FastQCFastQC Report
Sat 18 Jun 2016
SRR3553152_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553152_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997873
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGCT43930.44023638278618615No Hit
AATCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCT13430.13458626498562443No Hit
CCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC12450.12476537595465555No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12430.12456494964790109No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTAG451.9279469E-840.01
ATAGGGC5100.038.823534
ACGATAG356.247461E-638.5714261
TCGCTAG356.247461E-638.5714261
ACGTAGG1250.037.8000032
ACAGGGC9250.037.70274
AGAACGG2350.037.3404272
CTAGGGC6350.037.2047234
TAGGGCA6000.036.755
ACGGGCC5000.036.455
TCAGGGC5250.036.4285744
AGGGCCT8650.036.4161836
TACAGGG8900.036.4044953
AGGGCGC1550.036.290326
ACGCGTG250.00210697636.021
AACGGGC5500.035.5909084
AGGGCTC5950.035.5462236
ACAGGGA12100.035.516534
TTACGGG4950.035.4545443
TACCAGG2350.035.4255332
Produced by FastQC (version 0.11.5)