##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553141_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1371897
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.78000972376206	31.0	31.0	34.0	30.0	34.0
2	31.986553655267123	33.0	31.0	34.0	30.0	34.0
3	32.13082031668558	34.0	31.0	34.0	30.0	34.0
4	35.794775409524185	37.0	35.0	37.0	35.0	37.0
5	35.581238970564115	37.0	35.0	37.0	33.0	37.0
6	35.60463139725504	37.0	35.0	37.0	33.0	37.0
7	35.76443056585152	37.0	35.0	37.0	35.0	37.0
8	35.83494533481741	37.0	35.0	37.0	35.0	37.0
9	37.52659711334014	39.0	37.0	39.0	35.0	39.0
10	37.02575922244892	39.0	37.0	39.0	33.0	39.0
11	37.00319338842493	39.0	37.0	39.0	33.0	39.0
12	36.92894583193928	39.0	37.0	39.0	33.0	39.0
13	36.9121996767979	39.0	37.0	39.0	33.0	39.0
14	38.077739072248136	40.0	37.0	41.0	33.0	41.0
15	38.06364544860146	40.0	37.0	41.0	33.0	41.0
16	37.99394633853708	40.0	37.0	41.0	33.0	41.0
17	37.97430346447292	40.0	37.0	41.0	33.0	41.0
18	37.92147369664049	40.0	37.0	41.0	33.0	41.0
19	37.9969042865463	40.0	37.0	41.0	33.0	41.0
20	37.91296576929609	40.0	37.0	41.0	33.0	41.0
21	37.8969521764389	40.0	37.0	41.0	33.0	41.0
22	37.878054256259766	40.0	37.0	41.0	33.0	41.0
23	37.761289659500676	40.0	37.0	41.0	33.0	41.0
24	37.617970591086646	39.0	36.0	41.0	32.0	41.0
25	37.66516218054271	39.0	36.0	41.0	33.0	41.0
26	37.57204950517422	39.0	36.0	41.0	32.0	41.0
27	37.539206660558335	39.0	36.0	41.0	32.0	41.0
28	37.49324985767882	39.0	36.0	41.0	32.0	41.0
29	37.32183319884802	39.0	36.0	41.0	32.0	41.0
30	37.390122582088885	39.0	36.0	41.0	32.0	41.0
31	37.32834097603537	39.0	36.0	41.0	32.0	41.0
32	37.280854903830246	39.0	36.0	41.0	32.0	41.0
33	37.25546524265306	39.0	36.0	41.0	32.0	41.0
34	37.22281191663806	39.0	36.0	41.0	32.0	41.0
35	37.144798042418635	39.0	36.0	41.0	31.0	41.0
36	37.081347943759624	39.0	35.0	41.0	31.0	41.0
37	37.03587951573624	39.0	35.0	41.0	31.0	41.0
38	36.98505645832012	39.0	35.0	41.0	31.0	41.0
39	36.9051022051947	39.0	35.0	41.0	31.0	41.0
40	36.82017381771372	39.0	35.0	41.0	31.0	41.0
41	36.709984058569994	39.0	35.0	40.0	31.0	41.0
42	36.59805145721581	39.0	35.0	40.0	30.0	41.0
43	36.49199611924219	39.0	35.0	40.0	30.0	41.0
44	36.438109420750976	39.0	35.0	40.0	30.0	41.0
45	36.287312385696595	38.0	35.0	40.0	30.0	41.0
46	36.17044501154241	38.0	35.0	40.0	30.0	41.0
47	36.03787310563402	38.0	35.0	40.0	30.0	41.0
48	35.9644033043297	38.0	35.0	40.0	29.0	41.0
49	35.80051053395408	38.0	35.0	40.0	29.0	41.0
50	35.70189963240681	38.0	34.0	40.0	28.0	41.0
51	34.428204887101586	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	6.0
10	6.0
11	7.0
12	19.0
13	13.0
14	35.0
15	58.0
16	90.0
17	190.0
18	357.0
19	721.0
20	1234.0
21	1868.0
22	2689.0
23	3707.0
24	5000.0
25	6409.0
26	8377.0
27	10675.0
28	13844.0
29	18144.0
30	23698.0
31	31096.0
32	40974.0
33	55055.0
34	84328.0
35	104677.0
36	125919.0
37	185615.0
38	290537.0
39	356449.0
40	95.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.670281369519724	31.5703001027045	22.47063737292231	26.288781154853464
2	22.204436630446747	29.554186648123	21.546442626523714	26.694934094906543
3	21.428503743356828	28.746035598882425	22.34344123501983	27.482019422740922
4	20.5426500677529	30.213784270976614	20.571588100272834	28.671977560997654
5	18.253702719664812	33.34929663086952	19.524424938606906	28.87257571085876
6	20.33315912200406	35.82047340288666	20.59790202908819	23.248465446021093
7	85.34510972762533	4.497495074338671	5.456823653670793	4.700571544365211
8	87.63033959546526	3.256585589151372	4.163213419083211	4.94986139630016
9	79.88486016078467	6.386922633404694	8.313597886721817	5.414619319088824
10	36.10766697499885	27.99371964513371	14.556267708144272	21.342345671723166
11	25.399064215462243	27.131993145257987	26.97206860281785	20.49687403646192
12	23.540907225542444	21.323320919864976	35.169112549994644	19.96665930459794
13	25.491418087509487	24.016307346688563	28.632397330120263	21.859877235681687
14	21.413706714133788	27.434202421901936	28.700405351130588	22.451685512833688
15	19.610072767853566	31.802095929942265	28.13352605917208	20.454305243032096
16	21.58871985287525	28.82104122977162	26.946993834085216	22.643245083267914
17	19.773641898772283	29.70995635969756	28.46416312594896	22.0522386155812
18	20.288986709643652	29.46722676702406	28.731821703815957	21.511964819516333
19	19.97824909595983	33.178365431224066	28.23200283986334	18.61138263295277
20	20.79573029170557	30.97389964406949	28.227119091302043	20.003250972922896
21	20.60497253073664	30.183023944217386	28.467589039118824	20.74441448592715
22	19.57085699582403	29.109619745505675	28.939053004708082	22.380470253962216
23	20.015351006671782	29.13658970024718	27.778834708436566	23.069224584644473
24	18.518591410288092	30.305627900636857	28.32807419215874	22.847706496916313
25	20.266098693998163	29.10531913110095	27.398631238350983	23.2299509365499
26	20.777726024621384	30.098688166823017	27.09569304401132	22.027892764544276
27	18.445699640716466	28.603167730522046	28.891746246256094	24.059386382505394
28	19.472161539824054	28.90362760469627	29.195850708908903	22.428360146570768
29	19.727792975711733	31.48705770185371	26.88124545793161	21.903903864502947
30	20.13285253922124	29.07798471751159	28.015732959544337	22.773429783722833
31	19.369821495345498	30.505642916341387	26.384342264761855	23.74019332355126
32	19.556715992527135	28.990077243408212	28.282881295024335	23.170325469040314
33	19.36996727888464	28.15488334765657	30.325891812577765	22.14925756088103
34	17.47048065561773	27.459714541252005	28.80427612277015	26.265528680360113
35	19.123447314193413	27.59456431495951	30.513588119224693	22.76840025162239
36	18.486956382294007	29.101455867313653	30.791961787218717	21.61962596317362
37	19.26310794469264	29.49805998555285	28.705216207922312	22.533615861832192
38	20.004635916544757	27.56154434334356	30.42181738133402	22.012002358777664
39	18.927076886967463	27.657469912099813	29.24505265336975	24.170400547562974
40	20.45860585743682	27.359196791012735	28.194900929151384	23.98729642239906
41	19.322150278045655	27.231563302492827	29.32960710607283	24.116679313388687
42	20.768833228733644	28.171211104040612	28.032716741854525	23.02723892537122
43	18.599355490973448	27.414594535887172	29.9895691877743	23.996480785365083
44	20.063896925206485	26.046416021027817	29.736634747360775	24.153052306404927
45	20.8814510127218	25.930955458026368	30.446965041836233	22.740628487415602
46	18.243424980155215	27.340682281541547	31.288354738001466	23.127538000301772
47	19.277176056219965	27.389228200076243	29.132799328229453	24.20079641547434
48	19.67319704030259	25.9701712300559	31.378813424039855	22.97781830560166
49	20.424273833968584	25.944148868318834	29.667387566267728	23.964189731444854
50	19.575449177307043	25.578013509760574	29.032791820377184	25.813745492555203
51	19.57938533286391	24.81148366094539	31.848090636541954	23.761040369648743
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1687.0
1	1366.0
2	1045.0
3	761.5
4	478.0
5	583.0
6	688.0
7	704.5
8	721.0
9	816.0
10	911.0
11	1184.0
12	1457.0
13	1596.0
14	1735.0
15	2055.5
16	2376.0
17	3076.0
18	3776.0
19	4186.5
20	4597.0
21	5892.5
22	7188.0
23	9005.5
24	10823.0
25	14232.0
26	21089.5
27	24538.0
28	28613.5
29	32689.0
30	37811.0
31	42933.0
32	46483.0
33	50033.0
34	53394.0
35	56755.0
36	60007.5
37	63260.0
38	65602.0
39	67944.0
40	68723.5
41	69503.0
42	72107.0
43	74711.0
44	82112.5
45	89514.0
46	99539.0
47	109564.0
48	115581.5
49	121599.0
50	115032.5
51	108466.0
52	96815.5
53	85165.0
54	75377.0
55	65589.0
56	59426.0
57	53263.0
58	49798.5
59	46334.0
60	43239.0
61	40144.0
62	36034.0
63	31924.0
64	28430.0
65	24936.0
66	21654.0
67	18372.0
68	15806.0
69	13240.0
70	11328.5
71	9417.0
72	7718.5
73	6020.0
74	4876.0
75	3262.5
76	2793.0
77	2164.5
78	1536.0
79	1346.5
80	1157.0
81	1052.0
82	947.0
83	670.0
84	393.0
85	283.5
86	174.0
87	112.5
88	51.0
89	52.0
90	53.0
91	34.0
92	15.0
93	11.0
94	7.0
95	4.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1371897.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.227466712339584
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.3607603513319	20.990158875214824
2	10.031153109779163	5.66308080985346
3	3.7895059262728616	3.209044571702423
4	2.053993649968806	2.319161495274054
5	1.2718210167472008	1.7950142707142749
6	0.8796200951757436	1.4897668173646965
7	0.6714731746395485	1.3267790679756788
8	0.5571388017922585	1.258129358539499
9	0.46434120177257276	1.179645823458275
>10	4.663462152836749	29.039505225540207
>50	0.9738666190674773	19.171018184817193
>100	0.27660123124476105	10.370609822745326
>500	0.00443605747106112	0.8244544193234858
>1k	0.0015656673427274542	0.8606205159810245
>5k	2.6094455712124236E-4	0.5030107414955851
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCT	6829	0.49777789440460907	No Hit
AATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCT	2788	0.20322225356568316	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCC	2397	0.17472157166317878	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGT	2375	0.17311795273260308	No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	1408	0.10263161155684428	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1380	0.10059064200883885	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.289176957162236E-5	0.0	0.0	0.5724190664459504	0.0
2	7.289176957162236E-5	0.0	0.0	0.8356312463690787	0.0
3	7.289176957162236E-5	0.0	0.0	1.188573194634874	0.0
4	7.289176957162236E-5	0.0	0.0	2.573006574108698	0.0
5	7.289176957162236E-5	0.0	0.0	2.7942330947585714	0.0
6	7.289176957162236E-5	0.0	0.0	4.063497478309231	0.0
7	7.289176957162236E-5	0.0	0.0	5.010726023892464	0.0
8	7.289176957162236E-5	0.0	0.0	5.95766300239741	0.0
9	7.289176957162236E-5	0.0	0.0	7.2014881583675745	0.0
10	7.289176957162236E-5	0.0	0.0	8.984639517398172	0.0
11	7.289176957162236E-5	0.0	0.0	10.541826390756741	0.0
12	7.289176957162236E-5	0.0	0.0	11.237869898396163	0.0
13	7.289176957162236E-5	0.0	0.0	11.536215911252812	0.0
14	7.289176957162236E-5	0.0	0.0	11.823555266904147	0.0
15	7.289176957162236E-5	0.0	0.0	12.075031871926246	0.004154830865582475
16	7.289176957162236E-5	0.0	0.0	12.597592967985205	0.004154830865582475
17	7.289176957162236E-5	0.0	0.0	13.3314673040323	0.004154830865582475
18	7.289176957162236E-5	0.0	0.0	14.193631154525448	0.004154830865582475
19	7.289176957162236E-5	0.0	0.0	14.621214274832585	0.004154830865582475
20	7.289176957162236E-5	0.0	0.0	15.061626346584328	0.004154830865582475
21	7.289176957162236E-5	0.0	0.0	15.534256580486728	0.004154830865582475
22	7.289176957162236E-5	0.0	0.0	16.08174666173918	0.004154830865582475
23	7.289176957162236E-5	0.0	0.0	16.621510215417047	0.004154830865582475
24	7.289176957162236E-5	0.0	0.0	17.06104758593393	0.004154830865582475
25	7.289176957162236E-5	0.0	0.0	17.446645046967813	0.004154830865582475
26	7.289176957162236E-5	0.0	0.0	17.780562243375414	0.004154830865582475
27	7.289176957162236E-5	0.0	0.0	18.162806683009002	0.004154830865582475
28	7.289176957162236E-5	0.0	0.0	18.537834837454998	0.004154830865582475
29	7.289176957162236E-5	0.0	0.0	18.931741960220045	0.004154830865582475
30	7.289176957162236E-5	0.0	0.0	19.48054409332479	0.004154830865582475
31	1.4578353914324472E-4	0.0	0.0	19.854187304148926	0.004154830865582475
32	1.4578353914324472E-4	0.0	0.0	20.232131129377787	0.004154830865582475
33	1.4578353914324472E-4	0.0	0.0	20.602567102340775	0.004154830865582475
34	1.4578353914324472E-4	0.0	0.0	20.96097593332444	0.004154830865582475
35	1.4578353914324472E-4	0.0	0.0	21.343876398884174	0.004154830865582475
36	1.4578353914324472E-4	0.0	0.0	21.71300031999487	0.004154830865582475
37	2.1867530871486708E-4	0.0	0.0	22.083509184727426	0.004154830865582475
38	2.1867530871486708E-4	0.0	0.0	22.461817468804146	0.004154830865582475
39	2.1867530871486708E-4	0.0	0.0	22.858713154121627	0.004154830865582475
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGACTC	30	2.1658107E-6	44.999996	35
TACCACG	115	0.0	39.130436	1
CGTTAGG	185	0.0	38.91892	2
TAACGGG	850	0.0	38.38235	3
CCTCGCG	65	9.094947E-12	38.07692	1
CTAACGG	330	0.0	37.5	2
ACGCTAG	90	0.0	37.5	1
ACGTCGA	55	2.750312E-9	36.81818	29
TGACGGG	595	0.0	36.68067	3
TTACGGG	450	0.0	36.5	3
GTAGGGT	285	0.0	36.31579	4
CTAGGGA	1090	0.0	36.123856	4
ATTAGCG	50	4.8801667E-8	36.0	1
CCTACCG	100	0.0	36.0	1
TACGCGA	25	0.0021072507	36.0	29
ACCGGGT	325	0.0	35.999996	4
ACGGGTC	320	0.0	35.859375	5
TACGGGA	865	0.0	35.635838	4
ACGGGTG	430	0.0	35.581394	5
ACGCACG	95	0.0	35.526314	1
>>END_MODULE