FastQCFastQC Report
Sat 18 Jun 2016
SRR3553157_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553157_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1021807
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCTTCTGCT36330.35554659539423783No Hit
AATCTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCTTCT31590.3091581874072109No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCC18220.17831155981511185No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGT18160.17772436477730139No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTACG406.8139343E-945.0000041
ACGCATG659.094947E-1238.0769271
AGGGCAT11850.036.4556966
TAGGGCC4050.036.111115
GACGGTC250.002106999536.035
TGCGGGC5950.035.924374
AACGGGA8650.035.8959544
TAAGGGC6400.035.859384
TTACGGG4100.035.670733
CTAACGG2400.035.6250042
ACGGGAC4750.035.5263145
CTAGGGC6550.035.381684
TAACGGG6500.035.3076933
AGGGCGT3700.035.270276
TCACGCG900.035.01
CAGGGCA9200.034.9728245
ACGGGCT9150.034.9180345
ACGGGGT8250.034.9090925
TACGGGC4900.034.897964
CGAGGGC4000.034.8754
Produced by FastQC (version 0.11.5)