FastQCFastQC Report
Sat 18 Jun 2016
SRR3553140_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553140_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1351752
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGCT72440.5358971172226858TruSeq Adapter, Index 13 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCT24980.18479721132278704No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGT16770.12406121832998954No Hit
GGCCCTGGGAATGATACCTGTCTCTTATACACATCTGACGCGAGTTCACTC16470.12184187632050851No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCC16000.11836490717232154No Hit
CCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC15840.11718125810059833TruSeq Adapter, Index 19 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCCG501.0822987E-940.51
CAGGGTA5150.040.1941765
TTAGCCG403.4588265E-739.3750041
CGGTCTA601.5643309E-1037.50000431
ATAGGGA10100.037.202974
TATCCGG2000.037.1252
TAGCACG1300.036.3461531
GCCCTGG17100.035.9210552
ACGAGGG6900.035.8695643
ACGGGTA5150.035.825245
GGGAATG36450.035.7407387
TAGGGTC2650.035.6603785
CACTAGG2850.035.5263182
CATGCGG3000.035.252
ACGGGTC3450.035.2173925
TAAGGGA8650.035.1156044
GGCCGAT1350.035.08
TCTAGCG1350.035.01
AGGGAGT10600.034.811326
TGCGAAG1750.034.7142871
Produced by FastQC (version 0.11.5)