FastQCFastQC Report
Sat 18 Jun 2016
SRR3553153_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553153_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334377
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGCT52090.3903694383221533TruSeq Adapter, Index 13 (95% over 23bp)
AATCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCT43880.3288425984560585No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGT25230.18907699997826702No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCC24920.18675381844860936No Hit
CAGCTGGGGAATGATACGGCTGTCTCTTATACACATCTGACGCCGAGACTT18060.13534405943747532No Hit
CCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC16300.1221543836561931No Hit
AACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTG13650.10229492864460345No Hit
CAGCTGGGGAATGATACCTGTCTCTTATACACATCTGACGCCGAGACTTTC13490.10109586720994143No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGACC3800.036.7105266
CAGCTGG20350.036.7076151
GGGAATG47800.036.668417
TAGGGAC7450.036.5436255
GGAATGA42450.036.2014128
AGGGCAT15050.036.02996
GTACGCA250.002107230236.013
TATGGGC5500.035.5909084
CACGGGC9400.035.4255334
ACAGGGC9500.035.2894744
AAGGGCC7350.035.2040825
TAGGGCA7450.035.0335585
TTGTGCG458.7013177E-735.01
CATACGG3150.035.02
CAGGGCA10700.034.906545
CAAGGGC12700.034.9015734
AGGGCCC6900.034.8913046
TAACGGG7500.034.83
TAAGGGC6800.034.7426454
GCCAGCG653.6743586E-1034.6153871
Produced by FastQC (version 0.11.5)