##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553154_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1308423 Sequences flagged as poor quality 0 Sequence length 51 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.692143137196457 31.0 31.0 34.0 30.0 34.0 2 31.89884387541338 33.0 31.0 34.0 30.0 34.0 3 32.08158065090571 34.0 31.0 34.0 30.0 34.0 4 35.74531325114279 37.0 35.0 37.0 35.0 37.0 5 35.512656839569466 37.0 35.0 37.0 33.0 37.0 6 34.800154078612195 37.0 35.0 37.0 32.0 37.0 7 35.34633677335235 37.0 35.0 37.0 33.0 37.0 8 35.7932167196694 37.0 35.0 37.0 35.0 37.0 9 37.385566441433696 39.0 37.0 39.0 35.0 39.0 10 36.971473292658416 39.0 37.0 39.0 33.0 39.0 11 36.932929182687865 39.0 37.0 39.0 33.0 39.0 12 36.92935847199262 39.0 37.0 39.0 33.0 39.0 13 36.946679323124094 39.0 37.0 39.0 33.0 39.0 14 38.11556736621108 40.0 37.0 41.0 33.0 41.0 15 38.113972316292205 40.0 37.0 41.0 33.0 41.0 16 37.98870166605142 40.0 37.0 41.0 33.0 41.0 17 37.97325864800604 40.0 37.0 41.0 33.0 41.0 18 37.96022998678562 40.0 37.0 41.0 33.0 41.0 19 38.01778706121797 40.0 37.0 41.0 33.0 41.0 20 37.92133660138961 40.0 37.0 41.0 33.0 41.0 21 37.9155884603068 40.0 37.0 41.0 33.0 41.0 22 37.8859749484685 40.0 37.0 41.0 33.0 41.0 23 37.78050752700006 40.0 37.0 41.0 33.0 41.0 24 37.708160892922244 40.0 37.0 41.0 32.0 41.0 25 37.68974712306341 40.0 37.0 41.0 32.0 41.0 26 37.704642153187464 40.0 37.0 41.0 33.0 41.0 27 37.6150082962467 40.0 37.0 41.0 33.0 41.0 28 37.503127046834244 40.0 37.0 41.0 32.0 41.0 29 37.26727442119253 39.0 36.0 41.0 31.0 41.0 30 37.337234212483274 39.0 36.0 41.0 32.0 41.0 31 37.32193717169447 39.0 36.0 41.0 32.0 41.0 32 37.2562367063251 39.0 36.0 41.0 31.0 41.0 33 37.08842018215822 39.0 36.0 41.0 31.0 41.0 34 37.11529986862047 39.0 36.0 41.0 31.0 41.0 35 37.06557359508355 39.0 36.0 41.0 31.0 41.0 36 37.059727626310455 39.0 36.0 41.0 31.0 41.0 37 37.00020024105354 39.0 36.0 41.0 31.0 41.0 38 36.81217847744957 39.0 35.0 41.0 31.0 41.0 39 36.77837213194815 39.0 35.0 41.0 31.0 41.0 40 36.76482681823845 39.0 35.0 41.0 31.0 41.0 41 36.7558587704435 39.0 35.0 41.0 31.0 41.0 42 36.706792833815975 39.0 35.0 41.0 31.0 41.0 43 36.61083151243902 39.0 35.0 40.0 31.0 41.0 44 36.466118373033794 39.0 35.0 40.0 30.0 41.0 45 36.28438127425152 39.0 35.0 40.0 30.0 41.0 46 36.14379294769352 38.0 35.0 40.0 29.0 41.0 47 36.057638852267196 38.0 35.0 40.0 29.0 41.0 48 35.945344128007534 38.0 35.0 40.0 29.0 41.0 49 35.84994684440735 38.0 35.0 40.0 29.0 41.0 50 35.71822950223284 38.0 34.0 40.0 28.0 41.0 51 34.6242927554774 37.0 33.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 4.0 9 7.0 10 19.0 11 22.0 12 14.0 13 15.0 14 33.0 15 55.0 16 88.0 17 222.0 18 398.0 19 807.0 20 1478.0 21 2480.0 22 3383.0 23 4363.0 24 5322.0 25 6362.0 26 7924.0 27 10112.0 28 13247.0 29 17233.0 30 22544.0 31 30044.0 32 39075.0 33 52336.0 34 76443.0 35 97672.0 36 122258.0 37 179710.0 38 276175.0 39 338464.0 40 113.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.011800465140094 30.773839958484373 21.442836147025847 27.77152342934968 2 20.49497754166657 29.119099862964802 21.53233319805598 28.85358939731264 3 21.322385803367872 28.157637094425887 22.24983816395768 28.27013893824856 4 19.422082919667417 28.35986527292779 21.03730979965959 31.1807420077452 5 17.78301054016935 32.8121715989401 21.232277329273483 28.17254053161707 6 21.016444987591935 34.882602950269145 20.67718161481417 23.423770447324756 7 85.54488877068043 4.3450015782357845 5.3721158982989445 4.737993752784841 8 87.98568964318115 2.7050120641413367 4.114342227246082 5.194956065431439 9 79.43952376257525 6.147018204357459 8.496029189337087 5.9174288437302005 10 34.13957107143485 26.10363773794866 15.749493856344623 24.00729733427187 11 21.331404293565612 27.21298846015394 30.953216199959797 20.502391046320646 12 19.014645875225366 20.107258891046705 41.20036104531944 19.67773418840849 13 24.329823000665687 23.346425429696666 31.35591471565388 20.967836853983766 14 19.90709426538665 29.75100559987099 29.336995757488214 21.004904377254142 15 19.378289742690246 33.989695992809665 28.944538578120376 17.68747568637971 16 18.835346061632976 33.809096905205735 27.43852714298052 19.917029890180775 17 16.519581205772138 33.921293037496284 29.650961500982483 19.908164255749096 18 18.10240266335887 33.43161959091211 30.038603723719316 18.4273740220097 19 17.61685632245841 36.6915745137467 29.491303653329236 16.200265510465652 20 16.92082759168862 34.10487281253845 29.79258236824024 19.181717227532687 21 17.5734452848964 35.115631565632825 29.70598957676531 17.604933572705463 22 15.475652751442004 34.186956358914514 30.81472887590634 19.522662013737147 23 15.489562626153774 32.886688784896016 30.298993521208356 21.324755067741854 24 14.445099176642415 33.31667205483242 30.384516322320838 21.853712446204323 25 16.275164835836726 33.78800280948898 29.443918365849576 20.492913988824714 26 17.487922483783912 33.16129416862895 29.410977948262907 19.939805399324225 27 16.392863775705564 33.17642688946923 28.63966775270688 21.79104158211832 28 18.36065248012302 33.3019214734073 28.908235333680317 19.429190712789364 29 18.666975435314114 35.49471386547011 26.839791107310095 18.998519591905676 30 17.180682393996438 35.180671694092815 27.29018062201597 20.348465289894783 31 18.545072961878535 34.48999291513524 26.194739774522457 20.77019434846376 32 18.206803151580182 33.93780146023113 27.66956863338538 20.1858267548033 33 17.83352937085331 31.994163966851698 29.385833174745475 20.786473487549515 34 16.975626383822355 31.7035087276821 30.277593713959476 21.043271174536063 35 17.735166685391498 31.551340812565964 30.168454696990192 20.545037805052342 36 17.193751561994862 33.23114925371994 31.035299746335852 18.53979943794935 37 18.457945175222388 32.93804832229333 29.669915615974347 18.934090886509942 38 17.93410846492304 33.817733255988315 30.43771012891091 17.81044815017773 39 16.78501524354127 32.893032299187645 30.423188831134883 19.898763626136194 40 17.017967431021923 32.654730159894775 31.065183048601252 19.262119360482046 41 15.497663981755135 31.116160446583407 33.37299940462679 20.013176167034665 42 17.499463094121705 31.446634612812524 31.624100157212155 19.42980213585362 43 16.625815963186216 30.983252357991258 32.43943281339445 19.95149886542808 44 18.284453880740404 30.841707918616535 30.468663421538757 20.40517477910431 45 18.69089736270304 30.712468368409912 31.421413411412058 19.175220857474994 46 16.61037753081381 31.881356411496892 31.49837628962499 20.009889768064305 47 17.357077948033623 30.842777908978974 30.41256535539348 21.38757878759392 48 17.815339534691763 30.389331278951836 32.29009272995048 19.505236456405918 49 18.447933122545233 29.83003203092578 30.176785336240652 21.54524951028834 50 17.238691157217506 29.771258988874393 30.34209884723824 22.64795100666986 51 17.364797164219826 29.10710068532883 31.93523806903425 21.592864081417098 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1029.0 1 895.0 2 761.0 3 666.5 4 572.0 5 792.5 6 1013.0 7 1030.0 8 1047.0 9 1199.5 10 1352.0 11 1598.5 12 1845.0 13 2159.0 14 2473.0 15 2826.5 16 3180.0 17 4052.0 18 4924.0 19 6402.0 20 7880.0 21 9965.0 22 12050.0 23 15562.5 24 19075.0 25 23766.5 26 33902.5 27 39347.0 28 44653.0 29 49959.0 30 55960.0 31 61961.0 32 65999.0 33 70037.0 34 72578.0 35 75119.0 36 77474.0 37 79829.0 38 80589.0 39 81349.0 40 79506.5 41 77664.0 42 77558.5 43 77453.0 44 82683.0 45 87913.0 46 93354.0 47 98795.0 48 104143.5 49 109492.0 50 102490.5 51 95489.0 52 81287.5 53 67086.0 54 56067.5 55 45049.0 56 37219.0 57 29389.0 58 24792.0 59 20195.0 60 18008.5 61 15822.0 62 13387.0 63 10952.0 64 9981.5 65 9011.0 66 7921.5 67 6832.0 68 5815.0 69 4798.0 70 4151.5 71 3505.0 72 2773.0 73 2041.0 74 1789.0 75 1202.5 76 868.0 77 653.5 78 439.0 79 391.0 80 343.0 81 317.0 82 291.0 83 194.5 84 98.0 85 73.5 86 49.0 87 35.5 88 22.0 89 14.5 90 7.0 91 10.0 92 13.0 93 9.0 94 5.0 95 4.0 96 3.0 97 2.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1308423.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.25116870535565 #Duplication Level Percentage of deduplicated Percentage of total 1 71.18839377581712 27.230392601820707 2 10.00952003721051 7.657516792059541 3 4.706843644259684 5.401268109189245 4 2.9015726648143767 4.439541820506523 5 1.9784048932881646 3.7838149670333365 6 1.469220532376572 3.371964146958522 7 1.1684258489377244 3.128555798719071 8 0.8928869890402594 2.7323176682076804 9 0.7695407744126579 2.6492250588937756 >10 4.759900431250863 32.52129426906578 >50 0.12624577647485868 3.033343708536645 >100 0.023966902425761414 1.7146551951594189 >500 0.0032497470025443904 0.8165391156693491 >1k 0.0018279826889312195 1.5195707481804754 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGCT 4607 0.3521032571270912 TruSeq Adapter, Index 13 (95% over 23bp) AATCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCT 3819 0.29187808529810316 No Hit AATGATACCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGT 2294 0.1753255636747443 No Hit AATGATACGGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCC 2203 0.16837062631885866 No Hit CAGCTGGGGAATGATACGGCTGTCTCTTATACACATCTGACGCCGAGACTT 1583 0.12098533883919804 No Hit CCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC 1555 0.11884535811431012 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.2928364909513207E-4 0.0 0.0 0.4075134723250814 0.0 2 2.2928364909513207E-4 0.0 0.0 0.7020665335292944 0.0 3 2.2928364909513207E-4 0.0 0.0 1.0488962667271975 0.0 4 2.2928364909513207E-4 0.0 0.0 2.258214659937956 0.0 5 2.2928364909513207E-4 0.0 0.0 2.6054265325510175 0.0 6 2.2928364909513207E-4 0.0 0.0 4.069020492608277 0.0 7 3.0571153212684277E-4 0.0 0.0 5.310591452458418 0.0 8 3.0571153212684277E-4 0.0 0.0 6.812475781914564 0.0 9 3.0571153212684277E-4 0.0 0.0 8.277904011164585 0.0 10 3.0571153212684277E-4 0.0 0.0 10.458238658293228 0.0 11 3.0571153212684277E-4 0.0 0.0 12.031277346851898 0.0 12 3.0571153212684277E-4 0.0 0.0 12.933967073339431 0.0 13 3.0571153212684277E-4 0.0 0.0 13.470185100689914 0.0 14 3.0571153212684277E-4 0.0 0.0 13.815562704110215 0.0 15 3.0571153212684277E-4 0.0 0.0 14.095976606953561 0.0 16 3.0571153212684277E-4 0.0 0.0 14.549499664863733 0.0 17 3.0571153212684277E-4 0.0 0.0 15.148312128417187 0.0 18 3.8213941515855346E-4 0.0 0.0 16.032582735094078 0.0 19 3.8213941515855346E-4 0.0 0.0 16.389195237320042 0.0 20 3.8213941515855346E-4 0.0 0.0 16.958582965906285 0.0 21 3.8213941515855346E-4 0.0 0.0 17.342709506023663 0.0 22 3.8213941515855346E-4 0.0 0.0 17.784921237245143 0.0 23 3.8213941515855346E-4 0.0 0.0 18.239514285517757 0.0 24 3.8213941515855346E-4 0.0 0.0 18.607361686549382 0.0 25 3.8213941515855346E-4 0.0 0.0 18.963897760892312 0.0 26 3.8213941515855346E-4 0.0 0.0 19.284283446561243 0.0 27 3.8213941515855346E-4 0.0 0.0 19.69791114952886 0.0 28 3.8213941515855346E-4 0.0 0.0 20.07179635331999 0.0 29 3.8213941515855346E-4 0.0 0.0 20.425428168107715 0.0 30 3.8213941515855346E-4 0.0 0.0 21.0399847755657 0.0 31 3.8213941515855346E-4 0.0 0.0 21.377184595501607 0.0 32 3.8213941515855346E-4 0.0 0.0 21.722409343155846 0.0 33 3.8213941515855346E-4 0.0 0.0 22.064347691839718 0.0 34 3.8213941515855346E-4 0.0 0.0 22.40972529526002 0.0 35 3.8213941515855346E-4 0.0 0.0 22.779941960665624 0.0 36 3.8213941515855346E-4 0.0 0.0 23.136019467710366 0.0 37 3.8213941515855346E-4 0.0 0.0 23.46855718678134 0.0 38 3.8213941515855346E-4 0.0 0.0 23.833423900374726 0.0 39 3.8213941515855346E-4 0.0 0.0 24.198749181266304 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGAGCG 40 3.4587174E-7 39.375 1 CAGCTGG 2050 0.0 39.07317 1 GGGAATG 4865 0.0 37.41521 7 TTACGGG 695 0.0 37.230217 3 CAGTACG 85 0.0 37.058826 1 TACGGGA 725 0.0 36.62069 4 TACGGGT 330 0.0 36.136364 4 GGAATGA 4550 0.0 35.901096 8 ATAGGGC 810 0.0 35.833332 4 ACTGGGC 775 0.0 35.70968 4 CTTTGCG 70 2.1827873E-11 35.357143 1 ACAGGGC 895 0.0 35.19553 4 CCTATGG 180 0.0 35.000004 2 TGCGGGC 605 0.0 34.95868 4 AGGGCTT 1405 0.0 34.911034 6 CTACTGG 355 0.0 34.859154 2 AACGGGC 795 0.0 34.81132 4 ACGGGAC 720 0.0 34.687504 5 ATAGGGA 850 0.0 34.676468 4 TTTGCGG 195 0.0 34.615383 2 >>END_MODULE