FastQCFastQC Report
Sat 18 Jun 2016
SRR3553147_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553147_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1351106
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGCT60690.4491875544923936No Hit
AATCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCT54820.4057416664569619No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCC45920.33986970674395645No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGT44760.3312841479499018No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16500.1221221725016394No Hit
CCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC15950.11805143341825143No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTC2400.040.3124965
ACGGGCC5800.039.9568945
CTATGCG700.038.5714261
TCGCGCG1000.038.251
CGCTGCG950.037.8947371
AGGGCGT4850.037.1134036
TAGGGAG7350.037.0408175
CATGGGA8750.037.028574
TGCGGGC5300.036.5094344
GTACGGG4750.036.4736823
TAGGGAC6850.036.4598545
TACTAGG3400.036.397062
ACTATGG3300.036.1363642
CGAGGGC4300.036.1046524
ACAGGGC8850.036.1016964
CCTAGGG9000.036.03
ACGTGTC250.002107239636.013
AACGGGC7450.035.6375854
CAGGGAT14700.035.5102045
TATGGGC6600.035.4545444
Produced by FastQC (version 0.11.5)