FastQCFastQC Report
Sat 18 Jun 2016
SRR3553139_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553139_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371744
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGCT76750.5595067301187393TruSeq Adapter, Index 13 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCT27780.2025159213380922No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGT19360.1411342057993328No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCC18400.13413581542911795No Hit
GGCCCTGGGAATGATACCTGTCTCTTATACACATCTGACGCGAGTTCACTC18370.13391711573004877No Hit
CCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC17140.12495042806821097TruSeq Adapter, Index 19 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAG451.9288564E-840.01
CCGATAG800.039.3751
CGACGTC356.2491745E-638.57142618
CCATGCG659.094947E-1238.076921
TAAGGGA8850.036.8644074
TTACGGG8550.036.578953
CACTAGG3100.036.2903252
TGAGCGG2550.036.176472
CGACAGG3000.036.0000042
GCGACGT250.002107250735.99999617
CGCGCAT250.002107250735.99999623
ACGGCAC250.002107250735.99999624
ACTACGT250.002107250735.99999616
TAGTGCG250.002107250735.9999961
ACGACTC250.002107250735.99999633
CGAGGGA7350.035.5102044
TAAGGGC8500.035.470594
GTAAGGG8700.035.4310343
CGACACG702.1827873E-1135.3571431
CATGCGG2550.035.2941172
Produced by FastQC (version 0.11.5)