##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553151_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1012625
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.755082088631035	31.0	31.0	34.0	30.0	34.0
2	31.949882730527097	33.0	31.0	34.0	30.0	34.0
3	32.11625330206147	34.0	31.0	34.0	30.0	34.0
4	35.780290828292806	37.0	35.0	37.0	35.0	37.0
5	35.55585927663252	37.0	35.0	37.0	33.0	37.0
6	35.573789902481174	37.0	35.0	37.0	33.0	37.0
7	35.759509196395506	37.0	35.0	37.0	35.0	37.0
8	35.816079002592275	37.0	35.0	37.0	35.0	37.0
9	37.50843895815331	39.0	37.0	39.0	35.0	39.0
10	37.02583483520553	39.0	37.0	39.0	33.0	39.0
11	36.97558424885817	39.0	37.0	39.0	33.0	39.0
12	36.95488359461795	39.0	37.0	39.0	33.0	39.0
13	36.970242686088135	39.0	37.0	39.0	33.0	39.0
14	38.14136921367732	40.0	37.0	41.0	33.0	41.0
15	38.12827354647575	40.0	37.0	41.0	33.0	41.0
16	38.03575558573016	40.0	37.0	41.0	33.0	41.0
17	38.00651475126528	40.0	37.0	41.0	33.0	41.0
18	37.96245698061968	40.0	37.0	41.0	33.0	41.0
19	38.03092260214788	40.0	37.0	41.0	33.0	41.0
20	37.94155289470436	40.0	37.0	41.0	33.0	41.0
21	37.924382668806324	40.0	37.0	41.0	33.0	41.0
22	37.89916207875571	40.0	37.0	41.0	33.0	41.0
23	37.77801752870016	40.0	37.0	41.0	33.0	41.0
24	37.66836983088508	40.0	37.0	41.0	32.0	41.0
25	37.71403629181582	40.0	37.0	41.0	33.0	41.0
26	37.66654388347118	40.0	37.0	41.0	33.0	41.0
27	37.61696185656092	40.0	37.0	41.0	33.0	41.0
28	37.55866979385261	40.0	36.0	41.0	32.0	41.0
29	37.4232751512159	40.0	36.0	41.0	32.0	41.0
30	37.461325762251576	40.0	36.0	41.0	32.0	41.0
31	37.4211292433033	39.0	36.0	41.0	32.0	41.0
32	37.38336797926182	39.0	36.0	41.0	32.0	41.0
33	37.35833502036785	39.0	36.0	41.0	32.0	41.0
34	37.32894210591285	39.0	36.0	41.0	32.0	41.0
35	37.23748870509814	39.0	36.0	41.0	31.0	41.0
36	37.20789137143562	39.0	36.0	41.0	31.0	41.0
37	37.083643007036166	39.0	36.0	41.0	31.0	41.0
38	37.039714603135415	39.0	35.0	41.0	31.0	41.0
39	36.98382323169979	39.0	35.0	41.0	31.0	41.0
40	36.90690927046044	39.0	35.0	41.0	31.0	41.0
41	36.790335020367856	39.0	35.0	41.0	31.0	41.0
42	36.69990766572028	39.0	35.0	40.0	31.0	41.0
43	36.61459869151957	39.0	35.0	40.0	31.0	41.0
44	36.52448339711147	39.0	35.0	40.0	30.0	41.0
45	36.354870756696705	39.0	35.0	40.0	30.0	41.0
46	36.22542500925812	38.0	35.0	40.0	30.0	41.0
47	36.070160227132455	38.0	35.0	40.0	29.0	41.0
48	35.981418590297494	38.0	35.0	40.0	29.0	41.0
49	35.8005500555487	38.0	34.0	40.0	29.0	41.0
50	35.70550993704481	38.0	34.0	40.0	28.0	41.0
51	34.52532773731638	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	7.0
11	12.0
12	9.0
13	13.0
14	34.0
15	40.0
16	92.0
17	193.0
18	328.0
19	565.0
20	911.0
21	1452.0
22	2085.0
23	2872.0
24	3735.0
25	4727.0
26	5921.0
27	7748.0
28	9872.0
29	13161.0
30	16888.0
31	22085.0
32	29301.0
33	39502.0
34	58795.0
35	74756.0
36	93634.0
37	138672.0
38	219051.0
39	266086.0
40	75.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.863103320577707	30.611529440809775	21.096950993704482	27.42841624490804
2	20.71675101839279	28.299123564991973	22.19212442908283	28.7920009875324
3	20.180520923342797	28.963337859523513	22.192519442044194	28.663621775089492
4	19.582767559560548	29.29346994198247	21.71692383656339	29.40683866189359
5	18.076188124922847	33.23569929638317	20.07199111220837	28.616121466485616
6	20.27295395630169	34.914109369213676	21.0118257005308	23.801110973953833
7	85.12509566720158	4.310578940871497	5.723639056906555	4.840686335020368
8	87.57397852117023	3.1582273793358846	4.326478212566349	4.94131588692754
9	79.64735217874338	6.24308110109863	8.693050240711022	5.416516479446982
10	35.072805826441176	25.93348969263054	15.534575978274287	23.459128502653993
11	22.78671768917418	26.45204295765955	30.116973213183556	20.64426613998272
12	20.00562893469942	21.64226638686582	38.4111591161585	19.940945562276262
13	23.493395877052215	23.97027527465745	30.783853845204295	21.75247500308604
14	20.200271571410937	28.707665720281444	29.40950499938279	21.682557708924826
15	19.725367238612517	32.9911862732996	27.527762004690782	19.75568448339711
16	19.04308110109863	31.829255647450932	27.953882236760897	21.173781014689546
17	17.27678064436489	32.81678805085792	30.036094309344524	19.870336995432663
18	18.66031354153808	31.53240340698679	29.624787063325513	20.18249598814961
19	18.006665843722995	34.742179977780516	29.650759165535117	17.60039501296136
20	18.124182199728427	32.42977410196272	29.839772867547214	19.606270830761634
21	18.266683125540055	33.74190840636959	29.245179607455867	18.74622886063449
22	17.315491914578445	31.866386865819035	30.259276632514503	20.558844587088014
23	17.0909270460437	32.135785705468464	29.057350944327858	21.71593630415998
24	15.599061844216763	32.73235403036662	29.92963831625725	21.738945809159365
25	17.589927169485247	32.25122824342674	28.75783236637452	21.401012220713493
26	18.616763362547832	32.046907789161835	29.125194420441918	20.211134427848414
27	16.833378595235157	31.677373163806937	28.54294531539316	22.946302925564748
28	18.475842488581655	31.778496481915813	29.17338600172818	20.57227502777435
29	19.14094556227626	32.15928897666955	28.005925194420445	20.69384026663375
30	17.72758918652018	31.872509566720158	28.107641031971365	22.2922602147883
31	18.152326873225526	31.985088260708554	27.961980002468835	21.90060486359709
32	18.368003950129612	30.471596099247005	28.714775953585974	22.445623997037405
33	17.43932847796568	29.208344648808787	31.20009875324034	22.15222811998519
34	16.248166892976176	28.50364152573756	31.00506110356746	24.2431304777188
35	17.042340451796075	29.60009875324034	31.854931489939514	21.502629305024072
36	18.04280952968769	29.369213677323785	31.51680039501296	21.07117639797556
37	16.300012344155043	31.3895074682138	30.876879397605233	21.433600790025924
38	16.834267374398223	31.339439575361066	32.83505740032095	18.991235649919762
39	16.669151956548575	30.39861745463523	32.24717936057277	20.685051228243427
40	17.622021972595974	31.133242809529687	29.690062955190715	21.55467226268362
41	16.410813479817307	30.532033082335513	31.273274904332798	21.783878533514383
42	18.396642389828415	31.02066411554129	29.144451302308354	21.438242192321937
43	17.375336378224908	29.193334156277	31.10835699296383	22.322972472534257
44	18.689248240957905	28.682088631033203	29.809949388964323	22.818713739044565
45	19.50109862979879	28.11396123935317	32.047204048882854	20.33773608196519
46	16.656807801505987	29.167460807307737	32.52665103073695	21.64908036044933
47	17.743685964695715	29.87433650166646	30.463399580298727	21.918577953339096
48	18.021873842735463	28.886310332057768	32.25705468460684	20.834761140599927
49	18.273497099123563	28.8429576595482	30.50596222688557	22.377583014442664
50	17.106036291815826	28.078311319590178	30.656363411924453	24.159288976669547
51	17.802839155659793	27.53743982224417	31.97323787186767	22.68648315022837
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1627.0
1	1316.5
2	1006.0
3	769.5
4	533.0
5	721.0
6	909.0
7	942.5
8	976.0
9	1133.0
10	1290.0
11	1402.5
12	1515.0
13	1770.0
14	2025.0
15	2309.5
16	2594.0
17	3094.5
18	3595.0
19	4398.5
20	5202.0
21	6663.5
22	8125.0
23	10176.5
24	12228.0
25	15141.0
26	22130.0
27	26206.0
28	29821.5
29	33437.0
30	36600.5
31	39764.0
32	43454.5
33	47145.0
34	50469.5
35	53794.0
36	56193.0
37	58592.0
38	58299.5
39	58007.0
40	58619.0
41	59231.0
42	58494.5
43	57758.0
44	60707.0
45	63656.0
46	70890.0
47	78124.0
48	83413.5
49	88703.0
50	82590.5
51	76478.0
52	66104.5
53	55731.0
54	47722.0
55	39713.0
56	33253.0
57	26793.0
58	23377.5
59	19962.0
60	19503.5
61	19045.0
62	16281.0
63	13517.0
64	12482.5
65	11448.0
66	9238.5
67	7029.0
68	6663.5
69	6298.0
70	5028.5
71	3759.0
72	3580.0
73	3401.0
74	2788.5
75	1765.5
76	1355.0
77	1056.5
78	758.0
79	552.0
80	346.0
81	397.5
82	449.0
83	271.5
84	94.0
85	72.5
86	51.0
87	62.0
88	73.0
89	50.0
90	27.0
91	22.0
92	17.0
93	12.5
94	8.0
95	4.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1012625.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.78820718534144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.6810995471157	24.89552257141713
2	9.640670367892424	6.514819355918624
3	3.8650124125968772	3.91775520512219
4	2.282331454716038	3.0846355223026887
5	1.5400396572607469	2.6017589506584167
6	1.1177683250327093	2.2660432650850355
7	0.9054664896125343	2.141586254528848
8	0.738167050803991	1.9953072999966168
9	0.604302160100449	1.8376457929221008
>10	5.080512515320611	34.778766768018656
>50	0.46993483362128424	10.66107703133855
>100	0.07116627950510371	3.8556760738482936
>500	0.0017644532107515029	0.38850874776002775
>1k	0.0017644532107515029	1.0608971610828175
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGCT	4518	0.4461671398592766	No Hit
AATCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCT	1491	0.14724108134798172	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1310	0.12936674484631527	No Hit
CCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC	1263	0.12472534255030243	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGT	1049	0.10359214911739292	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCC	1046	0.10329588939637083	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.875324034069869E-5	0.0	0.0	0.5078879150722133	0.0
2	9.875324034069869E-5	0.0	0.0	0.8221207258363165	0.0
3	9.875324034069869E-5	0.0	0.0	1.1979755585730156	0.0
4	9.875324034069869E-5	0.0	0.0	2.5961239353166277	0.0
5	9.875324034069869E-5	0.0	0.0	2.8549561782495987	0.0
6	9.875324034069869E-5	0.0	0.0	4.336748549561783	0.0
7	9.875324034069869E-5	0.0	0.0	5.435575854832737	0.0
8	9.875324034069869E-5	0.0	0.0	6.560375262313294	0.0
9	9.875324034069869E-5	0.0	0.0	7.824515491914578	0.0
10	9.875324034069869E-5	0.0	0.0	9.798395259844463	0.0
11	9.875324034069869E-5	0.0	0.0	11.424663621775089	0.0
12	9.875324034069869E-5	0.0	0.0	12.214195778298976	0.0
13	9.875324034069869E-5	0.0	0.0	12.547784224169856	0.0
14	9.875324034069869E-5	0.0	0.0	12.841575114183435	0.0
15	9.875324034069869E-5	0.0	0.0	13.093395877052215	0.0
16	9.875324034069869E-5	0.0	0.0	13.605530181459079	0.0
17	9.875324034069869E-5	0.0	0.0	14.35625231452907	0.0
18	9.875324034069869E-5	0.0	0.0	15.207011480064189	0.0
19	9.875324034069869E-5	0.0	0.0	15.633822984816689	0.0
20	9.875324034069869E-5	0.0	0.0	16.05638810023454	0.0
21	9.875324034069869E-5	0.0	0.0	16.53158869275398	0.0
22	9.875324034069869E-5	0.0	0.0	17.01913344031601	0.0
23	9.875324034069869E-5	0.0	0.0	17.536600419701273	0.0
24	9.875324034069869E-5	0.0	0.0	17.956202937908902	0.0
25	9.875324034069869E-5	0.0	0.0	18.338970497469447	0.0
26	9.875324034069869E-5	0.0	0.0	18.669102579928403	0.0
27	9.875324034069869E-5	0.0	0.0	19.05463523021849	0.0
28	9.875324034069869E-5	0.0	0.0	19.42209603752623	0.0
29	9.875324034069869E-5	0.0	0.0	19.806641155412912	0.0
30	9.875324034069869E-5	0.0	0.0	20.377731144303173	0.0
31	9.875324034069869E-5	0.0	0.0	20.73225527712628	0.0
32	9.875324034069869E-5	0.0	0.0	21.09833353906925	0.0
33	9.875324034069869E-5	0.0	0.0	21.44308110109863	0.0
34	9.875324034069869E-5	0.0	0.0	21.792568818664364	0.0
35	9.875324034069869E-5	0.0	0.0	22.147685470929513	0.0
36	9.875324034069869E-5	0.0	0.0	22.50043204542649	0.0
37	9.875324034069869E-5	0.0	0.0	22.840538205159856	0.0
38	9.875324034069869E-5	0.0	0.0	23.199308727317614	0.0
39	9.875324034069869E-5	0.0	0.0	23.562621898531045	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCGCG	80	0.0	39.375	1
ATAGGGC	470	0.0	39.255318	4
TAGGGCC	460	0.0	38.641304	5
CTAGGGC	690	0.0	37.826088	4
TGGGACT	625	0.0	37.440002	6
TGGGTAC	205	0.0	37.317074	6
AGGGCGT	400	0.0	37.125	6
CCTGACG	85	0.0	37.058823	1
TAGCGAG	55	2.748493E-9	36.81818	1
AATGGGC	480	0.0	36.5625	4
GTAGGGA	480	0.0	36.5625	4
GCAACGG	340	0.0	36.397057	2
AGGGCCC	575	0.0	36.391308	6
TGGGCAT	770	0.0	36.23377	6
CTGGGAC	455	0.0	36.0989	5
TTACGGG	530	0.0	36.084908	3
CATGGGC	705	0.0	36.06383	4
ACAGGGC	1030	0.0	36.04369	4
CCACGCG	150	0.0	36.0	1
CGATAGG	120	0.0	35.625	2
>>END_MODULE