##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553140_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1351752
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.737795098509196	31.0	31.0	34.0	30.0	34.0
2	31.94426788345791	33.0	31.0	34.0	30.0	34.0
3	32.122577958086985	34.0	31.0	34.0	30.0	34.0
4	35.78567814214442	37.0	35.0	37.0	35.0	37.0
5	35.56204984346241	37.0	35.0	37.0	33.0	37.0
6	34.878326793672215	37.0	35.0	37.0	33.0	37.0
7	35.390714420988466	37.0	35.0	37.0	33.0	37.0
8	35.81487802496316	37.0	35.0	37.0	35.0	37.0
9	37.44905796329504	39.0	37.0	39.0	35.0	39.0
10	36.98394084121939	39.0	37.0	39.0	33.0	39.0
11	36.98569412140689	39.0	37.0	39.0	33.0	39.0
12	36.92330249927502	39.0	37.0	39.0	33.0	39.0
13	36.931633169397934	39.0	37.0	39.0	33.0	39.0
14	38.10781341547858	40.0	37.0	41.0	33.0	41.0
15	38.107562629831506	40.0	37.0	41.0	33.0	41.0
16	37.995960057762076	40.0	37.0	41.0	33.0	41.0
17	37.99295580846191	40.0	37.0	41.0	33.0	41.0
18	37.99323322621309	40.0	37.0	41.0	33.0	41.0
19	38.03185939432677	40.0	37.0	41.0	33.0	41.0
20	37.938649249270576	40.0	37.0	41.0	33.0	41.0
21	37.948435807751714	40.0	37.0	41.0	33.0	41.0
22	37.923432700672905	40.0	37.0	41.0	33.0	41.0
23	37.82671451568039	40.0	37.0	41.0	33.0	41.0
24	37.704564150820566	40.0	37.0	41.0	33.0	41.0
25	37.72609472743521	40.0	37.0	41.0	33.0	41.0
26	37.67477318324663	40.0	37.0	41.0	33.0	41.0
27	37.626765856458874	40.0	37.0	41.0	33.0	41.0
28	37.523849789014555	40.0	36.0	41.0	32.0	41.0
29	37.27065171717889	39.0	36.0	41.0	31.0	41.0
30	37.37851543774302	39.0	36.0	41.0	32.0	41.0
31	37.237916422539044	39.0	36.0	41.0	31.0	41.0
32	37.29848078641644	39.0	36.0	41.0	32.0	41.0
33	37.03848413022507	39.0	36.0	41.0	31.0	41.0
34	37.03872086003942	39.0	36.0	41.0	31.0	41.0
35	37.003176618196235	39.0	35.0	41.0	31.0	41.0
36	36.92870437772609	39.0	35.0	41.0	31.0	41.0
37	36.8670562351674	39.0	35.0	41.0	31.0	41.0
38	36.73320106054957	39.0	35.0	41.0	30.0	41.0
39	36.75060218146524	39.0	35.0	41.0	31.0	41.0
40	36.722764234859646	39.0	35.0	40.0	31.0	41.0
41	36.70620498434624	39.0	35.0	40.0	31.0	41.0
42	36.66045176925945	39.0	35.0	40.0	31.0	41.0
43	36.57440935911321	39.0	35.0	40.0	31.0	41.0
44	36.47871946925176	39.0	35.0	40.0	30.0	41.0
45	36.257395587356264	38.0	35.0	40.0	30.0	41.0
46	36.12584852842829	38.0	35.0	40.0	29.0	41.0
47	36.013372275387795	38.0	35.0	40.0	29.0	41.0
48	35.91745971154472	38.0	35.0	40.0	29.0	41.0
49	35.84149903236688	38.0	34.0	40.0	29.0	41.0
50	35.75965783664459	38.0	34.0	40.0	29.0	41.0
51	34.56716912569761	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	9.0
11	10.0
12	11.0
13	16.0
14	22.0
15	50.0
16	118.0
17	188.0
18	380.0
19	603.0
20	1289.0
21	2111.0
22	2810.0
23	3708.0
24	4919.0
25	6215.0
26	8148.0
27	10502.0
28	13588.0
29	17989.0
30	23354.0
31	30964.0
32	40780.0
33	55447.0
34	82450.0
35	104354.0
36	129320.0
37	190208.0
38	285628.0
39	336470.0
40	86.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.166234634755487	31.027880853884444	21.16438518308092	26.64149932827915
2	22.710378826885407	28.17284531482106	21.881602542478205	27.235173315815324
3	21.157431244784547	28.627662470630703	22.28322946812729	27.931676816457458
4	20.149480082145246	29.416490598867245	20.92003562783706	29.513993691150446
5	18.411587332587633	32.20294846983766	19.31315803490581	30.072306162668895
6	20.15754369144636	35.62169687930922	21.023382987411892	23.197376441832525
7	85.09216187584705	4.208094384176979	5.854328308743024	4.845415431232948
8	87.67096331279703	3.0207464091046283	4.424332273967415	4.883958004130935
9	79.37743017950038	6.523533902668537	8.5224952506081	5.576540667222981
10	34.46098100835064	28.666574933863608	15.650503938592287	21.221940119193462
11	23.997153323982506	26.991193650906382	28.180465055720276	20.831187969390836
12	22.164568648687037	21.34955228473862	36.25480117654718	20.231077890027166
13	25.13508395031041	23.616610147423494	29.692132876444795	21.556173025821305
14	20.38399055448041	27.912664453242904	29.824997484745726	21.878347507530968
15	19.74696541969237	30.96618314602087	29.27334303925572	20.01350839503104
16	20.035479880924903	29.316990098775513	28.04493723700797	22.60259278329161
17	18.830895016245584	30.12349898502092	29.158529079298567	21.887076919434925
18	20.30520391314383	29.318173747847236	28.501603844492184	21.875018494516745
19	19.95728506412419	32.20731317578964	28.73811172463588	19.09729003545029
20	20.610215483313507	30.089395096141896	28.8269593830821	20.473430037462492
21	20.794716782368365	30.814971977108225	28.100864655646895	20.289446584876515
22	18.672212062567688	30.108481437423436	28.673380916026016	22.54592558398286
23	19.236590735578716	29.591670661482283	27.90460084394179	23.26713775899721
24	17.37996318851387	30.003728494575927	28.72634921198563	23.88995910492457
25	20.007738105806393	30.36585113245625	27.424409211157077	22.202001550580285
26	20.55443602080855	30.57402541294557	27.366854275044535	21.504684291201347
27	19.26374068616137	29.101122099319994	27.89890453278412	23.73623268173452
28	21.060445998970227	29.35605051814238	29.161118311642966	20.42238517124443
29	19.82197917961283	31.756860725931972	27.173993454420632	21.24716664003456
30	22.05042049133273	29.27511851286331	26.78279743621611	21.891663559587855
31	19.933390148488776	31.86901147547775	26.654223555800176	21.543374820233296
32	21.724103237872036	29.25876936006013	27.065985476625894	21.951141925441945
33	20.947259556486692	28.37436156928194	28.263838337209783	22.414540537021583
34	19.72181287691825	29.239239150376694	29.28340405636537	21.755543916339683
35	20.713858755156274	27.792450094396017	28.970032964626647	22.52365818582107
36	20.213323153951315	30.680331895199714	29.15150116293521	19.95484378791376
37	19.91400789493931	29.563928886363772	28.306967550260698	22.21509566843622
38	19.704575987311284	28.528753795074834	30.72405293278649	21.042617284827394
39	18.91493410033793	27.942107723902016	29.38830495534684	23.754653220413214
40	20.029117767164394	29.675783723641615	27.230586675662398	23.064511833531594
41	18.00345033704407	29.10297155099456	29.395924696246055	23.49765341571531
42	20.779181388301996	29.49905012161994	27.134637122785836	22.587131367292226
43	19.18850499203996	27.46990572235144	29.670309346684892	23.67127993892371
44	21.334608715208113	26.1542058010641	28.596813616698917	23.914371867028862
45	21.653454183903555	26.492137611041077	30.10892530582533	21.745482899230037
46	19.174153247045318	27.624371926211317	31.218152442163948	21.98332238457942
47	19.682530523350437	28.415123484189408	28.19392906391113	23.708416928549024
48	19.56705076079044	27.304416786511137	30.937183743763647	22.191348708934775
49	19.918298622824302	26.19075096615355	29.63583556747096	24.255114843551183
50	19.12214666595648	25.731347170190983	29.05666128106339	26.089844882789148
51	19.366866111535252	25.200406583456136	31.14210298930573	24.29062431570288
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1657.0
1	1348.0
2	1039.0
3	775.5
4	512.0
5	551.0
6	590.0
7	649.0
8	708.0
9	825.0
10	942.0
11	1166.5
12	1391.0
13	1479.0
14	1567.0
15	1784.5
16	2002.0
17	2729.0
18	3456.0
19	4221.5
20	4987.0
21	6183.5
22	7380.0
23	9316.5
24	11253.0
25	14288.5
26	20954.0
27	24584.0
28	29538.5
29	34493.0
30	37589.5
31	40686.0
32	45379.0
33	50072.0
34	53116.0
35	56160.0
36	60556.5
37	64953.0
38	65671.5
39	66390.0
40	69125.0
41	71860.0
42	71634.5
43	71409.0
44	80500.0
45	89591.0
46	97826.0
47	106061.0
48	115533.0
49	125005.0
50	118845.5
51	112686.0
52	98849.5
53	85013.0
54	74268.5
55	63524.0
56	56525.0
57	49526.0
58	45749.5
59	41973.0
60	39551.5
61	37130.0
62	34642.0
63	32154.0
64	27127.5
65	22101.0
66	19276.0
67	16451.0
68	13989.5
69	11528.0
70	9738.0
71	7948.0
72	6836.5
73	5725.0
74	4945.0
75	3338.0
76	2511.0
77	2082.5
78	1654.0
79	1169.5
80	685.0
81	588.0
82	491.0
83	388.5
84	286.0
85	168.5
86	51.0
87	48.0
88	45.0
89	30.5
90	16.0
91	9.5
92	3.0
93	6.0
94	9.0
95	7.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1351752.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.30141664352293
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.96969199353121	21.674167640955208
2	10.182065071547477	5.966978619057495
3	3.8869704717827522	3.4168122382433204
4	1.982249545717614	2.3233087932202348
5	1.3135292305168946	1.92441336284108
6	0.93134306458339	1.637380270404804
7	0.6984146530458902	1.43251771171874
8	0.5754260257583803	1.3488638182618287
9	0.48801903607375	1.2869684195370659
>10	4.896527377794763	31.241307565679723
>50	0.8914027038306251	18.266070812810593
>100	0.1776567958174204	7.199437193672
>500	0.00464125153839418	0.8789070477469121
>1k	0.0018049311538199588	0.8555611973425628
>5k	2.578473076885655E-4	0.5473053085084356
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGCT	7244	0.5358971172226858	TruSeq Adapter, Index 13 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCT	2498	0.18479721132278704	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGT	1677	0.12406121832998954	No Hit
GGCCCTGGGAATGATACCTGTCTCTTATACACATCTGACGCGAGTTCACTC	1647	0.12184187632050851	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCC	1600	0.11836490717232154	No Hit
CCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	1584	0.11718125810059833	TruSeq Adapter, Index 19 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4795613396540193E-4	0.0	0.0	0.6052885440524594	0.0
2	1.4795613396540193E-4	0.0	0.0	0.8975019086341282	0.0
3	1.4795613396540193E-4	0.0	0.0	1.2692416952222005	0.0
4	1.4795613396540193E-4	0.0	0.0	2.750948398818718	0.0
5	1.4795613396540193E-4	0.0	0.0	3.0038794098325727	0.0
6	1.4795613396540193E-4	0.0	0.0	4.3499843166498	0.0
7	1.4795613396540193E-4	0.0	0.0	5.348984133184193	0.0
8	1.4795613396540193E-4	0.0	0.0	6.261947457817707	0.0
9	1.4795613396540193E-4	0.0	0.0	7.4887257425918365	0.0
10	1.4795613396540193E-4	0.0	0.0	9.36525338967503	0.0
11	1.4795613396540193E-4	0.0	0.0	11.004163485609787	0.0
12	1.4795613396540193E-4	0.0	0.0	11.801795003817269	0.0
13	1.4795613396540193E-4	0.0	0.0	12.153782646520959	0.0
14	1.4795613396540193E-4	0.0	0.0	12.4687812557333	0.0
15	1.4795613396540193E-4	0.0	0.0	12.729627919914304	0.0
16	1.4795613396540193E-4	0.0	0.0	13.351709485171837	0.0
17	1.4795613396540193E-4	0.0	0.0	14.161547384431463	0.0
18	1.4795613396540193E-4	0.0	0.0	15.181778906189892	0.0
19	1.4795613396540193E-4	0.0	0.0	15.634968544525918	0.0
20	1.4795613396540193E-4	0.0	0.0	16.151779320467067	0.0
21	1.4795613396540193E-4	0.0	0.0	16.661414223910896	0.0
22	1.4795613396540193E-4	0.0	0.0	17.24103237872036	0.0
23	1.4795613396540193E-4	0.0	0.0	17.830711550639467	0.0
24	1.4795613396540193E-4	0.0	0.0	18.297661109434276	0.0
25	1.4795613396540193E-4	0.0	0.0	18.729397108345317	0.0
26	1.4795613396540193E-4	0.0	0.0	19.088708579680297	0.0
27	1.4795613396540193E-4	0.0	0.0	19.537829424332273	0.0
28	1.4795613396540193E-4	0.0	0.0	19.94589244180885	0.0
29	1.4795613396540193E-4	0.0	0.0	20.374891252241536	0.0
30	1.4795613396540193E-4	0.0	0.0	21.000449786647255	0.0
31	1.4795613396540193E-4	0.0	0.0	21.39586255466979	0.0
32	1.4795613396540193E-4	0.0	0.0	21.796675721582066	0.0
33	1.4795613396540193E-4	0.0	0.0	22.176775029739183	0.0
34	1.4795613396540193E-4	0.0	0.0	22.543632263906396	0.0
35	2.219342009481029E-4	0.0	0.0	22.944815321153584	0.0
36	2.219342009481029E-4	0.0	0.0	23.31877444975114	0.0
37	2.219342009481029E-4	0.0	0.0	23.689330587267488	0.0
38	2.219342009481029E-4	0.0	0.0	24.081414342275803	0.0
39	2.219342009481029E-4	0.0	0.0	24.487331995809882	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACCCG	50	1.0822987E-9	40.5	1
CAGGGTA	515	0.0	40.194176	5
TTAGCCG	40	3.4588265E-7	39.375004	1
CGGTCTA	60	1.5643309E-10	37.500004	31
ATAGGGA	1010	0.0	37.20297	4
TATCCGG	200	0.0	37.125	2
TAGCACG	130	0.0	36.346153	1
GCCCTGG	1710	0.0	35.921055	2
ACGAGGG	690	0.0	35.869564	3
ACGGGTA	515	0.0	35.82524	5
GGGAATG	3645	0.0	35.740738	7
TAGGGTC	265	0.0	35.660378	5
CACTAGG	285	0.0	35.526318	2
CATGCGG	300	0.0	35.25	2
ACGGGTC	345	0.0	35.217392	5
TAAGGGA	865	0.0	35.115604	4
GGCCGAT	135	0.0	35.0	8
TCTAGCG	135	0.0	35.0	1
AGGGAGT	1060	0.0	34.81132	6
TGCGAAG	175	0.0	34.714287	1
>>END_MODULE