FastQCFastQC Report
Sat 18 Jun 2016
SRR3553156_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553156_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932851
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGCT34820.37326432624288336TruSeq Adapter, Index 1 (95% over 24bp)
AATCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCT14630.15683104804518622Illumina Paired End PCR Primer 2 (95% over 22bp)
AATGATACCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGT10390.11137898764111309No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCC9830.10537588532359402No Hit
CCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC9570.10258873067617444Illumina Paired End PCR Primer 2 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCC4550.042.0329675
CGCACCG451.927583E-840.0000041
TCGTGCG403.4572804E-739.3751
CAGGGTA4100.038.9634135
TTGGCGT301.1397569E-437.5000041
CCACGCG1200.037.5000041
CGCCAGT301.1397569E-437.50000426
CGGGAAT10050.037.3880586
TAGTAGG1450.037.2413832
CGTACGG850.037.0588232
CGAGGGA3900.036.9230774
TACAGGG8250.036.818183
CTTGCGG1900.036.7105262
CAAGCGG2900.036.465522
AGGGTAT5750.036.3913046
ATGCGGG3900.036.3461533
TACCGGG5450.036.3302763
ACGGGCC4650.036.2903255
AAGGGCC5650.036.2389375
ACGGGAC4550.036.09895
Produced by FastQC (version 0.11.5)