FastQCFastQC Report
Sat 18 Jun 2016
SRR3553148_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553148_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324506
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGCT56180.4241581389589779No Hit
AATCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCT48820.3685902517617889No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGT39760.30018739061959704No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCC39210.2960348990491549No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16270.1228382506383512No Hit
CCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC15140.11430676795726104No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGG3000.041.252
CGTACAG750.039.01
CGCACCG750.039.01
GCTAGCG1100.038.863641
CCACACG1550.037.7419361
CTAGCGG3900.036.923082
CGGGCTA2500.036.8999986
ACGGGTC2200.036.8181845
CCACGCG552.750312E-936.8181841
CGAGGGC5250.036.4285744
AGGGCGT4950.036.3636366
ACGGGAC7750.036.290325
GTAGGGA8450.036.213024
ACGGGCT10600.036.0849085
CGGGTAT5800.036.0775836
AGTAGGG9750.036.03
CGCGTAG751.8189894E-1236.01
GGCACCG1900.035.5263148
CCTAGGG8850.035.3389853
ACGGGCG3450.035.2173925
Produced by FastQC (version 0.11.5)