##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553152_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	997873
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.729035658846367	31.0	31.0	34.0	30.0	34.0
2	31.932678807824242	33.0	31.0	34.0	30.0	34.0
3	32.117078024959085	34.0	31.0	34.0	30.0	34.0
4	35.78112044318265	37.0	35.0	37.0	35.0	37.0
5	35.54596125959917	37.0	35.0	37.0	33.0	37.0
6	34.90138925494527	37.0	35.0	37.0	32.0	37.0
7	35.40140077945791	37.0	35.0	37.0	33.0	37.0
8	35.81255831152862	37.0	35.0	37.0	35.0	37.0
9	37.47427578459383	39.0	37.0	39.0	35.0	39.0
10	36.99933658892464	39.0	37.0	39.0	33.0	39.0
11	36.954097365095556	39.0	37.0	39.0	33.0	39.0
12	36.897899832944674	39.0	37.0	39.0	33.0	39.0
13	36.920441779665346	39.0	37.0	39.0	33.0	39.0
14	38.10872425649356	40.0	37.0	41.0	33.0	41.0
15	38.09062576099363	40.0	37.0	41.0	33.0	41.0
16	37.985680542513926	40.0	37.0	41.0	33.0	41.0
17	37.98761966703178	40.0	37.0	41.0	33.0	41.0
18	37.98222018232781	40.0	37.0	41.0	33.0	41.0
19	38.03228767588661	40.0	37.0	41.0	33.0	41.0
20	37.92879153960474	40.0	37.0	41.0	33.0	41.0
21	37.931949256067654	40.0	37.0	41.0	33.0	41.0
22	37.91653947947284	40.0	37.0	41.0	33.0	41.0
23	37.82212265488695	40.0	37.0	41.0	33.0	41.0
24	37.69964314096082	40.0	37.0	41.0	33.0	41.0
25	37.75074182786788	40.0	37.0	41.0	33.0	41.0
26	37.707415673136765	40.0	37.0	41.0	33.0	41.0
27	37.62778930785781	40.0	37.0	41.0	33.0	41.0
28	37.59672824096854	40.0	36.0	41.0	33.0	41.0
29	37.396161635799345	39.0	36.0	41.0	32.0	41.0
30	37.45637971966372	40.0	36.0	41.0	32.0	41.0
31	37.4364743810084	40.0	36.0	41.0	32.0	41.0
32	37.41013836430087	40.0	36.0	41.0	32.0	41.0
33	37.22402750650634	39.0	36.0	41.0	31.0	41.0
34	37.250095954094355	39.0	36.0	41.0	31.0	41.0
35	37.184228854774105	39.0	36.0	41.0	31.0	41.0
36	37.2010235771486	39.0	36.0	41.0	31.0	41.0
37	37.08364992338704	39.0	36.0	41.0	31.0	41.0
38	36.9311375295253	39.0	35.0	41.0	31.0	41.0
39	36.92447836548338	39.0	35.0	41.0	31.0	41.0
40	36.878547670896	39.0	35.0	41.0	31.0	41.0
41	36.82302256900427	39.0	35.0	41.0	31.0	41.0
42	36.740483007356644	39.0	35.0	41.0	31.0	41.0
43	36.65787229437012	39.0	35.0	40.0	31.0	41.0
44	36.54821405128709	39.0	35.0	40.0	31.0	41.0
45	36.360217181946	39.0	35.0	40.0	30.0	41.0
46	36.22538739899767	38.0	35.0	40.0	30.0	41.0
47	36.100176074510486	38.0	35.0	40.0	29.0	41.0
48	35.9842615242621	38.0	35.0	40.0	29.0	41.0
49	35.8704173777625	38.0	35.0	40.0	29.0	41.0
50	35.77139575877892	38.0	34.0	40.0	29.0	41.0
51	34.66436610670897	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	14.0
11	16.0
12	15.0
13	22.0
14	25.0
15	56.0
16	79.0
17	156.0
18	312.0
19	551.0
20	997.0
21	1478.0
22	2144.0
23	2877.0
24	3706.0
25	4834.0
26	5820.0
27	7474.0
28	9640.0
29	12871.0
30	16699.0
31	22122.0
32	28873.0
33	39357.0
34	58692.0
35	75004.0
36	93662.0
37	138124.0
38	213598.0
39	258568.0
40	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.814873235371635	30.49867067252045	21.23807338208369	27.448382710024223
2	20.850348691667175	28.217919514808	22.178974679142534	28.752757114382295
3	20.22181179368517	28.935545906142362	22.19260366800184	28.650038632170627
4	19.486347461049654	29.40524495602146	21.767499471375615	29.340908111553272
5	18.215444249919578	33.18448339618369	19.968472941947525	28.631599411949217
6	19.8004154837339	36.25050482376014	20.56884994383053	23.380229748675433
7	85.30384127038211	4.319186910558759	5.601213781713705	4.775758037345434
8	87.9273214126447	3.079850842742513	4.19271791099669	4.800109833616101
9	79.80725002079423	6.250394589291424	8.581452749999247	5.3609026399151
10	35.14615587354303	25.986473228557138	15.496861825101993	23.37050907279784
11	22.976470953718557	26.514496333701782	29.881758500330204	20.627274212249453
12	20.09033213645424	21.796160433241504	38.16888521886052	19.94462221144374
13	23.56692685341722	23.987020392374582	30.79530160651706	21.65075114769114
14	20.276327749122384	28.773902089744887	29.183272821290885	21.766497339841845
15	19.765841945818757	33.12786296452555	27.38194138933512	19.72435370032058
16	19.135701637382713	31.781599462055794	27.90425234473726	21.178446555824237
17	17.404920265404517	32.74915745791298	29.961027104651595	19.88489517203091
18	18.710697653909865	31.67717735623671	29.53361800549769	20.078506984355723
19	18.207627623956153	34.688783041529334	29.529509266209224	17.574080068305285
20	18.225665991564057	32.35752445451475	29.76410825826533	19.65270129565586
21	18.52470204124172	33.60928695334977	29.272562740950004	18.593448264458505
22	17.470559880866602	31.7740834755525	30.169270037369483	20.586086606211413
23	16.993445057637597	32.20099150893952	29.1053069879634	21.70025644545949
24	15.693880884641635	32.735929321667186	30.001914071229507	21.568275722461678
25	17.561352997826376	32.20339662462057	28.822705895439597	21.412544482113454
26	18.636640133564093	31.91057379045229	29.11422595861397	20.338560117369646
27	16.827492075644894	31.810059997614925	28.543512050130627	22.818935876609547
28	18.537829964334136	31.925405337152124	29.082859241606897	20.45390545690684
29	19.061744330190315	32.28887844445135	28.035130723047924	20.614246502310415
30	17.77931660642186	31.881812615433024	28.12031190341857	22.218558874726543
31	18.24180030925779	32.037944708394754	27.95004975583065	21.7702052265168
32	18.402141354661364	30.50498410118322	28.735921304614916	22.356953239540502
33	17.558346603225058	29.32798061476761	31.12029286291943	21.9933799190879
34	16.291051065616564	28.532789242719264	31.136226754306414	24.03993293735776
35	17.06970726735767	29.772025097382134	31.8066527504001	21.351614884860098
36	18.07885372186641	29.494835515140704	31.415320386462003	21.01099037653088
37	16.348874055115232	31.484567675445675	30.914254619575836	21.252303649863258
38	16.9912403682633	31.414518681234988	32.68822786065962	18.906013089842094
39	16.72597615127376	30.464097134605307	32.055281583929016	20.754645130191918
40	17.649440359644966	31.174909031510023	29.61328746243259	21.56236314641242
41	16.51442618449442	30.516308187514845	31.180621181252523	21.78864444673821
42	18.42849741399958	31.099648953323722	29.126351750172617	21.345501882504088
43	17.37836377975955	29.18066727930308	31.07579822281994	22.365170718117437
44	18.645859743674798	28.822806108592978	29.837163647077336	22.69417050065489
45	19.439848557882616	28.1474696679838	32.06089352051814	20.351788253615442
46	16.635483673774118	29.205820780800764	32.48439430669033	21.674301238734788
47	17.729009603426487	29.880856581949804	30.534647194582874	21.855486620040825
48	18.12384942773279	28.9459680740936	32.0725182463099	20.857664251863714
49	18.35594309095446	28.89265467649691	30.44395429077648	22.30744794177215
50	17.124323435948263	28.210403528304706	30.653299568181524	24.01197346756551
51	17.909994558425772	27.5585169655858	31.943844557373534	22.587643918614894
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1585.0
1	1283.5
2	982.0
3	734.5
4	487.0
5	632.5
6	778.0
7	838.5
8	899.0
9	1066.5
10	1234.0
11	1388.0
12	1542.0
13	1764.0
14	1986.0
15	2269.0
16	2552.0
17	3041.0
18	3530.0
19	4335.0
20	5140.0
21	6553.0
22	7966.0
23	10103.0
24	12240.0
25	15112.0
26	22014.0
27	26044.0
28	29446.0
29	32848.0
30	35864.5
31	38881.0
32	42819.5
33	46758.0
34	50100.0
35	53442.0
36	55703.0
37	57964.0
38	57732.5
39	57501.0
40	58031.0
41	58561.0
42	58005.0
43	57449.0
44	60121.0
45	62793.0
46	69333.5
47	75874.0
48	81173.0
49	86472.0
50	80284.0
51	74096.0
52	64319.0
53	54542.0
54	47127.0
55	39712.0
56	33140.5
57	26569.0
58	23306.5
59	20044.0
60	19444.0
61	18844.0
62	16334.5
63	13825.0
64	12551.0
65	11277.0
66	9181.5
67	7086.0
68	6568.0
69	6050.0
70	4951.0
71	3852.0
72	3579.0
73	3306.0
74	2683.5
75	1736.5
76	1412.0
77	1045.0
78	678.0
79	489.5
80	301.0
81	378.0
82	455.0
83	279.5
84	104.0
85	72.5
86	41.0
87	51.0
88	61.0
89	49.0
90	37.0
91	24.5
92	12.0
93	13.5
94	15.0
95	8.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	997873.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.989101907650785
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.41177491396974	24.952002987724395
2	9.846312622389611	6.693346462739775
3	4.020341380771581	4.0994337868377215
4	2.202485015304351	2.9944195054101357
5	1.5471347161886593	2.6292859766700363
6	1.1102506623556954	2.2641853743506712
7	0.8918458419438005	2.121912744541971
8	0.7254486270270343	1.9725877850229774
9	0.6231018421002914	1.9060804808992562
>10	5.100623796591978	35.047704521321606
>50	0.45113679674202006	10.368247004971375
>100	0.06624645000010335	3.6042819032191504
>500	0.002098303846059573	0.5086096579694523
>1k	0.0011990307691768988	0.8379018083214695
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGCT	4393	0.44023638278618615	No Hit
AATCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCT	1343	0.13458626498562443	No Hit
CCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC	1245	0.12476537595465555	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1243	0.12456494964790109	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4923472225423476	0.0
2	0.0	0.0	0.0	0.7931871089808021	0.0
3	1.0021315337723337E-4	0.0	0.0	1.140926751199802	0.0
4	1.0021315337723337E-4	0.0	0.0	2.4568256681962533	0.0
5	1.0021315337723337E-4	0.0	0.0	2.702748746583984	0.0
6	1.0021315337723337E-4	0.0	0.0	4.121366145792099	0.0
7	1.0021315337723337E-4	0.0	0.0	5.169395303811206	0.0
8	1.0021315337723337E-4	0.0	0.0	6.2668295464452894	0.0
9	1.0021315337723337E-4	0.0	0.0	7.490131509721177	0.0
10	1.0021315337723337E-4	0.0	0.0	9.402398902465544	0.0
11	1.0021315337723337E-4	0.0	0.0	10.957005550806565	0.0
12	1.0021315337723337E-4	0.0	0.0	11.696879262190679	0.0
13	2.0042630675446674E-4	0.0	0.0	12.016258582003923	0.0
14	2.0042630675446674E-4	0.0	0.0	12.290942835410918	0.0
15	2.0042630675446674E-4	0.0	0.0	12.525341401160269	0.0
16	2.0042630675446674E-4	0.0	0.0	13.035225925543632	0.0
17	2.0042630675446674E-4	0.0	0.0	13.780811786670247	0.0
18	2.0042630675446674E-4	0.0	0.0	14.615386927995846	0.0
19	2.0042630675446674E-4	0.0	0.0	15.05031201365304	0.0
20	3.006394601317001E-4	0.0	0.0	15.47271045513808	0.0
21	3.006394601317001E-4	0.0	0.0	15.92256730064848	0.0
22	3.006394601317001E-4	0.0	0.0	16.420125607166444	0.0
23	3.006394601317001E-4	0.0	0.0	16.93502078921867	0.0
24	3.006394601317001E-4	0.0	0.0	17.361026904225287	0.0
25	4.008526135089335E-4	0.0	0.0	17.736826229389912	0.0
26	4.008526135089335E-4	0.0	0.0	18.060214075338244	0.0
27	4.008526135089335E-4	0.0	0.0	18.433207432208306	0.0
28	4.008526135089335E-4	0.0	0.0	18.807002494305387	0.0
29	4.008526135089335E-4	0.0	0.0	19.197432939863088	0.0
30	4.008526135089335E-4	0.0	0.0	19.76494002743836	0.0
31	4.008526135089335E-4	0.0	0.0	20.11308052227087	0.0
32	4.008526135089335E-4	0.0	0.0	20.458916114575704	0.0
33	4.008526135089335E-4	0.0	0.0	20.806956396254833	0.0
34	4.008526135089335E-4	0.0	0.0	21.155096891087343	0.0
35	4.008526135089335E-4	0.0	0.0	21.51416061963797	0.0
36	4.008526135089335E-4	0.0	0.0	21.861399196090083	0.0
37	4.008526135089335E-4	0.0	0.0	22.201021572885526	0.0
38	5.010657668861668E-4	0.0	0.0	22.553270807006502	0.0
39	5.010657668861668E-4	0.0	0.0	22.91123219086998	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCACTAG	45	1.9279469E-8	40.0	1
ATAGGGC	510	0.0	38.82353	4
ACGATAG	35	6.247461E-6	38.571426	1
TCGCTAG	35	6.247461E-6	38.571426	1
ACGTAGG	125	0.0	37.800003	2
ACAGGGC	925	0.0	37.7027	4
AGAACGG	235	0.0	37.340427	2
CTAGGGC	635	0.0	37.204723	4
TAGGGCA	600	0.0	36.75	5
ACGGGCC	500	0.0	36.45	5
TCAGGGC	525	0.0	36.428574	4
AGGGCCT	865	0.0	36.416183	6
TACAGGG	890	0.0	36.404495	3
AGGGCGC	155	0.0	36.29032	6
ACGCGTG	25	0.002106976	36.0	21
AACGGGC	550	0.0	35.590908	4
AGGGCTC	595	0.0	35.546223	6
ACAGGGA	1210	0.0	35.51653	4
TTACGGG	495	0.0	35.454544	3
TACCAGG	235	0.0	35.425533	2
>>END_MODULE