##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553149_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1071103
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.800036971234327	33.0	31.0	34.0	30.0	34.0
2	32.014356229046136	33.0	31.0	34.0	30.0	34.0
3	32.154325027565044	34.0	31.0	34.0	30.0	34.0
4	35.81499911773191	37.0	35.0	37.0	35.0	37.0
5	35.59806666585753	37.0	35.0	37.0	33.0	37.0
6	35.60741403954615	37.0	35.0	37.0	33.0	37.0
7	35.77570130977133	37.0	35.0	37.0	35.0	37.0
8	35.84073520473755	37.0	35.0	37.0	35.0	37.0
9	37.47553316534451	39.0	37.0	39.0	35.0	39.0
10	37.076164477179134	39.0	37.0	39.0	33.0	39.0
11	37.028208304896914	39.0	37.0	39.0	33.0	39.0
12	37.01780220949806	39.0	37.0	39.0	33.0	39.0
13	37.03816439688807	39.0	37.0	39.0	33.0	39.0
14	38.215658064630574	40.0	38.0	41.0	33.0	41.0
15	38.20679057009457	40.0	37.0	41.0	33.0	41.0
16	38.0890735998312	40.0	37.0	41.0	33.0	41.0
17	38.05216678508042	40.0	37.0	41.0	33.0	41.0
18	37.967231909536245	40.0	37.0	41.0	33.0	41.0
19	38.05104084294414	40.0	37.0	41.0	33.0	41.0
20	37.983412426255924	40.0	37.0	41.0	33.0	41.0
21	37.98787885011992	40.0	37.0	41.0	33.0	41.0
22	37.96718709591888	40.0	37.0	41.0	33.0	41.0
23	37.84534260477284	40.0	37.0	41.0	33.0	41.0
24	37.77207607484994	40.0	37.0	41.0	33.0	41.0
25	37.76381449776539	40.0	37.0	41.0	33.0	41.0
26	37.7422115333446	40.0	37.0	41.0	33.0	41.0
27	37.678753583922365	40.0	37.0	41.0	33.0	41.0
28	37.573436915030584	40.0	37.0	41.0	33.0	41.0
29	37.41487513339053	40.0	36.0	41.0	32.0	41.0
30	37.45887930479142	40.0	36.0	41.0	32.0	41.0
31	37.445214885963345	40.0	36.0	41.0	32.0	41.0
32	37.3818811076059	40.0	36.0	41.0	32.0	41.0
33	37.30560739723444	39.0	36.0	41.0	32.0	41.0
34	37.30044076059912	39.0	36.0	41.0	32.0	41.0
35	37.21029816927037	39.0	36.0	41.0	31.0	41.0
36	37.09140297431713	39.0	36.0	41.0	31.0	41.0
37	37.03899438242634	39.0	35.0	41.0	31.0	41.0
38	36.95535256646653	39.0	35.0	41.0	31.0	41.0
39	36.895082919196376	39.0	35.0	41.0	31.0	41.0
40	36.84517828817583	39.0	35.0	41.0	31.0	41.0
41	36.80016954485236	39.0	35.0	41.0	31.0	41.0
42	36.7116028990676	39.0	35.0	40.0	31.0	41.0
43	36.61160131191865	39.0	35.0	40.0	31.0	41.0
44	36.57264800864156	39.0	35.0	40.0	31.0	41.0
45	36.46021717799315	39.0	35.0	40.0	30.0	41.0
46	36.335809908104075	38.0	35.0	40.0	30.0	41.0
47	36.22351445192479	38.0	35.0	40.0	30.0	41.0
48	36.15344182585615	38.0	35.0	40.0	30.0	41.0
49	35.95396801241337	38.0	35.0	40.0	29.0	41.0
50	35.83079405061885	38.0	35.0	40.0	29.0	41.0
51	34.639801214262306	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	3.0
10	6.0
11	8.0
12	3.0
13	16.0
14	20.0
15	37.0
16	94.0
17	147.0
18	277.0
19	582.0
20	1068.0
21	1684.0
22	2341.0
23	3131.0
24	3969.0
25	4874.0
26	5930.0
27	7646.0
28	9652.0
29	12900.0
30	17453.0
31	23082.0
32	31031.0
33	41977.0
34	62565.0
35	78835.0
36	97359.0
37	144803.0
38	232560.0
39	286970.0
40	77.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.756269938558663	32.260856332210814	21.884169869751087	26.098703859479432
2	20.484211135623745	29.032408647907808	22.24342570229007	28.239954514178372
3	19.83469376894659	27.711527276088294	23.335010731927742	29.11876822303737
4	19.887443131052756	27.648228041560895	21.437154036539905	31.027174790846445
5	17.83862056216816	32.56353497282707	20.319334368403414	29.27851009660135
6	20.101988324185445	32.75315259130075	21.746648081463686	25.398211003050125
7	84.64143971214719	4.6021717799315285	5.920252300665762	4.836136207255511
8	87.43351479736309	2.9482692140718494	4.200156287490558	5.4180597010745
9	79.55948214130667	6.0873697487543215	8.869081684954669	5.484066424984339
10	34.0760879205828	28.32043230202884	15.098454583732842	22.505025193655513
11	22.363208767037342	26.98134539815499	30.67230695834107	19.983138876466597
12	19.829092066776024	20.55918058300649	40.73305741838087	18.87866993183662
13	24.950821723027573	23.60557294676609	30.360665594251905	21.082939735954433
14	20.367975815584497	29.255636479404874	29.45561724689409	20.92077045811654
15	19.371526361143605	33.14116382831529	28.607332814864677	18.87997699567642
16	19.33866304174295	32.855196932507894	27.325756719941968	20.48038330580719
17	17.57375341120322	31.399407900080572	29.467940991669334	21.558897697046874
18	19.370125935600964	30.887505683393663	29.381301331431242	20.36106704957413
19	19.006948911542587	33.2605734462512	30.616663383446785	17.11581425875943
20	18.47562746066438	31.83466015873357	29.5949129075355	20.094799473066548
21	18.929552059885932	32.37671820543869	29.85296465419292	18.840765080482456
22	16.67075902130794	32.44655275916509	30.568208659671388	20.314479559855588
23	17.42325434622067	30.75278474619154	29.454870353271346	22.369090554316436
24	15.214036371852194	32.377651822467115	29.74979997255166	22.658511833129026
25	17.339228813662178	32.0990605011843	29.826169845477047	20.735540839676485
26	18.745069335068614	31.549066709737534	29.213063542908575	20.49280041228528
27	16.76141323476827	30.602845851426054	30.320520062029516	22.31522085177616
28	19.038318443697758	30.63505563890681	31.35375402739046	18.972871890004978
29	19.863822620233535	32.73522714435494	28.26973689738522	19.131213338026313
30	17.488140729696397	31.405009602251138	31.059571301732884	20.047278366319578
31	20.74020892481862	31.653818540327123	27.911041235063294	19.694931299790962
32	19.49224304291931	29.750733589580086	31.206335898601722	19.550687468898882
33	19.13149342313484	30.10933589019917	31.444128155742256	19.31504253092373
34	19.14465742323567	27.746257829545804	33.33909063834197	19.769994108876553
35	19.750201427873883	29.596406694780985	32.29913463037635	18.35425724696878
36	20.490653093119896	29.451135885157637	32.913921443595996	17.14428957812647
37	21.161550289748043	30.03352618749084	30.167873677881584	18.63704984487953
38	20.720229520410268	31.55093394379439	30.897121938786466	16.83171459700888
39	19.64432925685018	30.44366414807913	30.43190057352094	19.480106021549748
40	20.819099563720762	31.75726330707691	28.786587284322795	18.63704984487953
41	19.67691249114231	29.222866521707065	31.58939896536561	19.51082202178502
42	21.442008845087727	29.342556224751494	30.11503095407258	19.100403976088202
43	19.102457933550742	28.598183367986085	31.981238032196718	20.318120666266456
44	20.13401138826051	27.59445170072346	30.88274423654868	21.388792674467346
45	21.238106886079116	28.270483791007962	31.16376296210542	19.327646360807506
46	17.886235030617968	28.721980985955597	33.913825281042065	19.477958702384367
47	19.79800261972938	29.559155375346723	29.921772229188043	20.721069775735852
48	20.26229036796648	28.01812710822395	32.62263293072655	19.096949593083018
49	21.04512824630311	27.758394850915362	30.71889444805962	20.477582454721908
50	18.955599974979066	27.441711954872687	30.20876610372672	23.39392196642153
51	19.010029847736398	26.920660291307186	33.0036420400279	21.065667820928518
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1417.0
1	1227.0
2	1037.0
3	778.5
4	520.0
5	679.0
6	838.0
7	873.5
8	909.0
9	980.0
10	1051.0
11	1268.0
12	1485.0
13	1688.0
14	1891.0
15	2034.0
16	2177.0
17	2812.5
18	3448.0
19	4232.5
20	5017.0
21	6757.5
22	8498.0
23	10975.5
24	13453.0
25	16473.0
26	22841.5
27	26190.0
28	30601.5
29	35013.0
30	39802.5
31	44592.0
32	46618.5
33	48645.0
34	51591.5
35	54538.0
36	56804.0
37	59070.0
38	60130.0
39	61190.0
40	62364.5
41	63539.0
42	63019.0
43	62499.0
44	69659.5
45	76820.0
46	80834.5
47	84849.0
48	90063.0
49	95277.0
50	88462.0
51	81647.0
52	69933.0
53	58219.0
54	49924.5
55	41630.0
56	34946.0
57	28262.0
58	24691.5
59	21121.0
60	19469.5
61	17818.0
62	16166.5
63	14515.0
64	12558.5
65	10602.0
66	9031.0
67	7460.0
68	6130.0
69	4800.0
70	4140.5
71	3481.0
72	3337.0
73	3193.0
74	2593.0
75	1616.5
76	1240.0
77	917.0
78	594.0
79	580.5
80	567.0
81	402.0
82	237.0
83	181.5
84	126.0
85	98.5
86	71.0
87	48.0
88	25.0
89	26.5
90	28.0
91	21.0
92	14.0
93	8.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1071103.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.392576206854066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.57082263952148	23.831484789516438
2	9.783788012634412	6.338441975819309
3	3.9447829633590246	3.8334504828032046
4	2.2049161994447872	2.8569166408096924
5	1.4870285224488717	2.4084342367595335
6	1.0807413653986697	2.100479822314555
7	0.8786052777274967	1.9922201890172488
8	0.7177678510255074	1.86002798505389
9	0.5894837611419851	1.718540788994524
>10	5.120798529634992	33.55509569840801
>50	0.5194614646249741	11.446273281425047
>100	0.09484249590802632	4.717366100478911
>500	0.0034804585648948614	0.7368565119628564
>1k	0.003190420351153623	1.974377100458935
>5k	2.9003821374123847E-4	0.6300343961778547
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT	6706	0.6260835792636189	Illumina Single End Adapter 2 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCT	3620	0.3379693642908292	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCC	3322	0.31014757684368355	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGT	3234	0.30193174699351977	No Hit
ACCCCCGGGAATGATACCTGTCTCTTATACACATCTGACGCTGTGTAGGTC	2542	0.2373254486263226	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	1521	0.1420031500238539	Illumina Single End Adapter 2 (95% over 21bp)
ACCCCCGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGTAGG	1451	0.13546783082485997	No Hit
ACCCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCT	1149	0.1072725965663433	No Hit
ACCCCCGGGAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCT	1114	0.10400493696684633	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.336170284277049E-5	0.0	0.0	0.6918102180649294	0.0
2	9.336170284277049E-5	0.0	0.0	1.0176425609861983	0.0
3	9.336170284277049E-5	0.0	0.0	1.4523346494221376	0.0
4	9.336170284277049E-5	0.0	0.0	3.133405470809063	0.0
5	9.336170284277049E-5	0.0	0.0	3.437297813562281	0.0
6	9.336170284277049E-5	0.0	0.0	4.911665824855313	0.0
7	9.336170284277049E-5	0.0	0.0	5.987659450118243	0.0
8	9.336170284277049E-5	0.0	0.0	6.94695094682771	0.0
9	9.336170284277049E-5	0.0	0.0	8.263631042019302	0.0
10	9.336170284277049E-5	0.0	0.0	9.94647573576024	0.0
11	9.336170284277049E-5	0.0	0.0	11.606913620818913	0.0
12	9.336170284277049E-5	0.0	0.0	12.377334392677454	0.0
13	9.336170284277049E-5	0.0	0.0	12.758810310493015	0.0
14	9.336170284277049E-5	0.0	0.0	13.099580525869127	0.0
15	9.336170284277049E-5	0.0	0.0	13.37229005987286	0.0
16	9.336170284277049E-5	0.0	0.0	13.930593042872628	0.0
17	9.336170284277049E-5	0.0	0.0	14.743026581010417	0.0
18	9.336170284277049E-5	0.0	0.0	16.0404741654164	0.0
19	9.336170284277049E-5	0.0	0.0	16.493651871015206	0.0
20	9.336170284277049E-5	0.0	0.0	17.162495110180814	0.0
21	9.336170284277049E-5	0.0	0.0	17.646108730906366	0.0
22	1.8672340568554098E-4	0.0	0.0	18.23540779924993	0.0
23	1.8672340568554098E-4	0.0	0.0	18.817798101583133	0.0
24	1.8672340568554098E-4	0.0	0.0	19.25305036023613	0.0
25	1.8672340568554098E-4	0.0	0.0	19.644142533444494	0.0
26	1.8672340568554098E-4	0.0	0.0	19.973989429588006	0.0
27	1.8672340568554098E-4	0.0	0.0	20.5338795615361	0.0
28	1.8672340568554098E-4	0.0	0.0	20.951859905163182	0.0
29	1.8672340568554098E-4	0.0	0.0	21.336416759172554	0.0
30	1.8672340568554098E-4	0.0	0.0	22.06687872221439	0.0
31	1.8672340568554098E-4	0.0	0.0	22.43276323565521	0.0
32	1.8672340568554098E-4	0.0	0.0	22.789218217108907	0.0
33	1.8672340568554098E-4	0.0	0.0	23.135029964438527	0.0
34	1.8672340568554098E-4	0.0	0.0	23.489711073538214	0.0
35	1.8672340568554098E-4	0.0	0.0	23.842898395392414	0.0
36	1.8672340568554098E-4	0.0	0.0	24.18385533417421	0.0
37	1.8672340568554098E-4	0.0	0.0	24.51332878350635	0.0
38	1.8672340568554098E-4	0.0	0.0	24.855686147830788	0.0
39	1.8672340568554098E-4	0.0	0.0	25.206446065411075	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCG	40	6.8139343E-9	45.000004	1
CCCCCGG	1945	0.0	41.876606	2
ACCCCCG	1845	0.0	40.85366	1
CCCGGGA	2230	0.0	40.661434	4
CGGGAAT	2720	0.0	40.202206	6
CCCCGGG	2360	0.0	39.947033	3
CTACTAG	85	0.0	39.705883	1
CCGATAG	40	3.4579352E-7	39.375004	1
ACGCTAG	40	3.4579352E-7	39.375004	1
TACTACG	40	3.4579352E-7	39.375004	1
CCGGGAA	2310	0.0	39.05844	5
GGGAATG	4480	0.0	38.219864	7
ACGGGTA	395	0.0	37.594936	5
GCCTCGG	270	0.0	37.5	2
GGAATGA	4225	0.0	37.22485	8
TGCTACG	115	0.0	37.173912	1
CTTACGG	250	0.0	36.899998	2
CAGACGG	185	0.0	36.486485	2
AGGGCCT	870	0.0	36.465515	6
CTCGCGG	180	0.0	36.25	2
>>END_MODULE