##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553147_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1351106 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.770797405977028 31.0 31.0 34.0 30.0 34.0 2 31.96761023931505 33.0 31.0 34.0 30.0 34.0 3 32.12793296750958 34.0 31.0 34.0 30.0 34.0 4 35.7839903012791 37.0 35.0 37.0 35.0 37.0 5 35.56248140412373 37.0 35.0 37.0 33.0 37.0 6 35.58534489521917 37.0 35.0 37.0 33.0 37.0 7 35.76390231410415 37.0 35.0 37.0 35.0 37.0 8 35.835051431937984 37.0 35.0 37.0 35.0 37.0 9 37.539270049870254 39.0 37.0 39.0 35.0 39.0 10 37.038333039746696 39.0 37.0 39.0 33.0 39.0 11 37.003545243674445 39.0 37.0 39.0 33.0 39.0 12 36.986504389737 39.0 37.0 39.0 33.0 39.0 13 37.00159720998945 39.0 37.0 39.0 33.0 39.0 14 38.18479083062321 40.0 38.0 41.0 33.0 41.0 15 38.169609194245304 40.0 37.0 41.0 33.0 41.0 16 38.06234151872614 40.0 37.0 41.0 33.0 41.0 17 38.03822942093367 40.0 37.0 41.0 33.0 41.0 18 37.969676694500656 40.0 37.0 41.0 33.0 41.0 19 38.0532667311077 40.0 37.0 41.0 33.0 41.0 20 37.993762887589874 40.0 37.0 41.0 33.0 41.0 21 38.0125356559737 40.0 37.0 41.0 33.0 41.0 22 37.972495126215115 40.0 37.0 41.0 33.0 41.0 23 37.85622445611225 40.0 37.0 41.0 33.0 41.0 24 37.744528556604735 40.0 37.0 41.0 33.0 41.0 25 37.818013538538054 40.0 37.0 41.0 33.0 41.0 26 37.75596215248841 40.0 37.0 41.0 33.0 41.0 27 37.70848105181977 40.0 37.0 41.0 33.0 41.0 28 37.68805926403998 40.0 37.0 41.0 33.0 41.0 29 37.534832204134986 40.0 36.0 41.0 32.0 41.0 30 37.55218687504903 40.0 36.0 41.0 32.0 41.0 31 37.528015566506255 40.0 36.0 41.0 32.0 41.0 32 37.44964939834476 40.0 36.0 41.0 32.0 41.0 33 37.42880869450657 40.0 36.0 41.0 32.0 41.0 34 37.36938034469538 40.0 36.0 41.0 32.0 41.0 35 37.333689584681 39.0 36.0 41.0 32.0 41.0 36 37.22457749428986 39.0 36.0 41.0 31.0 41.0 37 37.183920432593744 39.0 36.0 41.0 31.0 41.0 38 37.12636980370156 39.0 36.0 41.0 31.0 41.0 39 37.01469462795665 39.0 35.0 41.0 31.0 41.0 40 36.93722180199037 39.0 35.0 41.0 31.0 41.0 41 36.85018495958126 39.0 35.0 41.0 31.0 41.0 42 36.77496510266404 39.0 35.0 40.0 31.0 41.0 43 36.69075779398508 39.0 35.0 40.0 31.0 41.0 44 36.632744581106145 39.0 35.0 40.0 31.0 41.0 45 36.47061444475859 39.0 35.0 40.0 30.0 41.0 46 36.308287432666276 39.0 35.0 40.0 30.0 41.0 47 36.191735511499466 38.0 35.0 40.0 30.0 41.0 48 36.10691685182362 38.0 35.0 40.0 29.0 41.0 49 35.941091964657105 38.0 35.0 40.0 29.0 41.0 50 35.851251493221106 38.0 35.0 40.0 29.0 41.0 51 34.66078013124063 37.0 33.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 6.0 10 7.0 11 13.0 12 13.0 13 24.0 14 35.0 15 48.0 16 98.0 17 200.0 18 385.0 19 625.0 20 1091.0 21 1700.0 22 2453.0 23 3497.0 24 4596.0 25 5968.0 26 7594.0 27 9968.0 28 12873.0 29 16739.0 30 22222.0 31 29349.0 32 38527.0 33 52562.0 34 77326.0 35 98639.0 36 122732.0 37 182184.0 38 292223.0 39 367290.0 40 119.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.203078070854545 32.30730971515188 20.916715638891397 26.572896575102174 2 20.867274662387704 29.770351104946613 22.161103569964162 27.20127066270152 3 20.841814039757057 29.112889736260517 22.606590452562568 27.43870577141986 4 19.88889102705487 29.23079314280301 21.192193654679944 29.68812217546218 5 17.983711122591416 33.13004309062354 20.51297233525719 28.373273451527858 6 20.067263412345145 34.304044242272624 21.872229121919375 23.756463223462852 7 84.91606136010054 4.698225009732767 5.682603733533861 4.703109896632832 8 87.26843045623363 3.0599375622638045 4.133576492147915 5.538055489354647 9 79.2271664843469 6.530871745074035 8.560172184861884 5.6817895857171825 10 33.54999533715342 27.79174987010642 15.557106548264905 23.10114824447527 11 22.237707478169735 25.54211142575046 31.683598474138964 20.53658262194084 12 20.769576924386392 20.870383226778657 38.87385593728398 19.48618391155098 13 23.903009830464818 23.9572616804307 30.775157537602528 21.364570951501953 14 19.857657356269602 27.941108987747814 30.668726213931404 21.53250744205118 15 19.320319797262393 33.293316734586334 28.435000658719595 18.95136280943168 16 19.824573349537342 30.84162160481857 28.57954890289881 20.754256142745277 17 18.14217389309203 31.726970348736515 30.307910704267467 19.822945053903986 18 17.571974367666193 31.75694579107783 30.05981766049444 20.61126218076154 19 17.710823577128664 34.92642324140371 30.374966878986548 16.987786302481076 20 18.37953498837249 32.874918770251924 30.044274838539685 18.701271402835896 21 18.37635241054366 32.78521448354163 29.433145881966325 19.40528722394838 22 16.66294132362672 32.11169219883562 30.796621434587664 20.42874504295 23 17.297236486256445 31.511443217630593 28.891145476372692 22.300174819740274 24 15.563841771111964 32.995560673995975 29.86952911170552 21.571068443186544 25 17.560206231043306 31.109846303694898 29.177133400340168 22.15281406492163 26 18.40307126161826 32.4705093456768 29.247816233515355 19.87860315918958 27 16.0260556906712 31.627052207598812 30.099784916949524 22.247107184780468 28 18.12485474862816 30.75517390937499 30.236117669524077 20.883853672472775 29 18.327799595294522 32.7545729202594 29.241451077857693 19.67617640658838 30 18.45473264125835 32.66479462011122 27.723879547570657 21.15659319105977 31 17.00769591727074 32.29110077225621 29.24418957505925 21.457013735413803 32 18.701493443149538 32.52342895376085 28.379712620623398 20.395364982466216 33 19.060458616866477 29.665178009719444 30.489169613635053 20.785193759779023 34 15.659467132852642 30.289481358235403 31.21146675390384 22.83958475500812 35 18.727250119531703 30.130426480231755 30.984689580240186 20.15763381999636 36 17.40766453557308 31.586936924267974 31.757685925456624 19.247712614702326 37 18.40011072410307 32.47346988319199 29.238342513466748 19.888076879238195 38 19.705855795178174 31.57450266670417 29.64141969616004 19.078221841957628 39 18.987259326803375 31.11621145935256 29.027108161757848 20.869421052086217 40 21.110630846136424 29.987654558561655 28.154415715717345 20.747298879584577 41 18.762702556276118 29.088761355511707 30.432179266467617 21.716356821744558 42 19.706003822053933 30.584054840996934 28.74889164876775 20.961049688181387 43 17.683956699178303 29.115258166272667 31.650144400217307 21.550640734331726 44 19.346816608023353 28.679911124663793 29.700038338960823 22.27323392835203 45 20.47529949537638 29.062338558188628 30.506414744661043 19.955947201773956 46 17.3673272119286 30.31094525522054 31.68537479664808 20.636352736202785 47 19.08443897073953 29.697299841759268 29.221837516819555 21.99642367068165 48 18.62703592464248 28.748151514388955 31.920515488792145 20.704297072176423 49 18.950178594425605 28.680059151539556 30.4531250693876 21.916637184647243 50 17.692394231096596 28.467862625138217 29.52640281369485 24.313340330070325 51 18.090290473138303 27.539438060374245 32.278074407189365 22.092197059298087 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2122.0 1 1689.0 2 1256.0 3 946.0 4 636.0 5 824.0 6 1012.0 7 1070.0 8 1128.0 9 1257.5 10 1387.0 11 1680.0 12 1973.0 13 2129.5 14 2286.0 15 2591.5 16 2897.0 17 3534.0 18 4171.0 19 5560.0 20 6949.0 21 8787.5 22 10626.0 23 12691.0 24 14756.0 25 18849.5 26 27910.0 27 32877.0 28 37940.0 29 43003.0 30 48641.5 31 54280.0 32 58576.5 33 62873.0 34 66700.5 35 70528.0 36 74360.0 37 78192.0 38 79598.0 39 81004.0 40 79638.0 41 78272.0 42 80024.5 43 81777.0 44 91600.5 45 101424.0 46 105685.5 47 109947.0 48 112446.0 49 114945.0 50 106715.5 51 98486.0 52 85508.5 53 72531.0 54 61126.5 55 49722.0 56 43001.0 57 36280.0 58 32564.5 59 28849.0 60 26083.0 61 23317.0 62 20782.5 63 18248.0 64 15388.0 65 12528.0 66 11265.0 67 10002.0 68 8081.5 69 6161.0 70 4853.5 71 3546.0 72 2998.0 73 2450.0 74 2234.5 75 1892.0 76 1765.0 77 1361.5 78 958.0 79 679.5 80 401.0 81 347.5 82 294.0 83 235.5 84 177.0 85 120.5 86 64.0 87 42.5 88 21.0 89 16.0 90 11.0 91 9.0 92 7.0 93 5.5 94 4.0 95 2.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1351106.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.225659333625348 #Duplication Level Percentage of deduplicated Percentage of total 1 73.57352423573997 21.502347552879648 2 10.143748872408883 5.929154978217365 3 3.8083835870571905 3.3390756398131063 4 2.152409277148719 2.51622321121933 5 1.3519513219335013 1.9755834385236481 6 1.0065395916189621 1.7650069926277299 7 0.7825540526565968 1.600946070716273 8 0.6221888606097603 1.4547103745085892 9 0.5349008847931042 1.406954793259624 >10 5.036108679273058 31.09142668766287 >50 0.7486903588455558 15.241490329696655 >100 0.233127407871783 9.410491200349657 >500 0.0035747904608639464 0.7202100676584462 >1k 0.0017873952304319732 1.184380269796622 >5k 5.106843515519922E-4 0.8619983930704291 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGCT 6069 0.4491875544923936 No Hit AATCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCT 5482 0.4057416664569619 No Hit AATGATACGGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCC 4592 0.33986970674395645 No Hit AATGATACCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGT 4476 0.3312841479499018 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1650 0.1221221725016394 No Hit CCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC 1595 0.11805143341825143 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.480268757595629E-4 0.0 0.0 0.5207585489221424 0.0 2 1.480268757595629E-4 0.0 0.0 0.8179225020094648 0.0 3 1.480268757595629E-4 0.0 0.0 1.2296592569346891 0.0 4 1.480268757595629E-4 0.0 0.0 2.8219103460424275 0.0 5 2.2204031363934435E-4 0.0 0.0 3.0908751792975533 0.0 6 2.2204031363934435E-4 0.0 0.0 4.552566563985357 0.0 7 2.2204031363934435E-4 0.0 0.0 5.654034546512265 0.0 8 2.2204031363934435E-4 0.0 0.0 6.697920074368702 0.0 9 2.2204031363934435E-4 0.0 0.0 8.169381232856637 0.0 10 2.2204031363934435E-4 0.0 0.0 10.056279818163786 0.0 11 2.2204031363934435E-4 0.0 0.0 11.877380457195809 0.0 12 2.2204031363934435E-4 0.0 0.0 12.632539563883219 0.0 13 2.2204031363934435E-4 0.0 0.0 12.962195416199766 0.0 14 2.2204031363934435E-4 0.0 0.0 13.273717976235766 0.0 15 2.2204031363934435E-4 0.0 0.0 13.511967232770782 0.0 16 2.2204031363934435E-4 0.0 0.0 13.99682926432123 0.0 17 2.2204031363934435E-4 0.0 0.0 14.720458646471853 0.0 18 2.2204031363934435E-4 0.0 0.0 15.553035809181514 0.0 19 2.2204031363934435E-4 0.0 0.0 15.97439431103111 0.0 20 2.2204031363934435E-4 0.0 0.0 16.37465898308497 0.0 21 2.2204031363934435E-4 0.0 0.0 16.81429880409087 0.0 22 2.2204031363934435E-4 0.0 0.0 17.31633195322943 0.0 23 2.2204031363934435E-4 0.0 0.0 17.81385028265732 0.0 24 2.2204031363934435E-4 0.0 0.0 18.224550849452225 0.0 25 2.2204031363934435E-4 0.0 0.0 18.5855883994298 0.0 26 2.2204031363934435E-4 0.0 0.0 18.910507391722042 0.0 27 2.2204031363934435E-4 0.0 0.0 19.27021269981778 0.0 28 2.2204031363934435E-4 0.0 0.0 19.620444287864906 0.0 29 2.2204031363934435E-4 0.0 0.0 19.99806084792755 0.0 30 2.2204031363934435E-4 0.0 0.0 20.55597414266534 0.0 31 2.960537515191258E-4 0.0 0.0 20.906353757588228 0.0 32 2.960537515191258E-4 0.0 0.0 21.24022837586392 0.0 33 2.960537515191258E-4 0.0 0.0 21.60008171083542 0.0 34 2.960537515191258E-4 0.0 0.0 21.941135632585453 0.0 35 2.960537515191258E-4 0.0 0.0 22.28759253530071 0.0 36 2.960537515191258E-4 0.0 0.0 22.634049438015968 0.0 37 2.960537515191258E-4 0.0 0.0 22.98250470355398 0.0 38 2.960537515191258E-4 0.0 0.0 23.335622815678416 0.0 39 2.960537515191258E-4 0.0 0.0 23.70324756162729 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGGGTC 240 0.0 40.312496 5 ACGGGCC 580 0.0 39.956894 5 CTATGCG 70 0.0 38.571426 1 TCGCGCG 100 0.0 38.25 1 CGCTGCG 95 0.0 37.894737 1 AGGGCGT 485 0.0 37.113403 6 TAGGGAG 735 0.0 37.040817 5 CATGGGA 875 0.0 37.02857 4 TGCGGGC 530 0.0 36.509434 4 GTACGGG 475 0.0 36.473682 3 TAGGGAC 685 0.0 36.459854 5 TACTAGG 340 0.0 36.39706 2 ACTATGG 330 0.0 36.136364 2 CGAGGGC 430 0.0 36.104652 4 ACAGGGC 885 0.0 36.101696 4 CCTAGGG 900 0.0 36.0 3 ACGTGTC 25 0.0021072396 36.0 13 AACGGGC 745 0.0 35.637585 4 CAGGGAT 1470 0.0 35.510204 5 TATGGGC 660 0.0 35.454544 4 >>END_MODULE