FastQCFastQC Report
Sat 18 Jun 2016
SRR3553154_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553154_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308423
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGCT46070.3521032571270912TruSeq Adapter, Index 13 (95% over 23bp)
AATCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCT38190.29187808529810316No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGT22940.1753255636747443No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCC22030.16837062631885866No Hit
CAGCTGGGGAATGATACGGCTGTCTCTTATACACATCTGACGCCGAGACTT15830.12098533883919804No Hit
CCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC15550.11884535811431012No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGCG403.4587174E-739.3751
CAGCTGG20500.039.073171
GGGAATG48650.037.415217
TTACGGG6950.037.2302173
CAGTACG850.037.0588261
TACGGGA7250.036.620694
TACGGGT3300.036.1363644
GGAATGA45500.035.9010968
ATAGGGC8100.035.8333324
ACTGGGC7750.035.709684
CTTTGCG702.1827873E-1135.3571431
ACAGGGC8950.035.195534
CCTATGG1800.035.0000042
TGCGGGC6050.034.958684
AGGGCTT14050.034.9110346
CTACTGG3550.034.8591542
AACGGGC7950.034.811324
ACGGGAC7200.034.6875045
ATAGGGA8500.034.6764684
TTTGCGG1950.034.6153832
Produced by FastQC (version 0.11.5)