FastQCFastQC Report
Sat 18 Jun 2016
SRR3553151_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553151_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012625
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGCT45180.4461671398592766No Hit
AATCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCT14910.14724108134798172No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13100.12936674484631527No Hit
CCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC12630.12472534255030243No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGT10490.10359214911739292No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCC10460.10329588939637083No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGCG800.039.3751
ATAGGGC4700.039.2553184
TAGGGCC4600.038.6413045
CTAGGGC6900.037.8260884
TGGGACT6250.037.4400026
TGGGTAC2050.037.3170746
AGGGCGT4000.037.1256
CCTGACG850.037.0588231
TAGCGAG552.748493E-936.818181
AATGGGC4800.036.56254
GTAGGGA4800.036.56254
GCAACGG3400.036.3970572
AGGGCCC5750.036.3913086
TGGGCAT7700.036.233776
CTGGGAC4550.036.09895
TTACGGG5300.036.0849083
CATGGGC7050.036.063834
ACAGGGC10300.036.043694
CCACGCG1500.036.01
CGATAGG1200.035.6252
Produced by FastQC (version 0.11.5)