##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553139_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1371744
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75603611169431	31.0	31.0	34.0	30.0	34.0
2	31.9604087934775	33.0	31.0	34.0	30.0	34.0
3	32.11560393192899	34.0	31.0	34.0	30.0	34.0
4	35.77935387360907	37.0	35.0	37.0	35.0	37.0
5	35.574763950124805	37.0	35.0	37.0	33.0	37.0
6	35.60038243287377	37.0	35.0	37.0	33.0	37.0
7	35.77069701052091	37.0	35.0	37.0	35.0	37.0
8	35.830541267175214	37.0	35.0	37.0	35.0	37.0
9	37.4871251487158	39.0	37.0	39.0	35.0	39.0
10	37.01441158116966	39.0	37.0	39.0	33.0	39.0
11	37.00363478899853	39.0	37.0	39.0	33.0	39.0
12	36.9628042841813	39.0	37.0	39.0	33.0	39.0
13	36.96848318636714	39.0	37.0	39.0	33.0	39.0
14	38.13799951011267	40.0	37.0	41.0	33.0	41.0
15	38.13992115146849	40.0	37.0	41.0	33.0	41.0
16	38.034858545034645	40.0	37.0	41.0	33.0	41.0
17	38.00111391046726	40.0	37.0	41.0	33.0	41.0
18	37.964204691254345	40.0	37.0	41.0	33.0	41.0
19	38.025050592530384	40.0	37.0	41.0	33.0	41.0
20	37.94490808780647	40.0	37.0	41.0	33.0	41.0
21	37.93810579816642	40.0	37.0	41.0	33.0	41.0
22	37.90659991951851	40.0	37.0	41.0	33.0	41.0
23	37.78216270674412	40.0	37.0	41.0	33.0	41.0
24	37.67449101290037	39.0	37.0	41.0	32.0	41.0
25	37.69890300230947	40.0	37.0	41.0	33.0	41.0
26	37.63334776751347	40.0	37.0	41.0	33.0	41.0
27	37.61549385308046	40.0	37.0	41.0	33.0	41.0
28	37.502009121235446	40.0	36.0	41.0	32.0	41.0
29	37.29824223761868	39.0	36.0	41.0	32.0	41.0
30	37.38124241841043	39.0	36.0	41.0	32.0	41.0
31	37.205174580679774	39.0	36.0	41.0	31.0	41.0
32	37.262155329274265	39.0	36.0	41.0	32.0	41.0
33	37.144427823267314	39.0	36.0	41.0	31.0	41.0
34	37.09021362586605	39.0	36.0	41.0	31.0	41.0
35	37.02396438402501	39.0	35.0	41.0	31.0	41.0
36	36.90828172020435	39.0	35.0	41.0	31.0	41.0
37	36.85025048405534	39.0	35.0	41.0	31.0	41.0
38	36.814235746611615	39.0	35.0	41.0	31.0	41.0
39	36.785150873632396	39.0	35.0	41.0	31.0	41.0
40	36.74333694916836	39.0	35.0	40.0	31.0	41.0
41	36.66639475004082	39.0	35.0	40.0	31.0	41.0
42	36.61054322089253	39.0	35.0	40.0	30.0	41.0
43	36.5046750705671	39.0	35.0	40.0	30.0	41.0
44	36.43517668019689	39.0	35.0	40.0	30.0	41.0
45	36.22281708540369	38.0	35.0	40.0	30.0	41.0
46	36.104089392773	38.0	35.0	40.0	29.0	41.0
47	35.96922968133996	38.0	35.0	40.0	29.0	41.0
48	35.89358728742389	38.0	35.0	40.0	29.0	41.0
49	35.748105331607064	38.0	34.0	40.0	28.0	41.0
50	35.67771537546364	38.0	34.0	40.0	28.0	41.0
51	34.416531801852244	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	5.0
11	11.0
12	10.0
13	13.0
14	23.0
15	55.0
16	110.0
17	206.0
18	364.0
19	638.0
20	1206.0
21	1972.0
22	2916.0
23	3917.0
24	5124.0
25	6472.0
26	8291.0
27	10845.0
28	14000.0
29	18175.0
30	23819.0
31	31016.0
32	40889.0
33	55494.0
34	82851.0
35	104246.0
36	128726.0
37	190651.0
38	294504.0
39	345109.0
40	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.233262183031236	31.050837474047636	21.09621037161453	26.6196899713066
2	22.655539225978025	28.358862878204675	21.89162117712926	27.093976718688033
3	21.18755394592577	28.550079315090866	22.341413558214942	27.92095318076842
4	20.180806331210487	29.302697879487717	21.01252128677071	29.503974502531083
5	18.384990202253483	32.2367730422003	19.33123090022628	30.04700585531994
6	20.546034828656076	34.22198311055124	21.56393612802389	23.6680459327688
7	84.88107110364616	4.22433048732125	5.975823477266895	4.918774931765694
8	87.25090104276016	3.109399421466396	4.545527445354235	5.094172090419204
9	79.13838150558705	6.545973592740337	8.69848893087923	5.617155970793384
10	34.28183392819651	28.61773042200294	15.734058249935847	21.366377399864696
11	23.907376303450206	26.915226164648796	28.415506100263606	20.761891431637387
12	22.096688594956493	21.326355354935032	36.360793267548466	20.21616278256001
13	25.073483098887255	23.582315650733666	29.754531457764717	21.589669792614366
14	20.305902559078078	27.85213567546131	29.993132829449227	21.848828936011383
15	19.658114050435067	30.919763454405487	29.332951337858958	20.089171157300488
16	19.90189131499755	29.273319219912754	28.1314151911727	22.693374273917
17	18.7109256537663	30.07747801339025	29.313122565143352	21.898473767700096
18	20.24452084353932	29.2133226024681	28.555619707467283	21.986536846525297
19	19.791010567569458	32.29800895793967	28.832566426388595	19.078414048102267
20	20.49150570368815	30.131934238458484	28.869745375230366	20.506814682623
21	20.68031644388457	30.86297443254718	28.05319359880561	20.40351552476264
22	18.66959141064222	30.035997970466795	28.67473814356031	22.619672475330674
23	19.280419670142535	29.559961625492804	27.887419226911142	23.27219947745352
24	17.273339631884667	30.010264305876316	28.78984708517041	23.926548977068606
25	19.982081204656264	30.320599178855527	27.421515967994026	22.27580364849418
26	20.581537079804978	30.578227424359067	27.426983460470762	21.4132520353652
27	19.31993141577437	28.994185504000747	27.890262322999043	23.795620757225837
28	21.171807567592786	29.200710919821777	29.13714220729232	20.490339305293116
29	19.822649124034804	31.706353372057762	27.253481699209182	21.21751580469825
30	22.110539575897544	29.24838745421886	26.69536006718455	21.945712902699047
31	19.950515548090607	31.941601348356546	26.61159808244104	21.49628502111181
32	21.71389122168568	29.139037604684255	26.96487099633751	22.182200177292554
33	20.921906711456366	28.280787085636973	28.238286444117854	22.55901975878881
34	19.72591095714652	29.226881983810387	29.314143163739008	21.73306389530408
35	20.749425548790445	27.751971213287614	28.876233466302754	22.622369771619194
36	20.173953740639654	30.62590395875615	29.27186122191896	19.928281078685238
37	19.91909569132433	29.54049735227564	28.34231460097511	22.19809235542492
38	19.557220589264467	28.349969090442535	30.970793384188305	21.122016936104696
39	18.851185060769357	27.81568572561644	29.442519887092633	23.890609326521567
40	19.959992535050272	29.656189493083257	27.134217463316773	23.2496005085497
41	18.005181724869946	28.98580201553643	29.431584902139175	23.57743135745445
42	20.86861688478317	29.3433031236149	27.20609676441085	22.58198322719108
43	19.216267758415565	27.3478141694077	29.71348881423939	23.722429257937343
44	21.369001796253528	25.983784146313017	28.607232836447615	24.03998122098584
45	21.698363543051766	26.367893717778244	30.13149683906035	21.80224590010964
46	19.106845008981267	27.599391723236987	31.30649742225955	21.987265845522195
47	19.74996792404414	28.22188396668766	28.230486154851054	23.79766195441715
48	19.63821237781977	27.107098700632186	31.070374647164485	22.18431427438356
49	19.89081053024471	25.986627242400917	29.682068957473113	24.440493269881262
50	19.02884211631325	25.59464448176919	29.117313434576715	26.259199967340844
51	19.408213194298646	24.92010170994005	31.26319488184384	24.408490213917467
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1658.0
1	1327.0
2	996.0
3	749.5
4	503.0
5	608.0
6	713.0
7	722.0
8	731.0
9	819.0
10	907.0
11	1201.0
12	1495.0
13	1555.5
14	1616.0
15	1854.0
16	2092.0
17	2751.0
18	3410.0
19	4198.0
20	4986.0
21	6244.0
22	7502.0
23	9381.0
24	11260.0
25	14368.5
26	21253.0
27	25029.0
28	29724.5
29	34420.0
30	37336.0
31	40252.0
32	45467.5
33	50683.0
34	53283.0
35	55883.0
36	60431.5
37	64980.0
38	66171.0
39	67362.0
40	70189.5
41	73017.0
42	72618.0
43	72219.0
44	82234.0
45	92249.0
46	100287.0
47	108325.0
48	118267.0
49	128209.0
50	122125.5
51	116042.0
52	101496.0
53	86950.0
54	76054.0
55	65158.0
56	57542.5
57	49927.0
58	46045.0
59	42163.0
60	39879.0
61	37595.0
62	34902.5
63	32210.0
64	27195.5
65	22181.0
66	19275.5
67	16370.0
68	13937.5
69	11505.0
70	9732.0
71	7959.0
72	6788.0
73	5617.0
74	4884.5
75	3341.5
76	2531.0
77	2114.5
78	1698.0
79	1204.5
80	711.0
81	609.0
82	507.0
83	416.5
84	326.0
85	202.5
86	79.0
87	66.0
88	53.0
89	36.5
90	20.0
91	13.5
92	7.0
93	5.0
94	3.0
95	3.0
96	3.0
97	2.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1371744.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.143148396882545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.7136944519462	21.482491362955255
2	10.433611853110174	6.08136597101325
3	3.914603789131913	3.4225163742501015
4	1.9981636570288446	2.3293111991219653
5	1.2649318770112397	1.8432048701842871
6	0.9202291703774691	1.6091025162870416
7	0.7095349803370594	1.4474658257319444
8	0.5886146953714395	1.3723268332636538
9	0.4871917350119724	1.277847092806973
>10	4.8865378715108685	31.059154100353403
>50	0.8939046539584199	18.241659549070697
>100	0.18241239775075763	7.442868341240001
>500	0.004547677467901895	0.9039530498817033
>1k	0.0017685412375174037	0.9216242017894666
>5k	2.5264874821677197E-4	0.5651087120503441
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGCT	7675	0.5595067301187393	TruSeq Adapter, Index 13 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCT	2778	0.2025159213380922	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGT	1936	0.1411342057993328	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCC	1840	0.13413581542911795	No Hit
GGCCCTGGGAATGATACCTGTCTCTTATACACATCTGACGCGAGTTCACTC	1837	0.13391711573004877	No Hit
CCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	1714	0.12495042806821097	TruSeq Adapter, Index 19 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.289989968973803E-5	0.0	0.0	0.6477885086430121	0.0
2	7.289989968973803E-5	0.0	0.0	0.9648301723936827	0.0
3	7.289989968973803E-5	0.0	0.0	1.3624991252012038	0.0
4	7.289989968973803E-5	0.0	0.0	2.935970560104509	0.0
5	7.289989968973803E-5	0.0	0.0	3.204387990762125	0.0
6	7.289989968973803E-5	0.0	0.0	4.629216530198055	0.0
7	7.289989968973803E-5	0.0	0.0	5.675184290946416	0.0
8	7.289989968973803E-5	0.0	0.0	6.610344204166375	0.0
9	7.289989968973803E-5	0.0	0.0	7.904827722957053	0.0
10	7.289989968973803E-5	0.0	0.0	9.86437702661721	0.0
11	7.289989968973803E-5	0.0	0.0	11.589042853477034	0.0
12	7.289989968973803E-5	0.0	0.0	12.410114423682552	0.0
13	7.289989968973803E-5	0.0	0.0	12.781758112300837	0.0
14	7.289989968973803E-5	0.0	0.0	13.099528775048405	0.0
15	7.289989968973803E-5	0.0	0.0	13.373267898383371	0.0
16	7.289989968973803E-5	0.0	0.0	14.011652319966407	0.0
17	7.289989968973803E-5	0.0	0.0	14.843804674924767	0.0
18	7.289989968973803E-5	0.0	0.0	15.907487111297735	0.0
19	7.289989968973803E-5	0.0	0.0	16.360122588471317	0.0
20	7.289989968973803E-5	0.0	0.0	16.876472577973733	0.0
21	7.289989968973803E-5	7.289989968973803E-5	0.0	17.406746448316888	0.0
22	7.289989968973803E-5	7.289989968973803E-5	0.0	18.019105605710685	0.0
23	7.289989968973803E-5	7.289989968973803E-5	0.0	18.612364989385775	0.0
24	7.289989968973803E-5	7.289989968973803E-5	0.0	19.080746844892342	0.0
25	7.289989968973803E-5	7.289989968973803E-5	0.0	19.503347563393753	0.0
26	7.289989968973803E-5	7.289989968973803E-5	0.0	19.86303566846292	0.0
27	7.289989968973803E-5	7.289989968973803E-5	0.0	20.30582965917839	0.0
28	7.289989968973803E-5	7.289989968973803E-5	0.0	20.720848788112068	0.0
29	7.289989968973803E-5	7.289989968973803E-5	0.0	21.15168719527842	0.0
30	7.289989968973803E-5	7.289989968973803E-5	0.0	21.773523339631886	0.0
31	7.289989968973803E-5	7.289989968973803E-5	0.0	22.186428371474562	0.0
32	7.289989968973803E-5	7.289989968973803E-5	0.0	22.614059183054565	0.0
33	7.289989968973803E-5	7.289989968973803E-5	0.0	23.0215696223202	0.0
34	7.289989968973803E-5	7.289989968973803E-5	0.0	23.4030547973966	0.0
35	7.289989968973803E-5	7.289989968973803E-5	0.0	23.812533533953857	0.0
36	7.289989968973803E-5	7.289989968973803E-5	0.0	24.189353115450114	0.0
37	7.289989968973803E-5	7.289989968973803E-5	0.0	24.572660788018755	0.0
38	7.289989968973803E-5	7.289989968973803E-5	0.0	24.9609256537663	0.0
39	1.4579979937947606E-4	7.289989968973803E-5	0.0	25.36165640236079	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTAG	45	1.9288564E-8	40.0	1
CCGATAG	80	0.0	39.375	1
CGACGTC	35	6.2491745E-6	38.571426	18
CCATGCG	65	9.094947E-12	38.07692	1
TAAGGGA	885	0.0	36.864407	4
TTACGGG	855	0.0	36.57895	3
CACTAGG	310	0.0	36.290325	2
TGAGCGG	255	0.0	36.17647	2
CGACAGG	300	0.0	36.000004	2
GCGACGT	25	0.0021072507	35.999996	17
CGCGCAT	25	0.0021072507	35.999996	23
ACGGCAC	25	0.0021072507	35.999996	24
ACTACGT	25	0.0021072507	35.999996	16
TAGTGCG	25	0.0021072507	35.999996	1
ACGACTC	25	0.0021072507	35.999996	33
CGAGGGA	735	0.0	35.510204	4
TAAGGGC	850	0.0	35.47059	4
GTAAGGG	870	0.0	35.431034	3
CGACACG	70	2.1827873E-11	35.357143	1
CATGCGG	255	0.0	35.294117	2
>>END_MODULE