FastQCFastQC Report
Sat 18 Jun 2016
SRR3553141_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553141_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371897
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCT68290.49777789440460907No Hit
AATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCT27880.20322225356568316No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCC23970.17472157166317878No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGT23750.17311795273260308No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC14080.10263161155684428No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13800.10059064200883885No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACTC302.1658107E-644.99999635
TACCACG1150.039.1304361
CGTTAGG1850.038.918922
TAACGGG8500.038.382353
CCTCGCG659.094947E-1238.076921
CTAACGG3300.037.52
ACGCTAG900.037.51
ACGTCGA552.750312E-936.8181829
TGACGGG5950.036.680673
TTACGGG4500.036.53
GTAGGGT2850.036.315794
CTAGGGA10900.036.1238564
ATTAGCG504.8801667E-836.01
CCTACCG1000.036.01
TACGCGA250.002107250736.029
ACCGGGT3250.035.9999964
ACGGGTC3200.035.8593755
TACGGGA8650.035.6358384
ACGGGTG4300.035.5813945
ACGCACG950.035.5263141
Produced by FastQC (version 0.11.5)