##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553148_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1324506
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.763896879289334	31.0	31.0	34.0	30.0	34.0
2	31.959177232870218	33.0	31.0	34.0	30.0	34.0
3	32.14058750960736	34.0	31.0	34.0	30.0	34.0
4	35.78798963538104	37.0	35.0	37.0	35.0	37.0
5	35.55828059669039	37.0	35.0	37.0	33.0	37.0
6	34.857255459771416	37.0	35.0	37.0	32.0	37.0
7	35.382684563150335	37.0	35.0	37.0	33.0	37.0
8	35.82388452751441	37.0	35.0	37.0	35.0	37.0
9	37.504607755646255	39.0	37.0	39.0	35.0	39.0
10	37.018190933072404	39.0	37.0	39.0	33.0	39.0
11	36.98589738362831	39.0	37.0	39.0	33.0	39.0
12	36.93729511229092	39.0	37.0	39.0	33.0	39.0
13	36.96226819659556	39.0	37.0	39.0	33.0	39.0
14	38.152511200402266	40.0	37.0	41.0	33.0	41.0
15	38.13801371983215	40.0	37.0	41.0	33.0	41.0
16	38.01690366068557	40.0	37.0	41.0	33.0	41.0
17	38.0257001478287	40.0	37.0	41.0	33.0	41.0
18	37.99284638952183	40.0	37.0	41.0	33.0	41.0
19	38.050386332715746	40.0	37.0	41.0	33.0	41.0
20	37.978687903263555	40.0	37.0	41.0	33.0	41.0
21	38.01634647181666	40.0	37.0	41.0	33.0	41.0
22	37.98546250451112	40.0	37.0	41.0	33.0	41.0
23	37.89841042622683	40.0	37.0	41.0	33.0	41.0
24	37.77312447055733	40.0	37.0	41.0	33.0	41.0
25	37.842101885533175	40.0	37.0	41.0	33.0	41.0
26	37.79669099271729	40.0	37.0	41.0	33.0	41.0
27	37.709524154665964	40.0	37.0	41.0	33.0	41.0
28	37.71081822203901	40.0	37.0	41.0	33.0	41.0
29	37.506345007119634	40.0	36.0	41.0	32.0	41.0
30	37.54275254321234	40.0	36.0	41.0	32.0	41.0
31	37.53841507701739	40.0	36.0	41.0	32.0	41.0
32	37.47881700800147	40.0	36.0	41.0	32.0	41.0
33	37.30723228131847	40.0	36.0	41.0	32.0	41.0
34	37.29616173879167	39.0	36.0	41.0	32.0	41.0
35	37.287632521105984	39.0	36.0	41.0	32.0	41.0
36	37.22790232735827	39.0	36.0	41.0	32.0	41.0
37	37.18361638225874	39.0	36.0	41.0	31.0	41.0
38	37.02660539099106	39.0	36.0	41.0	31.0	41.0
39	36.96407641792487	39.0	35.0	41.0	31.0	41.0
40	36.912053248531905	39.0	35.0	41.0	31.0	41.0
41	36.875858622006994	39.0	35.0	41.0	31.0	41.0
42	36.80409601768508	39.0	35.0	41.0	31.0	41.0
43	36.73493060809086	39.0	35.0	40.0	31.0	41.0
44	36.66088639840061	39.0	35.0	40.0	31.0	41.0
45	36.48281245989071	39.0	35.0	40.0	30.0	41.0
46	36.33188147128061	39.0	35.0	40.0	30.0	41.0
47	36.23691700905847	38.0	35.0	40.0	30.0	41.0
48	36.1276173909367	38.0	35.0	40.0	30.0	41.0
49	36.03038340332169	38.0	35.0	40.0	29.0	41.0
50	35.929357058405174	38.0	35.0	40.0	29.0	41.0
51	34.797261016560135	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	7.0
11	13.0
12	9.0
13	10.0
14	32.0
15	54.0
16	128.0
17	185.0
18	379.0
19	682.0
20	1151.0
21	1800.0
22	2554.0
23	3621.0
24	4556.0
25	5703.0
26	7341.0
27	9505.0
28	12507.0
29	16382.0
30	21790.0
31	29039.0
32	38378.0
33	52203.0
34	76860.0
35	97642.0
36	122267.0
37	180760.0
38	282742.0
39	356087.0
40	113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.14426510714183	32.11793680058829	21.04830027195045	26.689497820319424
2	20.985861898700346	29.587861436641283	22.16803849888185	27.258238165776522
3	20.872536628750645	29.09424343868582	22.53851624681202	27.49470368575152
4	19.866501171002625	29.317722985022343	21.14501557561838	29.670760268356656
5	18.031628395794357	33.10653179374046	20.403456080984157	28.458383729481028
6	19.6060266997658	35.74072144633546	21.36381413145731	23.289437722441424
7	85.18458957528317	4.669967519965935	5.505826323172564	4.6396165815783394
8	87.70945544980545	2.99447492121591	3.9979433841749303	5.298126244803723
9	79.5295000551149	6.539645724519179	8.346961055669057	5.5838931646968755
10	33.713701561185836	27.88194239965693	15.402421733083882	23.00193430607336
11	22.347275135031474	25.612870005873887	31.39389327039666	20.645961588697975
12	21.029651809806825	20.86808213779326	38.66732200533633	19.434944047063585
13	24.01038575891691	23.943039895629013	30.68276021399677	21.36381413145731
14	19.9686524636355	28.01353863251658	30.475286635168132	21.542522268679797
15	19.541172331420167	33.26840346514096	28.323088004131353	18.867336199307516
16	19.981260938040297	30.936364199180677	28.459742726722265	20.622632136056765
17	18.31414882227789	31.65836923351046	30.194502705159508	19.832979239052147
18	17.706224056365166	31.859500825213328	29.93138573928695	20.502889379134558
19	17.801882362178805	34.92426610373981	30.200618192745072	17.073233341336316
20	18.528568387006175	32.81276189009336	29.936670728558422	18.721998994342044
21	18.55725832876559	32.70328711232716	29.496657621785026	19.24279693712222
22	16.75681348366863	32.082300872929224	30.792159491916234	20.368726151485912
23	17.26545595112442	31.668259713432782	28.920593791194605	22.145690544248193
24	15.737263553354985	32.94058313061625	29.80031800535445	21.52183531067432
25	17.621890727561823	31.205068153711647	29.17366927745137	21.999371841275163
26	18.41675311399118	32.526239971732856	29.194960234230727	19.86204668004524
27	16.059949898301706	31.84039936398929	30.03512252870127	22.064528209007737
28	18.187309079762567	30.82039643459524	30.230516132052255	20.76177835358994
29	18.395084657978146	32.93076815054066	29.08827895079373	19.58586824068747
30	18.458655528929278	32.65904420214027	27.827129510927094	21.05517075800336
31	17.155603674124542	32.267048997890534	29.281936057669807	21.29541127031512
32	18.806860822072533	32.521558981235266	28.39300086220825	20.27857933448395
33	19.090211746870153	29.773289060223206	30.48381811784922	20.652681075057416
34	15.813442898710917	30.404845278164082	31.19736716934465	22.584344653780352
35	18.600142241711247	30.281025529518175	31.01307204346375	20.105760185306824
36	17.484028007423145	31.7542540388643	31.524281505708547	19.23743644800401
37	18.406787134222117	32.50540201403391	29.22870866572141	19.85910218602256
38	19.730299447492122	31.702763143390815	29.45958719703799	19.10735021207907
39	18.949102533321856	31.272640516539752	28.979483671648147	20.798773278490245
40	21.014325340919555	30.08706642325516	28.240642171496393	20.65796606432889
41	18.702671033577804	29.365740887546	30.388763810809465	21.542824268066735
42	19.585490741453796	30.763243050616605	28.745736146155625	20.905530061773973
43	17.69263408395281	29.383785350915737	31.444251668169116	21.47932889696234
44	19.232830957353155	28.85475792484141	29.7042067004604	22.20820441734503
45	20.359137670950528	29.280728060122037	30.425758735709767	19.93437553321767
46	17.376516225672063	30.50888406696534	31.562484428156612	20.55211527920598
47	19.0131264033534	29.89537231239421	29.167931289099485	21.92356999515291
48	18.68825056285136	29.00983461003574	31.708425631895963	20.593489195216932
49	18.93777755631156	28.877030379628327	30.39465279885482	21.790539265205293
50	17.700410568166546	28.702625733669763	29.515381583775387	24.081582114388308
51	18.082062293413546	27.7841700981347	32.18762315912499	21.94614444932677
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2133.0
1	1679.0
2	1225.0
3	910.0
4	595.0
5	719.5
6	844.0
7	957.5
8	1071.0
9	1173.5
10	1276.0
11	1591.5
12	1907.0
13	2076.0
14	2245.0
15	2557.0
16	2869.0
17	3468.5
18	4068.0
19	5472.0
20	6876.0
21	8591.0
22	10306.0
23	12502.5
24	14699.0
25	18742.5
26	27586.5
27	32387.0
28	37697.0
29	43007.0
30	48359.0
31	53711.0
32	57722.0
33	61733.0
34	65647.5
35	69562.0
36	73275.5
37	76989.0
38	78467.0
39	79945.0
40	78749.5
41	77554.0
42	79070.5
43	80587.0
44	89831.5
45	99076.0
46	102391.0
47	105706.0
48	108183.5
49	110661.0
50	102984.0
51	95307.0
52	82769.5
53	70232.0
54	59591.5
55	48951.0
56	42536.5
57	36122.0
58	32226.0
59	28330.0
60	25878.5
61	23427.0
62	20861.0
63	18295.0
64	15383.5
65	12472.0
66	11133.5
67	9795.0
68	7892.0
69	5989.0
70	4720.5
71	3452.0
72	2927.0
73	2402.0
74	2262.5
75	1988.5
76	1854.0
77	1408.0
78	962.0
79	695.5
80	429.0
81	364.5
82	300.0
83	220.0
84	140.0
85	97.0
86	54.0
87	39.0
88	24.0
89	18.0
90	12.0
91	12.0
92	12.0
93	8.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1324506.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.31912183526471
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.497107423414	21.548706470866133
2	9.990425417009007	5.8582099997482455
3	3.934015481270526	3.460256375915643
4	2.1929009004580986	2.571757146927707
5	1.3754505976405722	2.0163501825305796
6	0.9971050523864985	1.7540546708086648
7	0.7928504787616012	1.6271975848771536
8	0.6547475824415467	1.5357299312758987
9	0.5474511116910714	1.444570725826947
>10	5.0457960428821345	31.42736054563182
>50	0.7408905236692501	15.14325116660557
>100	0.22573442006828265	9.089102874934321
>500	0.0031571247471357654	0.6126973714599412
>1k	0.0021047498314238433	1.4774005735329538
>5k	2.630937289279804E-4	0.43335437905853574
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGCT	5618	0.4241581389589779	No Hit
AATCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCT	4882	0.3685902517617889	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGT	3976	0.30018739061959704	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCC	3921	0.2960348990491549	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1627	0.1228382506383512	No Hit
CCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	1514	0.11430676795726104	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5099969347062226E-4	0.0	0.0	0.4788200279953432	0.0
2	1.5099969347062226E-4	0.0	0.0	0.759301958617024	0.0
3	1.5099969347062226E-4	0.0	0.0	1.1367256924468443	0.0
4	1.5099969347062226E-4	0.0	0.0	2.6184101846273253	0.0
5	1.5099969347062226E-4	0.0	0.0	2.867333179313646	0.0
6	1.5099969347062226E-4	0.0	0.0	4.233502905989101	0.0
7	1.5099969347062226E-4	0.0	0.0	5.30477023131643	0.0
8	1.5099969347062226E-4	0.0	0.0	6.311107688451393	0.0
9	1.5099969347062226E-4	0.0	0.0	7.707930352901384	0.0
10	1.5099969347062226E-4	0.0	0.0	9.531025152018941	0.0
11	1.5099969347062226E-4	0.0	0.0	11.25008116233524	0.0
12	1.5099969347062226E-4	0.0	0.0	11.970425200036845	0.0
13	1.5099969347062226E-4	0.0	0.0	12.281333568892855	0.0
14	1.5099969347062226E-4	0.0	0.0	12.586277449856777	0.0
15	1.5099969347062226E-4	0.0	0.0	12.818665978108065	0.0
16	1.5099969347062226E-4	0.0	0.0	13.29310701499276	0.0
17	1.5099969347062226E-4	0.0	0.0	13.992462095301947	0.0
18	1.5099969347062226E-4	0.0	0.0	14.79057097514092	0.0
19	1.5099969347062226E-4	0.0	0.0	15.198723146592013	0.0
20	1.5099969347062226E-4	0.0	0.0	15.588981854366835	0.0
21	1.5099969347062226E-4	0.0	0.0	16.017971983516873	0.0
22	1.5099969347062226E-4	0.0	0.0	16.497169510745895	0.0
23	1.5099969347062226E-4	0.0	0.0	16.983086524334354	0.0
24	1.5099969347062226E-4	0.0	0.0	17.378554721533916	0.0
25	1.5099969347062226E-4	0.0	0.0	17.721550525252432	0.0
26	2.2649954020593339E-4	0.0	0.0	18.04061287755586	0.0
27	2.2649954020593339E-4	0.0	0.0	18.399312649395323	0.0
28	2.2649954020593339E-4	0.0	0.0	18.735664466601133	0.0
29	2.2649954020593339E-4	0.0	0.0	19.09866772970451	0.0
30	2.2649954020593339E-4	0.0	0.0	19.661292587576046	0.0
31	2.2649954020593339E-4	0.0	0.0	20.00036239926433	0.0
32	2.2649954020593339E-4	0.0	0.0	20.34139520696773	0.0
33	2.2649954020593339E-4	0.0	0.0	20.6877130039426	0.0
34	3.7749923367655564E-4	0.0	0.0	21.016892335708558	0.0
35	3.7749923367655564E-4	0.0	0.0	21.35875564172605	0.0
36	3.7749923367655564E-4	0.0	0.0	21.701977944984773	0.0
37	3.7749923367655564E-4	0.0	0.0	22.046634745331467	0.0
38	3.7749923367655564E-4	0.0	0.0	22.393934040313898	0.0
39	3.7749923367655564E-4	0.0	0.0	22.76471378763101	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGCGG	300	0.0	41.25	2
CGTACAG	75	0.0	39.0	1
CGCACCG	75	0.0	39.0	1
GCTAGCG	110	0.0	38.86364	1
CCACACG	155	0.0	37.741936	1
CTAGCGG	390	0.0	36.92308	2
CGGGCTA	250	0.0	36.899998	6
ACGGGTC	220	0.0	36.818184	5
CCACGCG	55	2.750312E-9	36.818184	1
CGAGGGC	525	0.0	36.428574	4
AGGGCGT	495	0.0	36.363636	6
ACGGGAC	775	0.0	36.29032	5
GTAGGGA	845	0.0	36.21302	4
ACGGGCT	1060	0.0	36.084908	5
CGGGTAT	580	0.0	36.077583	6
AGTAGGG	975	0.0	36.0	3
CGCGTAG	75	1.8189894E-12	36.0	1
GGCACCG	190	0.0	35.526314	8
CCTAGGG	885	0.0	35.338985	3
ACGGGCG	345	0.0	35.217392	5
>>END_MODULE