39.0	33.0	39.0
11	36.91106358755898	39.0	37.0	39.0	33.0	39.0
12	36.84130573416517	39.0	37.0	39.0	33.0	39.0
13	36.79204467150494	39.0	37.0	39.0	33.0	39.0
14	37.94384960745514	40.0	37.0	41.0	33.0	41.0
15	37.97509341041089	40.0	37.0	41.0	33.0	41.0
16	37.88528673679165	40.0	37.0	41.0	33.0	41.0
17	37.85827154716744	40.0	37.0	41.0	33.0	41.0
18	37.85583909584143	40.0	37.0	41.0	33.0	41.0
19	37.86312646321577	40.0	37.0	41.0	33.0	41.0
20	37.7498041912349	40.0	36.0	41.0	33.0	41.0
21	37.66451574245405	39.0	36.0	41.0	32.0	41.0
22	37.6411913162201	39.0	36.0	41.0	32.0	41.0
23	37.55167996073838	39.0	36.0	41.0	32.0	41.0
24	37.42939114530651	39.0	36.0	41.0	32.0	41.0
25	37.38626613728156	39.0	36.0	41.0	32.0	41.0
26	37.40235598247494	39.0	36.0	41.0	32.0	41.0
27	37.297132846082185	39.0	36.0	41.0	32.0	41.0
28	37.14462171458572	39.0	36.0	41.0	31.0	41.0
29	36.898745825243	39.0	35.0	41.0	31.0	41.0
30	36.99088651080907	39.0	35.0	41.0	31.0	41.0
31	36.944321831143654	39.0	35.0	41.0	31.0	41.0
32	36.93322956776552	39.0	35.0	41.0	31.0	41.0
33	36.72104847925426	39.0	35.0	41.0	30.0	41.0
34	36.727351452836835	39.0	35.0	41.0	30.0	41.0
35	36.6685217971892	39.0	35.0	41.0	30.0	41.0
36	36.59435271828219	39.0	35.0	40.0	30.0	41.0
37	36.52410266799251	39.0	35.0	40.0	30.0	41.0
38	36.335267405580225	39.0	35.0	40.0	30.0	41.0
39	36.327619093815585	39.0	35.0	40.0	30.0	41.0
40	36.23056785255208	38.0	35.0	40.0	30.0	41.0
41	36.17932944236232	38.0	35.0	40.0	30.0	41.0
42	36.055304384547014	38.0	35.0	40.0	30.0	41.0
43	36.01591293964239	38.0	35.0	40.0	30.0	41.0
44	35.95611030346435	38.0	35.0	40.0	30.0	41.0
45	35.75592740595116	38.0	35.0	40.0	29.0	41.0
46	35.625284083208385	38.0	34.0	40.0	29.0	41.0
47	35.490223828321724	38.0	34.0	40.0	28.0	41.0
48	35.3772653540465	38.0	34.0	40.0	28.0	41.0
49	35.287032109784164	37.0	34.0	40.0	28.0	41.0
50	35.18026818310866	37.0	34.0	40.0	27.0	41.0
51	34.018522296519386	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	2.0
11	10.0
12	13.0
13	11.0
14	23.0
15	37.0
16	76.0
17	142.0
18	289.0
19	549.0
20	1066.0
21	1869.0
22	2632.0
23	3251.0
24	3717.0
25	4262.0
26	5189.0
27	6169.0
28	7923.0
29	9991.0
30	13158.0
31	17163.0
32	22359.0
33	30333.0
34	46129.0
35	55880.0
36	67592.0
37	97756.0
38	143672.0
39	159638.0
40	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.682113564803785	30.860888037332778	20.207321892489933	28.249676505373504
2	23.54684216458208	29.29684323457533	21.617002335438603	25.53931226540398
3	21.005964855714975	28.365093110812783	20.61548865165157	30.01345338182067
4	19.845236176043848	30.300924902166948	19.07740901847379	30.77642990331541
5	18.13067898918451	32.537638795957996	17.984303912323327	31.347378302534175
6	19.471737198244067	38.87513749413287	18.903927445897576	22.749197861725484
7	84.83163299516792	5.013988378446626	5.562252920724913	4.59212570566055
8	86.53306493147049	4.139732558753329	4.424208097994263	4.9029944117819095
9	79.37580873656623	7.281090080591891	8.610164365230071	4.732936817611804
10	36.315713635566006	33.2298530970598	13.659819185407004	16.79461408196719
11	27.73279843181789	30.324607419475875	23.378924556915795	18.56366959179044
12	26.976812532902294	22.793709581004908	29.860515679681114	20.368962206411684
13	27.23246958722514	23.405745721096984	27.163990019102947	22.19779467257493
14	20.469684237858075	28.283202960599997	28.14324784325027	23.103864958291666
15	20.240420350415654	27.08794916533393	29.757653661730902	22.913976822519505
16	22.81910408751689	28.115428018700626	25.107034991632656	23.95843290214983
17	21.130084072936448	26.2907328597781	27.979324877057778	24.599858190227682
18	25.46141675666499	25.052964665969508	25.562424119645215	23.923194457720285
19	24.28770549220403	28.387348970452493	24.942541362372474	22.382404174971004
20	24.952527966056962	26.843848038131707	24.383291556041254	23.82033243977008
21	25.81323053789274	27.285398586752912	25.01615689810383	21.885213977250515
22	23.381635206487296	26.14107647027773	24.825412767730146	25.651875555504827
23	23.384345856058804	25.904679294489245	24.36659966131147	26.34437518814048
24	20.759723742008934	26.379756298336943	26.27575295425137	26.584767005402753
25	22.77444970247054	26.895778377291034	24.26002833342131	26.06974358681711
26	24.040037720828774	25.9614602697239	24.10295332404104	25.89554868540629
27	21.800042514398545	24.88718704480704	25.542736243810086	27.770034196984334
28	22.796277564809493	26.02637319367306	26.53597531311569	24.64137392840176
29	23.54969547992051	26.831008119108795	25.060811283150176	24.558485117820524
30	23.709481138872285	24.521106686887162	26.8223055073266	24.947106666913953
31	24.517968040014896	26.07288223368938	24.401267442673326	25.007882283622397
32	25.288363181389535	23.539423544702178	25.261399351441423	25.910813922466865
33	22.913263493684898	24.189694110329143	27.429348345576432	25.467694050409523
34	22.335181806120076	23.341118128681668	28.08204422924106	26.241655835957197
35	22.99415498352924	23.056499923673815	28.941177477640707	25.00816761515624
36	23.06976783999749	25.16795327410802	29.245055561182927	22.517223324711566
37	24.38543154254507	24.968078534651376	26.61758013179464	24.028909791008918
38	24.483585590186877	24.86578717976885	27.60026193434807	23.050365295696203
39	24.361463693702305	22.017037145885734	27.708117254140518	25.913381906271443
40	26.558231172755402	23.092166365404122	26.224963941227408	24.12463852061306
41	24.26231098569205	22.08394739057179	27.441046938463977	26.212694685272186
42	25.705660549634135	23.21600025109175	26.29472750125189	24.783611698022224
43	22.56173504399099	22.385257490309428	28.831895500179044	26.22111196552054
44	25.051680674067217	20.897966870155603	26.25392509191242	27.796427363864755
45	24.01293122511374	22.086515374376372	28.650852641956003	25.249700758553885
46	22.339889776428475	22.618516019225638	29.389433317307212	25.652160887038672
47	23.603765805583656	23.68722527923257	27.933671831642982	24.775337083540794
48	23.721037065992903	22.455449047634673	29.446071626774938	24.37744225959748
49	24.0986733510334	22.097785969963148	28.11300270066297	25.690537978340483
50	22.452453066529326	21.933720338003734	28.110720048392228	27.503106547074708
51	23.521590323837025	20.960739807600948	28.321580051901808	27.196089816660223
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1747.0
1	1402.5
2	1058.0
3	768.5
4	479.0
5	452.5
6	426.0
7	469.0
8	512.0
9	568.5
10	625.0
11	717.5
12	810.0
13	970.0
14	1130.0
15	978.5
16	827.0
17	1027.0
18	1227.0
19	1331.5
20	1436.0
21	1464.5
22	1493.0
23	2053.0
24	2613.0
25	2876.0
26	3516.5
27	3894.0
28	4761.5
29	5629.0
30	7063.0
31	8497.0
32	9603.5
33	10710.0
34	11811.5
35	12913.0
36	16267.0
37	19621.0
38	20038.5
39	20456.0
40	22228.5
41	24001.0
42	27167.0
43	30333.0
44	35592.5
45	40852.0
46	47495.0
47	54138.0
48	62199.5
49	70261.0
50	68108.5
51	65956.0
52	60370.0
53	54784.0
54	50492.0
55	46200.0
56	41706.5
57	37213.0
58	36305.5
59	35398.0
60	34042.5
61	32687.0
62	29805.0
63	26923.0
64	24640.5
65	22358.0
66	20051.5
67	17745.0
68	15161.0
69	12577.0
70	10947.0
71	9317.0
72	8377.0
73	7437.0
74	6120.5
75	4032.5
76	3261.0
77	2846.0
78	2431.0
79	1945.0
80	1459.0
81	1121.0
82	783.0
83	523.0
84	263.0
85	233.5
86	204.0
87	179.0
88	154.0
89	125.0
90	96.0
91	72.0
92	48.0
93	30.0
94	12.0
95	6.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	700939.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.628010606302748
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.54254248884149	20.115476226199473
2	10.292829851349266	5.481551649011956
3	3.755986756343135	3.000433655551129
4	1.804373248798255	1.9218748002691552
5	1.058404172585693	1.409159876668346
6	0.6965236045526219	1.112822275774046
7	0.5386489629280136	1.0040205208544786
8	0.37742588899050156	0.8040080460105854
9	0.28929851377822835	0.6933099503946857
>10	3.9191836372045175	25.88796233121341
>50	1.4755747026287538	27.548518159222034
>100	0.24155704391181737	8.978134903109792
>500	0.006011600640339346	1.0992921802105742
>1k	0.0016395274473652765	0.9434354255103459
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGCT	3912	0.5581084801958516	Illumina Single End Adapter 2 (95% over 21bp)
CTCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCCTTTCTGGTC	1333	0.19017346730599954	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1238	0.1766202194484827	No Hit
CTCCCAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTCTGG	941	0.13424848667287737	No Hit
CCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	872	0.12440454875531251	No Hit
AAACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCT	855	0.12197923071765161	No Hit
AGCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCCTTTCTGGTC	799	0.11398994777006273	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	701	0.10000870261178219	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4266576692122996E-4	0.0	0.0	0.6131774662274463	0.0
2	1.4266576692122996E-4	0.0	0.0	0.8947996901299542	0.0
3	1.4266576692122996E-4	0.0	0.0	1.2149416711011942	0.0
4	1.4266576692122996E-4	0.0	0.0	2.5658438180783207	0.0
5	1.4266576692122996E-4	0.0	0.0	2.78383710993396	0.0
6	1.4266576692122996E-4	0.0	0.0	4.055845087803646	0.0
7	1.4266576692122996E-4	0.0	0.0	4.953640759038946	0.0
8	1.4266576692122996E-4	0.0	0.0	5.821048621920024	0.0
9	1.4266576692122996E-4	0.0	0.0	6.843819504978322	0.0
10	1.4266576692122996E-4	0.0	0.0	8.71559436698486	0.0
11	1.4266576692122996E-4	0.0	0.0	10.032684727201653	0.0
12	1.4266576692122996E-4	0.0	0.0	10.832326350795148	0.0
13	1.4266576692122996E-4	0.0	0.0	11.189704096932829	0.0
14	1.4266576692122996E-4	0.0	0.0	11.508276754467936	0.0
15	1.4266576692122996E-4	0.0	0.0	11.789898978370443	0.0
16	1.4266576692122996E-4	0.0	0.0	12.43317892141827	0.0
17	1.4266576692122996E-4	0.0	0.0	13.03779644163044	0.0
18	1.4266576692122996E-4	0.0	0.0	14.239755527941805	0.0
19	2.853315338424599E-4	0.0	0.0	14.58728933616192	0.0
20	2.853315338424599E-4	0.0	0.0	15.293484882422009	0.0
21	2.853315338424599E-4	0.0	0.0	15.739886067118537	0.0
22	2.853315338424599E-4	0.0	0.0	16.218815046673107	0.0
23	2.853315338424599E-4	0.0	0.0	16.66692822057269	0.0
24	2.853315338424599E-4	0.0	0.0	17.0137486999582	0.0
25	2.853315338424599E-4	0.0	0.0	17.318054780801184	0.0
26	2.853315338424599E-4	0.0	0.0	17.567719872913337	0.0
27	2.853315338424599E-4	0.0	0.0	17.962048052683613	0.0
28	2.853315338424599E-4	0.0	0.0	18.27020610923347	0.0
29	2.853315338424599E-4	0.0	0.0	18.59719604701693	0.0
30	2.853315338424599E-4	0.0	0.0	19.218505461958888	0.0
31	2.853315338424599E-4	0.0	0.0	19.52295420856879	0.0
32	2.853315338424599E-4	0.0	0.0	19.84766149408151	0.0
33	2.853315338424599E-4	0.0	0.0	20.166662148917382	0.0
34	2.853315338424599E-4	0.0	0.0	20.463264278346617	0.0
35	2.853315338424599E-4	0.0	0.0	20.80095414864917	0.0
36	2.853315338424599E-4	0.0	0.0	21.09926826728146	0.0
37	2.853315338424599E-4	0.0	0.0	21.40771165536516	0.0
38	2.853315338424599E-4	0.0	0.0	21.711161741606617	0.0
39	2.853315338424599E-4	0.0	0.0	22.02345710539719	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGC	30	2.1642845E-6	45.000004	14
GTCGAAA	20	7.0314E-4	45.000004	21
TAGGTCG	20	7.0314E-4	45.000004	37
CTATACG	20	7.0314E-4	45.000004	1
ATAGCGA	20	7.0314E-4	45.000004	37
CGATTCA	20	7.0314E-4	45.000004	24
TAACGGT	30	2.1642845E-6	45.000004	3
TATTCCG	30	2.1642845E-6	45.000004	1
ACTCGAA	20	7.0314E-4	45.000004	26
TTAGTCG	40	6.8084773E-9	45.000004	31
AATTACG	40	6.8084773E-9	45.000004	1
AACTCGA	20	7.0314E-4	45.000004	25
ATCGGCC	30	2.1642845E-6	45.000004	30
TAGTCGG	20	7.0314E-4	45.000004	2
CGTTCAC	25	3.8892435E-5	45.0	30
GCGCACT	25	3.8892435E-5	45.0	16
GCCGATA	25	3.8892435E-5	45.0	24
ACGTGCG	35	1.2110286E-7	45.0	1
TATAGCG	25	3.8892435E-5	45.0	1
AATAGCG	50	2.1827873E-11	45.0	1
>>END_MODULE