##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553156_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	932851
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.774234041663675	31.0	31.0	34.0	30.0	34.0
2	31.97603154201475	33.0	31.0	34.0	30.0	34.0
3	32.146319187094186	34.0	31.0	34.0	30.0	34.0
4	35.810962308021324	37.0	35.0	37.0	35.0	37.0
5	35.57967242357032	37.0	35.0	37.0	33.0	37.0
6	34.88744611947674	37.0	35.0	37.0	33.0	37.0
7	35.404780613409855	37.0	35.0	37.0	33.0	37.0
8	35.842155928438736	37.0	35.0	37.0	35.0	37.0
9	37.49418074269096	39.0	37.0	39.0	35.0	39.0
10	37.04553781900861	39.0	37.0	39.0	33.0	39.0
11	37.005794065718966	39.0	37.0	39.0	33.0	39.0
12	36.95320581743494	39.0	37.0	39.0	33.0	39.0
13	36.96168627144099	39.0	37.0	39.0	33.0	39.0
14	38.15877455242048	40.0	37.0	41.0	33.0	41.0
15	38.14101608938619	40.0	37.0	41.0	33.0	41.0
16	38.03921526588919	40.0	37.0	41.0	33.0	41.0
17	38.02657873551082	40.0	37.0	41.0	33.0	41.0
18	38.001842738014965	40.0	37.0	41.0	33.0	41.0
19	38.057953520980305	40.0	37.0	41.0	33.0	41.0
20	37.9613121495287	40.0	37.0	41.0	33.0	41.0
21	37.962695007026845	40.0	37.0	41.0	33.0	41.0
22	37.94377987481388	40.0	37.0	41.0	33.0	41.0
23	37.87436686030245	40.0	37.0	41.0	33.0	41.0
24	37.75796134645297	40.0	37.0	41.0	33.0	41.0
25	37.8001020527394	40.0	37.0	41.0	33.0	41.0
26	37.76496139254822	40.0	37.0	41.0	33.0	41.0
27	37.717814527722005	40.0	37.0	41.0	33.0	41.0
28	37.66813028018408	40.0	37.0	41.0	33.0	41.0
29	37.46854427984748	40.0	36.0	41.0	32.0	41.0
30	37.528349114703204	40.0	36.0	41.0	32.0	41.0
31	37.51520768054062	40.0	36.0	41.0	32.0	41.0
32	37.51378944761811	40.0	36.0	41.0	33.0	41.0
33	37.25843784269942	40.0	36.0	41.0	32.0	41.0
34	37.33356023630783	40.0	36.0	41.0	32.0	41.0
35	37.23542237720708	39.0	36.0	41.0	32.0	41.0
36	37.238753027010745	39.0	36.0	41.0	32.0	41.0
37	37.17898678352706	39.0	36.0	41.0	31.0	41.0
38	37.005370632609065	39.0	35.0	41.0	31.0	41.0
39	36.940454584922996	39.0	35.0	41.0	31.0	41.0
40	36.87747239376921	39.0	35.0	41.0	31.0	41.0
41	36.87871267758731	39.0	35.0	41.0	31.0	41.0
42	36.83590412616806	39.0	35.0	41.0	31.0	41.0
43	36.771488694335964	39.0	35.0	41.0	31.0	41.0
44	36.67938609702943	39.0	35.0	40.0	31.0	41.0
45	36.49292759508217	39.0	35.0	40.0	30.0	41.0
46	36.35909593279098	38.0	35.0	40.0	30.0	41.0
47	36.220374958058684	38.0	35.0	40.0	30.0	41.0
48	36.13755465771061	38.0	35.0	40.0	30.0	41.0
49	36.042261840315334	38.0	35.0	40.0	30.0	41.0
50	35.93692990627657	38.0	35.0	40.0	29.0	41.0
51	34.8361506821561	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	15.0
12	10.0
13	21.0
14	21.0
15	49.0
16	79.0
17	138.0
18	254.0
19	487.0
20	783.0
21	1323.0
22	1907.0
23	2532.0
24	3252.0
25	4017.0
26	4986.0
27	6684.0
28	8593.0
29	11270.0
30	14996.0
31	19967.0
32	26829.0
33	36365.0
34	54674.0
35	70044.0
36	86299.0
37	129242.0
38	201095.0
39	246844.0
40	68.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.10964237589926	31.016743295553095	21.942625349600313	26.930988978947333
2	21.278424957469092	28.649591413848512	22.640164399244895	27.431819229437497
3	20.55376474914	29.388401791926043	22.55922971621406	27.498603742719897
4	19.50890335112467	29.631956228808242	21.196525490137226	29.66261492992986
5	17.893640034689355	32.82121153324593	20.007375239990093	29.27777319207462
6	19.72876697350381	35.64352720852526	20.44892485509476	24.17878096287617
7	85.8617292579415	4.282141520993171	5.336757960274471	4.5193712607908445
8	88.60782697343949	2.7790075799886584	4.072890525925362	4.540274920646492
9	80.27820091311474	6.32909221301151	7.97705099742617	5.415655876447579
10	35.18311070042268	26.206114374106903	15.178737011591348	23.432037913879064
11	23.19952489733087	26.15187205673789	29.946368712688308	20.70223433324293
12	21.36525554456178	20.027528512056055	38.72322589566822	19.883990047713944
13	24.756257966170374	22.502629037220306	30.84576207775947	21.89535091884985
14	20.599431206055417	27.679768794802172	29.818481193674018	21.902318805468397
15	19.758782485091402	33.43470715044525	27.779570370830925	19.026939993632425
16	20.1777132682497	31.111935346588037	28.09248207913161	20.617869306030652
17	18.226490618544656	32.27128448165891	29.6660452741113	19.83617962568513
18	18.968302547780944	31.831878831667655	29.320116503064263	19.879702117487145
19	18.285771253930154	35.382285059457516	29.129196409716023	17.20274727689631
20	18.79281900324918	32.82121153324593	29.338447404783828	19.04752205872106
21	18.72185375799565	33.68104874197487	28.84780098858231	18.749296511447167
22	16.878043760471932	32.747030340322304	30.025588223628425	20.349337675577342
23	17.38487711327961	31.575353405849384	29.34380731756733	21.69596216330368
24	16.258866635722104	32.269998102590876	30.16880509320352	21.3023301684835
25	17.762107774982287	32.014223064562294	29.0950001661573	21.128668994298124
26	19.008716290168525	32.46327655756385	28.9357035582317	19.59230359403592
27	17.47771080268982	31.60022340116482	28.419007965902377	22.503057830242987
28	18.69462540105547	31.729182902735808	29.828021838428647	19.748169857780073
29	19.58490691439469	32.843937563447966	27.15546212632028	20.41569339583706
30	18.27451543708481	32.124208474879694	28.271824760867492	21.329451327168
31	18.882115150222276	32.36626213618252	27.14495669726462	21.606666016330582
32	20.133011595635317	30.122281050242748	28.685931622520638	21.058775731601294
33	18.353413353257917	30.291011104667305	29.613625327088677	21.7419502149861
34	18.155310976779788	28.687753992867027	31.38968602702897	21.767249003324217
35	18.762696293405913	29.415630148866217	31.000449160691257	20.821224397036612
36	18.277731384754908	30.579052817652553	30.574764887425754	20.56845091016679
37	18.300350216701275	30.735669469186398	29.38057631926213	21.583403994850194
38	19.140784541153945	30.97836632002324	30.263568351215792	19.617280787607026
39	18.258650095245653	31.722107817861588	29.861896487220356	20.157345599672404
40	18.58581917155044	31.726181351577047	30.625791257124664	19.06220821974785
41	16.770845504801947	29.65918458574842	33.6412781891213	19.928691720328327
42	18.089920040821095	30.139003978127267	31.789428322422335	19.9816476586293
43	17.092011478789217	29.06809340398413	32.128710801617835	21.71118431560882
44	19.070248088923098	28.630402926083587	29.685876951410243	22.613472033583072
45	19.600236264955498	29.148492095736618	30.41010836671666	20.841163272591228
46	17.72973390176995	29.480806688313564	31.545230696006115	21.244228713910367
47	18.078021034441726	30.170627463549916	30.516020243318604	21.235331258689758
48	18.65839239063902	28.493510753593014	32.64958712591829	20.198509729849675
49	18.961120264651054	28.202253092937674	30.349541352263117	22.48708529014816
50	18.043824790883004	28.28115100911078	30.032341713735633	23.642682486270584
51	18.430917692107315	27.828238378905095	31.62252063834417	22.118323290643417
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1142.0
1	965.5
2	789.0
3	588.0
4	387.0
5	541.0
6	695.0
7	721.5
8	748.0
9	870.0
10	992.0
11	1230.0
12	1468.0
13	1521.0
14	1574.0
15	1995.0
16	2416.0
17	2676.0
18	2936.0
19	3740.5
20	4545.0
21	5747.5
22	6950.0
23	9480.0
24	12010.0
25	14115.0
26	19934.5
27	23649.0
28	27293.0
29	30937.0
30	33841.0
31	36745.0
32	41534.5
33	46324.0
34	48194.5
35	50065.0
36	50905.0
37	51745.0
38	52470.0
39	53195.0
40	52659.0
41	52123.0
42	53847.0
43	55571.0
44	58969.0
45	62367.0
46	66604.5
47	70842.0
48	74351.0
49	77860.0
50	72992.0
51	68124.0
52	59246.0
53	50368.0
54	42303.0
55	34238.0
56	29510.0
57	24782.0
58	22812.5
59	20843.0
60	19761.5
61	18680.0
62	16815.0
63	14950.0
64	13350.0
65	11750.0
66	9903.0
67	8056.0
68	6570.0
69	5084.0
70	4439.5
71	3795.0
72	3235.0
73	2675.0
74	2347.5
75	1683.5
76	1347.0
77	1172.0
78	997.0
79	763.5
80	530.0
81	352.5
82	175.0
83	120.5
84	66.0
85	54.0
86	42.0
87	28.0
88	14.0
89	10.0
90	6.0
91	6.5
92	7.0
93	4.5
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	932851.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.069322490902486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.21938715668247	25.32684978293039
2	9.685914441596465	6.793569143432722
3	4.0917781017613075	4.304876574356404
4	2.440244508690613	3.4231088652770003
5	1.6924811432170381	2.967708351062481
6	1.3000600628841	2.735533536169528
7	1.0633889420957312	2.610463082053038
8	0.8525635140033284	2.3919059853247826
9	0.7111896429236596	2.2446845045891646
>10	5.657836371168059	38.63058822644126
>50	0.2515352014237024	5.496961097655501
>100	0.0314417802676339	2.047092956909976
>500	0.0012452190204798577	0.3733698422786683
>1k	9.339142653598933E-4	0.653288051519167
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGCT	3482	0.37326432624288336	TruSeq Adapter, Index 1 (95% over 24bp)
AATCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCT	1463	0.15683104804518622	Illumina Paired End PCR Primer 2 (95% over 22bp)
AATGATACCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGT	1039	0.11137898764111309	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCC	983	0.10537588532359402	No Hit
CCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	957	0.10258873067617444	Illumina Paired End PCR Primer 2 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.41818039536860657	0.0
2	0.0	0.0	0.0	0.6734194421188379	0.0
3	0.0	0.0	0.0	0.9684290417226331	0.0
4	0.0	0.0	0.0	2.0945467175358123	0.0
5	0.0	0.0	0.0	2.37240459623241	0.0
6	0.0	0.0	0.0	3.9034100837111176	0.0
7	0.0	0.0	0.0	5.098349039664426	0.0
8	0.0	0.0	0.0	6.357178155997046	0.0
9	0.0	0.0	0.0	7.656313816461578	0.0
10	0.0	0.0	0.0	9.562941991807909	0.0
11	0.0	0.0	0.0	11.156336864086548	0.0
12	1.0719825566998373E-4	0.0	0.0	11.945637620584638	0.0
13	1.0719825566998373E-4	0.0	0.0	12.32447625612236	0.0
14	1.0719825566998373E-4	0.0	0.0	12.623880984208625	0.0
15	1.0719825566998373E-4	0.0	0.0	12.85853796587022	0.0
16	1.0719825566998373E-4	0.0	0.0	13.331175075119178	0.0
17	1.0719825566998373E-4	0.0	0.0	13.992373916091637	0.0
18	1.0719825566998373E-4	0.0	0.0	14.806223073138154	0.0
19	1.0719825566998373E-4	0.0	0.0	15.204893385974824	0.0
20	1.0719825566998373E-4	0.0	0.0	15.637009554580528	0.0
21	1.0719825566998373E-4	0.0	0.0	16.056261932505834	0.0
22	1.0719825566998373E-4	0.0	0.0	16.55376903707023	0.0
23	1.0719825566998373E-4	0.0	0.0	17.046988211407825	0.0
24	1.0719825566998373E-4	0.0	0.0	17.45637834981149	0.0
25	1.0719825566998373E-4	0.0	0.0	17.818601255720367	0.0
26	1.0719825566998373E-4	0.0	0.0	18.13601529075919	0.0
27	1.0719825566998373E-4	0.0	0.0	18.53007607860205	0.0
28	1.0719825566998373E-4	1.0719825566998373E-4	0.0	18.892834975789274	0.0
29	1.0719825566998373E-4	1.0719825566998373E-4	0.0	19.276390334576476	0.0
30	1.0719825566998373E-4	1.0719825566998373E-4	0.0	19.883775651202605	0.0
31	1.0719825566998373E-4	1.0719825566998373E-4	0.0	20.252323254196007	0.0
32	1.0719825566998373E-4	1.0719825566998373E-4	0.0	20.621085253700752	0.0
33	1.0719825566998373E-4	1.0719825566998373E-4	0.0	20.980306608450867	0.0
34	1.0719825566998373E-4	1.0719825566998373E-4	0.0	21.330416111469034	0.0
35	1.0719825566998373E-4	1.0719825566998373E-4	0.0	21.68191919181091	0.0
36	1.0719825566998373E-4	1.0719825566998373E-4	0.0	22.054861923286783	0.0
37	1.0719825566998373E-4	1.0719825566998373E-4	0.0	22.41183211466783	0.0
38	1.0719825566998373E-4	1.0719825566998373E-4	0.0	22.770410279883926	0.0
39	1.0719825566998373E-4	1.0719825566998373E-4	0.0	23.12973883288971	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGGCC	455	0.0	42.032967	5
CGCACCG	45	1.927583E-8	40.000004	1
TCGTGCG	40	3.4572804E-7	39.375	1
CAGGGTA	410	0.0	38.963413	5
TTGGCGT	30	1.1397569E-4	37.500004	1
CCACGCG	120	0.0	37.500004	1
CGCCAGT	30	1.1397569E-4	37.500004	26
CGGGAAT	1005	0.0	37.388058	6
TAGTAGG	145	0.0	37.241383	2
CGTACGG	85	0.0	37.058823	2
CGAGGGA	390	0.0	36.923077	4
TACAGGG	825	0.0	36.81818	3
CTTGCGG	190	0.0	36.710526	2
CAAGCGG	290	0.0	36.46552	2
AGGGTAT	575	0.0	36.391304	6
ATGCGGG	390	0.0	36.346153	3
TACCGGG	545	0.0	36.330276	3
ACGGGCC	465	0.0	36.290325	5
AAGGGCC	565	0.0	36.238937	5
ACGGGAC	455	0.0	36.0989	5
>>END_MODULE