##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553142_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1351576
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.762791733502223	31.0	31.0	34.0	30.0	34.0
2	31.971932026019996	33.0	31.0	34.0	30.0	34.0
3	32.1384354264947	34.0	31.0	34.0	30.0	34.0
4	35.79868686629535	37.0	35.0	37.0	35.0	37.0
5	35.569984225822296	37.0	35.0	37.0	33.0	37.0
6	34.86405721912789	37.0	35.0	37.0	32.0	37.0
7	35.37444879163288	37.0	35.0	37.0	33.0	37.0
8	35.81304195990459	37.0	35.0	37.0	35.0	37.0
9	37.48767216937856	39.0	37.0	39.0	35.0	39.0
10	37.00757412087815	39.0	37.0	39.0	33.0	39.0
11	36.984188828449156	39.0	37.0	39.0	33.0	39.0
12	36.878893972665985	39.0	37.0	39.0	33.0	39.0
13	36.86762786554363	39.0	37.0	39.0	33.0	39.0
14	38.04543732649884	40.0	37.0	41.0	33.0	41.0
15	38.02817969540744	40.0	37.0	41.0	33.0	41.0
16	37.944660159695054	40.0	37.0	41.0	33.0	41.0
17	37.95547642159967	40.0	37.0	41.0	33.0	41.0
18	37.93766240300212	40.0	37.0	41.0	33.0	41.0
19	37.98706473035922	40.0	37.0	41.0	33.0	41.0
20	37.89426491740013	40.0	37.0	41.0	33.0	41.0
21	37.90139141269155	40.0	37.0	41.0	33.0	41.0
22	37.883874084772145	40.0	37.0	41.0	33.0	41.0
23	37.800380444754865	40.0	37.0	41.0	33.0	41.0
24	37.640032820943844	39.0	36.0	41.0	32.0	41.0
25	37.69481849337366	40.0	36.0	41.0	33.0	41.0
26	37.61604970789656	40.0	36.0	41.0	33.0	41.0
27	37.54339230646298	39.0	36.0	41.0	32.0	41.0
28	37.51090948640698	39.0	36.0	41.0	32.0	41.0
29	37.300754082641305	39.0	36.0	41.0	32.0	41.0
30	37.388983675353806	39.0	36.0	41.0	32.0	41.0
31	37.3500247118919	39.0	36.0	41.0	32.0	41.0
32	37.30967625941864	39.0	36.0	41.0	32.0	41.0
33	37.132660686487476	39.0	36.0	41.0	31.0	41.0
34	37.149031205052474	39.0	36.0	41.0	31.0	41.0
35	37.1018966007091	39.0	35.0	41.0	31.0	41.0
36	37.080789389571876	39.0	35.0	41.0	31.0	41.0
37	37.048180050548396	39.0	35.0	41.0	31.0	41.0
38	36.89437885845857	39.0	35.0	41.0	31.0	41.0
39	36.867823932949385	39.0	35.0	41.0	31.0	41.0
40	36.81363016212185	39.0	35.0	41.0	31.0	41.0
41	36.751805299886946	39.0	35.0	40.0	31.0	41.0
42	36.64912664918584	39.0	35.0	40.0	31.0	41.0
43	36.55729089596146	39.0	35.0	40.0	31.0	41.0
44	36.483173717201254	39.0	35.0	40.0	30.0	41.0
45	36.310738722794724	38.0	35.0	40.0	30.0	41.0
46	36.19048429389098	38.0	35.0	40.0	30.0	41.0
47	36.09143991902786	38.0	35.0	40.0	30.0	41.0
48	35.98621239205195	38.0	35.0	40.0	29.0	41.0
49	35.88123864288801	38.0	35.0	40.0	29.0	41.0
50	35.78150766216624	38.0	34.0	40.0	29.0	41.0
51	34.585051081108276	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	1.0
10	13.0
11	5.0
12	7.0
13	24.0
14	32.0
15	50.0
16	94.0
17	198.0
18	352.0
19	625.0
20	1179.0
21	1983.0
22	2833.0
23	3761.0
24	4720.0
25	6293.0
26	8038.0
27	10511.0
28	13436.0
29	17703.0
30	23706.0
31	30664.0
32	40575.0
33	55801.0
34	83892.0
35	105184.0
36	125611.0
37	185326.0
38	282649.0
39	346192.0
40	115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.73185377662817	31.344149348612287	22.63083984918347	26.29315702557607
2	22.282357780842514	29.48631819446335	21.45510130395923	26.776222720734904
3	21.41596181050862	28.78987197168343	22.346061190787644	27.448105027020308
4	20.50317555209622	30.234777770543424	20.591664841636725	28.670381835723628
5	18.41849810887438	33.29735064842821	19.41378065310423	28.870370589593186
6	19.987407293411543	37.15551326747442	20.076266521453473	22.78081291766057
7	85.44743321870172	4.522646155303142	5.377869982894044	4.6520506431010915
8	87.93571356697663	3.202261656022303	4.098030743369222	4.76399403363185
9	80.0617205395775	6.4392975311784175	8.147673530752247	5.351308398491835
10	36.26521926994856	28.072931155924643	14.450907681107092	21.210941893019704
11	25.548766773011657	27.1966208337526	26.78339952766252	20.471212865573225
12	23.771878162974186	21.412854327096664	34.95119771289221	19.86406979703694
13	25.49712335821293	24.132124275660413	28.517005333033435	21.853747033093217
14	21.61846614618786	27.545102902093554	28.43384315791343	22.402587793805157
15	19.721347523187745	31.82906473627824	28.032459883868903	20.417127856665108
16	21.79070951245065	28.913727381960026	26.659840068187062	22.63572303740226
17	19.9464920951541	29.67483885478878	28.32315755828751	22.055511491769607
18	20.41601804116084	29.442295512793955	28.697831272529257	21.443855173515956
19	20.18961567828964	33.02781345629103	28.118803530101154	18.663767335318177
20	20.855283017751127	30.993447649262784	28.129383771241866	20.021885561744217
21	20.73201950907681	30.108628741558004	28.516117480630022	20.643234268735167
22	19.68790508265906	29.10106423908089	28.870000651091765	22.341030027168284
23	20.021145684741366	29.112976258826734	27.84393922354348	23.02193883288842
24	18.611384043516605	30.273695300893177	28.332036082321675	22.78288457326854
25	20.26774668979029	29.135690482814137	27.44011435538956	23.156448472006012
26	20.863569640183016	30.106779049050886	27.069140026162053	21.96051128460405
27	18.516975737953324	28.73763665528243	28.797788655613893	23.94759895115036
28	19.48628859938324	28.997111520180884	29.156702989694992	22.35989689074088
29	19.709361515741623	31.530524365629457	26.85982882205662	21.900285296572296
30	20.171858630221312	29.138871953926383	28.012261241691185	22.677008174161127
31	19.49561104961911	30.44275719604373	26.408429862619638	23.653201891717522
32	19.631156516540692	29.127995761984526	28.210400303053618	23.03044741842116
33	19.484660869976974	28.235630108850707	30.231670287131468	22.048038734040855
34	17.63940762487644	27.56759516298011	28.820946805803004	25.97205040634045
35	19.167179648055306	27.685975483435634	30.463103813622023	22.683741054887037
36	18.572096574665427	29.191477208828804	30.673598820932007	21.562827395573763
37	19.33883111271582	29.61032158014052	28.601573274458854	22.44927403268481
38	20.129611653358744	27.712167129336418	30.16197387346328	21.99624734384156
39	18.985835794657497	27.848526460961132	29.12252067216346	24.043117072217914
40	20.481793106713937	27.538148058266792	28.109333104464714	23.870725730554554
41	19.40741771087974	27.37766873634927	29.214339408216777	24.00057414455421
42	20.75081238494913	28.275657454704728	27.97778297335851	22.995747186987636
43	18.64231090223561	27.56041835605249	29.905828455077625	23.89144228663427
44	20.07352897654294	26.230489443434923	29.654566224910774	24.04141535511137
45	20.82827750714721	26.17004149230232	30.39007795344102	22.611603047109448
46	18.283026629653087	27.508552978152913	31.16265751981391	23.045762872380095
47	19.279197026286347	27.534596648653125	29.092851604349296	24.09335472071123
48	19.705440167626534	26.200968351021324	31.230948167176685	22.86264331417545
49	20.435624781736283	26.10796581176345	29.677132473497608	23.779276933002656
50	19.451810331050567	25.871353146252968	29.020713596571706	25.656122926124763
51	19.613251493071793	25.099883395384353	31.6812373111094	23.605627800434455
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1742.0
1	1393.5
2	1045.0
3	736.0
4	427.0
5	523.0
6	619.0
7	648.5
8	678.0
9	763.5
10	849.0
11	1144.0
12	1439.0
13	1600.5
14	1762.0
15	2037.0
16	2312.0
17	3021.5
18	3731.0
19	4141.5
20	4552.0
21	5872.5
22	7193.0
23	9006.0
24	10819.0
25	14061.5
26	20906.5
27	24509.0
28	28539.0
29	32569.0
30	37699.0
31	42829.0
32	46206.5
33	49584.0
34	52830.5
35	56077.0
36	59568.0
37	63059.0
38	65228.0
39	67397.0
40	68061.5
41	68726.0
42	71395.5
43	74065.0
44	80765.5
45	87466.0
46	97113.0
47	106760.0
48	112290.0
49	117820.0
50	111505.5
51	105191.0
52	94005.5
53	82820.0
54	73627.5
55	64435.0
56	58728.5
57	53022.0
58	49512.5
59	46003.0
60	43010.0
61	40017.0
62	35936.0
63	31855.0
64	28392.0
65	24929.0
66	21638.0
67	18347.0
68	15846.0
69	13345.0
70	11417.0
71	9489.0
72	7796.5
73	6104.0
74	4887.5
75	3271.0
76	2871.0
77	2176.5
78	1482.0
79	1355.5
80	1229.0
81	1020.0
82	811.0
83	578.0
84	345.0
85	255.5
86	166.0
87	104.5
88	43.0
89	40.5
90	38.0
91	29.0
92	20.0
93	12.5
94	5.0
95	4.0
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1351576.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.23825452218307
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.08705888345614	20.920892255509987
2	10.19840152982438	5.759701162372041
3	3.831906175442624	3.2461902566182173
4	2.022861129922705	2.284882697991527
5	1.2719618688819454	1.7958991498000014
6	0.9221358721301967	1.5623704476748652
7	0.6852035864534143	1.354426729164893
8	0.5557969374734923	1.2555788306421078
9	0.4647422277092076	1.181115838193307
>10	4.710477915475559	29.311582755925464
>50	0.9711466776327424	19.138871461625882
>100	0.27322273722328577	10.271141216719213
>500	0.0032112368680271004	0.6112569062665507
>1k	0.0016056184340135502	0.8176288448268115
>5k	2.676030723355917E-4	0.4884614466691784
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCT	6464	0.47825649464033104	No Hit
AATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCT	2711	0.20058065547183435	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCC	2061	0.15248865028677633	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGT	1990	0.1472355235665623	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1406	0.10402670660029475	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.398770028470468E-5	0.0	0.0	0.5421078799860312	0.0
2	7.398770028470468E-5	0.0	0.0	0.7941099871557352	0.0
3	7.398770028470468E-5	0.0	0.0	1.1162524341953393	0.0
4	7.398770028470468E-5	0.0	0.0	2.4247249137303415	0.0
5	7.398770028470468E-5	0.0	0.0	2.634997957939472	0.0
6	7.398770028470468E-5	0.0	0.0	3.816063617584213	0.0
7	7.398770028470468E-5	0.0	0.0	4.7292198144980375	0.0
8	7.398770028470468E-5	0.0	0.0	5.6356431306859545	0.0
9	7.398770028470468E-5	0.0	0.0	6.809827934204218	0.0
10	7.398770028470468E-5	0.0	0.0	8.515170438066376	0.0
11	7.398770028470468E-5	0.0	0.0	10.007280389708015	0.0
12	1.4797540056940935E-4	0.0	0.0	10.67494539707719	0.0
13	1.4797540056940935E-4	0.0	0.0	10.963719391288393	0.0
14	1.4797540056940935E-4	0.0	0.0	11.236586029938383	7.398770028470468E-5
15	1.4797540056940935E-4	0.0	0.0	11.477342006664813	0.002737544910534073
16	1.4797540056940935E-4	0.0	0.0	11.975353217281159	0.0028115326108187773
17	1.4797540056940935E-4	0.0	0.0	12.705167892889486	0.0028115326108187773
18	1.4797540056940935E-4	0.0	0.0	13.537751484193267	0.0028115326108187773
19	1.4797540056940935E-4	0.0	0.0	13.944979786560282	0.0028115326108187773
20	1.4797540056940935E-4	0.0	0.0	14.367005628984238	0.0028115326108187773
21	1.4797540056940935E-4	0.0	0.0	14.828836854161365	0.0028115326108187773
22	1.4797540056940935E-4	0.0	0.0	15.358810751300704	0.0028115326108187773
23	1.4797540056940935E-4	0.0	0.0	15.891078267148869	0.0028115326108187773
24	1.4797540056940935E-4	0.0	0.0	16.31428791277738	0.0028115326108187773
25	1.4797540056940935E-4	0.0	0.0	16.698062114154144	0.0028115326108187773
26	1.4797540056940935E-4	0.0	0.0	17.02923106062848	0.0028115326108187773
27	1.4797540056940935E-4	0.0	0.0	17.398577660449728	0.0028115326108187773
28	1.4797540056940935E-4	0.0	0.0	17.753718621816308	0.0028115326108187773
29	1.4797540056940935E-4	0.0	0.0	18.142080060610724	0.0028115326108187773
30	1.4797540056940935E-4	0.0	0.0	18.679304752377966	0.0028115326108187773
31	1.4797540056940935E-4	0.0	0.0	19.039772828165045	0.0028115326108187773
32	1.4797540056940935E-4	0.0	0.0	19.411339058994834	0.0028115326108187773
33	1.4797540056940935E-4	0.0	0.0	19.77565449519672	0.0028115326108187773
34	1.4797540056940935E-4	0.0	0.0	20.128871776355897	0.0028115326108187773
35	1.4797540056940935E-4	0.0	0.0	20.502805613594795	0.0028115326108187773
36	1.4797540056940935E-4	0.0	0.0	20.86519736958928	0.0028115326108187773
37	1.4797540056940935E-4	0.0	0.0	21.236097711116503	0.0028115326108187773
38	1.4797540056940935E-4	0.0	0.0	21.61062344995768	0.0028115326108187773
39	1.4797540056940935E-4	0.0	0.0	22.009343166791954	0.0028115326108187773
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACGT	35	1.2121382E-7	45.000004	20
CATAGCG	50	1.0822987E-9	40.5	1
CTAACGG	345	0.0	40.434784	2
TCGGACG	45	1.9286745E-8	40.0	1
CACTACG	45	1.9286745E-8	40.0	1
CGTTAGG	170	0.0	39.705883	2
TAACGGG	895	0.0	38.463688	3
ATAGGGA	905	0.0	38.287296	4
GTAGGGT	335	0.0	38.283585	4
CAGGGAA	1765	0.0	36.968838	5
ACGGGTC	335	0.0	36.9403	5
CGGGTCA	195	0.0	36.923077	6
CCAGGGA	1550	0.0	36.870968	4
ATGGGAC	635	0.0	36.850395	5
GATAGGG	795	0.0	36.509434	3
CATACGG	260	0.0	36.346157	2
AACGGGA	1135	0.0	36.277534	4
CTAGGGC	860	0.0	36.104652	4
AACGGGC	755	0.0	36.059605	4
GCGAGAC	100	0.0	36.0	21
>>END_MODULE