##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553145_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1022250
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.713290291024702	31.0	31.0	34.0	30.0	34.0
2	31.91818831010027	33.0	31.0	34.0	30.0	34.0
3	32.074667644900956	34.0	31.0	34.0	30.0	34.0
4	35.75007287845439	37.0	35.0	37.0	35.0	37.0
5	35.52384348251406	37.0	35.0	37.0	33.0	37.0
6	35.561973098557104	37.0	35.0	37.0	33.0	37.0
7	35.77262117877232	37.0	35.0	37.0	35.0	37.0
8	35.85658302763512	37.0	35.0	37.0	35.0	37.0
9	37.53079775006114	39.0	37.0	39.0	35.0	39.0
10	37.00639373930056	39.0	37.0	39.0	33.0	39.0
11	36.97543947175348	39.0	37.0	39.0	33.0	39.0
12	37.01002103203717	39.0	37.0	39.0	33.0	39.0
13	37.01641672780631	39.0	37.0	39.0	33.0	39.0
14	38.19749376375642	40.0	38.0	41.0	33.0	41.0
15	38.17847297627782	40.0	37.0	41.0	33.0	41.0
16	38.083365125947665	40.0	37.0	41.0	33.0	41.0
17	38.05342626559061	40.0	37.0	41.0	33.0	41.0
18	37.99321105404744	40.0	37.0	41.0	33.0	41.0
19	38.07041721692345	40.0	37.0	41.0	33.0	41.0
20	37.982281242357544	40.0	37.0	41.0	33.0	41.0
21	37.96981364636831	40.0	37.0	41.0	33.0	41.0
22	37.932743458058205	40.0	37.0	41.0	33.0	41.0
23	37.82361848862803	40.0	37.0	41.0	33.0	41.0
24	37.711249694301785	40.0	37.0	41.0	32.0	41.0
25	37.75617803864026	40.0	37.0	41.0	33.0	41.0
26	37.70477573978968	40.0	37.0	41.0	33.0	41.0
27	37.65639520665199	40.0	37.0	41.0	33.0	41.0
28	37.59734213744192	40.0	37.0	41.0	32.0	41.0
29	37.424436292492054	40.0	36.0	41.0	32.0	41.0
30	37.45764441183663	40.0	36.0	41.0	32.0	41.0
31	37.3790892638787	39.0	36.0	41.0	32.0	41.0
32	37.34263047199804	39.0	36.0	41.0	32.0	41.0
33	37.292754218635366	39.0	36.0	41.0	31.0	41.0
34	37.23846515040352	39.0	36.0	41.0	31.0	41.0
35	37.18823379799462	39.0	36.0	41.0	31.0	41.0
36	37.08237319638053	39.0	36.0	41.0	31.0	41.0
37	37.03562142333089	39.0	36.0	41.0	31.0	41.0
38	37.004993886035706	39.0	36.0	41.0	31.0	41.0
39	36.93410907312301	39.0	35.0	41.0	31.0	41.0
40	36.82149963316214	39.0	35.0	41.0	31.0	41.0
41	36.752165321594525	39.0	35.0	41.0	31.0	41.0
42	36.703807287845436	39.0	35.0	40.0	31.0	41.0
43	36.64086573734409	39.0	35.0	40.0	30.0	41.0
44	36.58660503790658	39.0	35.0	40.0	30.0	41.0
45	36.4110892638787	39.0	35.0	40.0	30.0	41.0
46	36.28636537050624	39.0	35.0	40.0	30.0	41.0
47	36.1474081682563	38.0	35.0	40.0	29.0	41.0
48	36.049828319882614	38.0	35.0	40.0	29.0	41.0
49	35.90345023233064	38.0	35.0	40.0	29.0	41.0
50	35.79679236977256	38.0	35.0	40.0	28.0	41.0
51	34.63336170212766	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	4.0
11	9.0
12	11.0
13	17.0
14	23.0
15	42.0
16	93.0
17	129.0
18	287.0
19	478.0
20	838.0
21	1367.0
22	1965.0
23	2776.0
24	3717.0
25	4732.0
26	6024.0
27	7834.0
28	10326.0
29	13480.0
30	17360.0
31	23057.0
32	29922.0
33	39962.0
34	57245.0
35	73558.0
36	95347.0
37	140360.0
38	219959.0
39	271246.0
40	79.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.375103937393007	31.74194179505992	22.10310589386158	26.7798483736855
2	20.57128882367327	29.072046955245785	22.202592320860845	28.154071900220103
3	20.07062851552947	29.242553191489364	22.915040352164343	27.77177794081683
4	19.21252139887503	29.494057226705795	21.438884812912693	29.854536561506478
5	18.03844460748349	34.13225727561751	19.687747615553924	28.14155050134507
6	20.317241379310342	34.43883590119834	20.811836634874055	24.432086084617268
7	85.72824651504035	3.989924186842749	5.608999755441428	4.672829542675471
8	88.27791636096846	2.687307410124725	4.046368305209098	4.988407923697726
9	79.97710931768158	6.18870139398386	8.575201760821717	5.258987527512839
10	31.706529713866473	30.59535338713622	15.446515040352166	22.251601858645145
11	19.97906578625581	25.522915138175595	34.32761066275373	20.17040841281487
12	19.298606016140866	20.67263389581805	40.3448275862069	19.68393250183419
13	23.19158718513084	23.725018341892884	31.982978723404255	21.100415749572022
14	19.557055514795792	29.122328197603327	30.20298361457569	21.117632673025188
15	17.94942528735632	34.89616043042309	28.46681340180973	18.68760088041086
16	18.692296404988994	33.393396918562	27.807385668867695	20.106921007581317
17	16.988114453411594	33.58992418684275	30.180777696258254	19.241183663487405
18	17.205478112007828	32.9855710442651	30.24260210320372	19.566348740523356
19	17.00679872829543	35.93064318904378	30.223820004891174	16.838738077769626
20	17.18982636341404	34.256590853509415	30.327904133039862	18.225678650036684
21	17.338517975055026	34.8778674492541	29.514795793592562	18.26881878209831
22	15.854732208363902	33.275519686965026	30.83257520176082	20.037172902910246
23	16.446270481780388	32.65844949865493	29.669258987527513	21.226021032037174
24	14.303839569576915	33.644020542920025	30.834336023477622	21.217803864025434
25	16.32692589875275	33.680215211543164	29.44631939349474	20.54653949620934
26	17.74732208363903	34.36087062851553	28.639569576913672	19.25223771093177
27	15.923208608461724	33.47253607238934	28.532061628760086	22.072193690388847
28	17.501491807287845	32.68760088041086	28.97089752995842	20.84000978234287
29	17.17084861824407	34.88530202983615	26.93812668134018	21.005722670579605
30	17.295280019564686	34.94605037906579	26.45135730007337	21.30731230129616
31	16.07160674981658	35.767375886524825	25.54512105649303	22.615896307165567
32	16.55221325507459	34.220983125458545	27.10266568843238	22.124137931034483
33	16.421423330887748	33.01100513573001	28.441966250917094	22.12560528246515
34	14.332795304475422	32.9627781853754	28.610711665443873	24.093714844705307
35	16.442651014918074	32.56708241623869	28.9610173636586	22.02924920518464
36	16.014282220591834	34.848324773783325	28.98067987282954	20.156713132795304
37	16.329762778185376	33.88026412325752	28.22910247004157	21.56087062851553
38	17.31386647101981	32.823477622890685	29.342626559060896	20.52002934702861
39	17.516752262166786	32.655319148936165	29.222695035460994	20.605233553436047
40	18.201614086573734	33.788114453411595	28.632428466617753	19.377842993396918
41	16.07160674981658	31.889948642699927	32.09870383956957	19.939740767913918
42	17.107948153582782	32.57285399853265	30.513475177304965	19.805722670579602
43	15.957055514795792	32.190364392271945	30.882171680117388	20.97040841281487
44	18.132746392761064	31.07097089752996	28.83492296404989	21.961359745659085
45	18.28681829298117	31.207336757153335	29.80190755685987	20.703937393005624
46	16.58126681340181	31.553338224504767	30.851944240645636	21.01345072144779
47	17.423330887747614	31.422254830031793	29.06118855465884	22.09322572756175
48	17.07957935925654	30.860259232086086	31.485057471264366	20.575103937393006
49	18.08901932012717	29.658107116654435	29.90794815358278	22.344925409635607
50	16.756175103937395	29.640401076057714	29.24822695035461	24.355196869650282
51	17.096698459280997	29.06950354609929	31.517632673025194	22.31616532159452
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1199.0
1	987.5
2	776.0
3	625.0
4	474.0
5	679.5
6	885.0
7	926.5
8	968.0
9	1092.0
10	1216.0
11	1573.0
12	1930.0
13	2303.5
14	2677.0
15	3038.5
16	3400.0
17	3850.0
18	4300.0
19	5256.5
20	6213.0
21	7382.0
22	8551.0
23	10660.0
24	12769.0
25	15934.5
26	22457.0
27	25814.0
28	29905.0
29	33996.0
30	38692.5
31	43389.0
32	47865.0
33	52341.0
34	55241.5
35	58142.0
36	59425.0
37	60708.0
38	62137.5
39	63567.0
40	61916.0
41	60265.0
42	61413.0
43	62561.0
44	69476.0
45	76391.0
46	80417.0
47	84443.0
48	89486.5
49	94530.0
50	87622.0
51	80714.0
52	68582.5
53	56451.0
54	46550.5
55	36650.0
56	29693.5
57	22737.0
58	18754.0
59	14771.0
60	13001.0
61	11231.0
62	9239.0
63	7247.0
64	6057.5
65	4868.0
66	3947.5
67	3027.0
68	2290.5
69	1554.0
70	1245.5
71	937.0
72	772.0
73	607.0
74	469.0
75	308.0
76	285.0
77	195.5
78	106.0
79	80.0
80	54.0
81	44.5
82	35.0
83	24.5
84	14.0
85	13.0
86	12.0
87	9.0
88	6.0
89	5.0
90	4.0
91	4.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1022250.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.080704926440504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.8055330654497	26.996804886121474
2	9.69028593006799	7.1864526645137845
3	4.111041436052984	4.573209432919528
4	2.4745151429825927	3.67027063411785
5	1.6937890628406387	3.1403446223412956
6	1.2740618810126507	2.834586760071351
7	1.0348360222096689	2.686071443076572
8	0.8694256891532566	2.5791133947967273
9	0.6792561064552652	2.26685657276555
>10	5.15257235803234	35.84536813741712
>50	0.17956880077669785	4.261048572568161
>100	0.03139023929359644	2.147627350667102
>500	0.0023941707895893194	0.6037859177391688
>1k	0.0013300948831051777	1.20845961088426
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGCT	4919	0.48119344583027635	No Hit
AATCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCT	2455	0.2401565174859379	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCC	1866	0.18253851797505502	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGT	1846	0.1805820494008315	No Hit
CCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGC	1165	0.11396429444852042	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5613108339447298	0.0
2	0.0	0.0	0.0	0.8755196869650281	0.0
3	0.0	0.0	0.0	1.2539985326485694	0.0
4	0.0	0.0	0.0	2.7125458547322086	0.0
5	0.0	0.0	0.0	3.0337979946197113	0.0
6	0.0	0.0	0.0	4.73680606505258	0.0
7	0.0	0.0	0.0	6.070237221814625	0.0
8	0.0	0.0	0.0	7.513817559305454	0.0
9	0.0	0.0	0.0	9.085742235265347	0.0
10	0.0	0.0	0.0	11.265346050379065	0.0
11	0.0	0.0	0.0	13.02616776718024	0.0
12	0.0	0.0	0.0	13.845732452922475	0.0
13	0.0	0.0	0.0	14.246808510638298	0.0
14	0.0	0.0	0.0	14.585864514551234	0.0
15	0.0	0.0	0.0	14.880899975544143	0.0
16	0.0	0.0	0.0	15.393396918561995	0.0
17	0.0	0.0	0.0	16.139789679628272	0.0
18	0.0	0.0	0.0	17.078307654683297	0.0
19	0.0	0.0	0.0	17.50618733186598	0.0
20	0.0	0.0	0.0	17.99686965028124	0.0
21	0.0	0.0	0.0	18.45898752751284	0.0
22	0.0	0.0	0.0	18.984886280264124	0.0
23	0.0	0.0	0.0	19.52849107361213	0.0
24	0.0	0.0	0.0	19.983663487405234	0.0
25	0.0	0.0	0.0	20.381511371973588	0.0
26	0.0	0.0	0.0	20.736414771337735	0.0
27	9.782342871117633E-5	0.0	0.0	21.16840303252629	0.0
28	9.782342871117633E-5	0.0	0.0	21.55774027879677	0.0
29	9.782342871117633E-5	0.0	0.0	21.964392271949134	0.0
30	9.782342871117633E-5	0.0	0.0	22.592027390560038	0.0
31	9.782342871117633E-5	0.0	0.0	22.980679872829544	0.0
32	9.782342871117633E-5	0.0	0.0	23.360723893372462	0.0
33	9.782342871117633E-5	0.0	0.0	23.744093910491564	0.0
34	9.782342871117633E-5	0.0	0.0	24.118659819026657	0.0
35	9.782342871117633E-5	0.0	0.0	24.511910002445585	0.0
36	9.782342871117633E-5	0.0	0.0	24.898899486427	0.0
37	9.782342871117633E-5	0.0	0.0	25.27649792125214	0.0
38	9.782342871117633E-5	0.0	0.0	25.660943996087063	0.0
39	9.782342871117633E-5	0.0	0.0	26.061922230374176	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCAG	50	2.1827873E-11	45.0	1
CCTCACG	90	0.0	42.5	1
CGCATAG	65	0.0	41.538464	1
CCACGCG	60	3.6379788E-12	41.250004	1
CCTCGCG	50	1.0804797E-9	40.5	1
CTCACGG	290	0.0	39.56897	2
ACCGCGG	270	0.0	38.333332	2
CAACCCG	100	0.0	38.25	1
AACCGCG	190	0.0	37.894737	1
ACAGGGC	855	0.0	37.63158	4
CCCAGCG	60	1.546141E-10	37.500004	1
CCCGTAG	30	1.1398334E-4	37.500004	1
TCGCGCG	30	1.1398334E-4	37.500004	1
TAGGGAC	550	0.0	37.227272	5
AGGGCGT	370	0.0	37.094593	6
GGGCGTC	250	0.0	36.899998	7
TCTCGCG	55	2.748493E-9	36.818184	1
CGTACAG	55	2.748493E-9	36.818184	1
CAGGGCA	765	0.0	36.764706	5
ATGGGCC	460	0.0	36.68478	5
>>END_MODULE