FastQCFastQC Report
Sat 18 Jun 2016
SRR3553142_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553142_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1351576
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCT64640.47825649464033104No Hit
AATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCT27110.20058065547183435No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCC20610.15248865028677633No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGT19900.1472355235665623No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14060.10402670660029475No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT351.2121382E-745.00000420
CATAGCG501.0822987E-940.51
CTAACGG3450.040.4347842
TCGGACG451.9286745E-840.01
CACTACG451.9286745E-840.01
CGTTAGG1700.039.7058832
TAACGGG8950.038.4636883
ATAGGGA9050.038.2872964
GTAGGGT3350.038.2835854
CAGGGAA17650.036.9688385
ACGGGTC3350.036.94035
CGGGTCA1950.036.9230776
CCAGGGA15500.036.8709684
ATGGGAC6350.036.8503955
GATAGGG7950.036.5094343
CATACGG2600.036.3461572
AACGGGA11350.036.2775344
CTAGGGC8600.036.1046524
AACGGGC7550.036.0596054
GCGAGAC1000.036.021
Produced by FastQC (version 0.11.5)