FastQCFastQC Report
Sat 18 Jun 2016
SRR3553137_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553137_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences746312
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGCT49200.6592417112414111TruSeq Adapter, Index 16 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCT20440.273880093044196No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCC15280.204740108694487No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGT14370.19254681688087558No Hit
CCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC11200.15007128385983343TruSeq Adapter, Index 13 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7490.1003601710812636No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCGC302.1644737E-645.0000048
CGCCACG351.2111559E-745.0000041
TGCCCCG207.0316944E-445.01
TAGCGCG207.0316944E-445.01
CCCCGGC253.889486E-545.03
CGCCGAG207.0316944E-445.01
TCCCCGG2700.042.52
TGCGGGC2600.040.673084
GTACCGG850.039.7058832
CAGTGCG403.4560435E-739.3751
AGGGCTC5050.039.2079246
CGGGCGC1400.038.571436
CCGAGCG356.2453437E-638.571431
GCGATAG356.2453437E-638.571431
GTTGGGC1400.038.571434
TACGTGG356.2453437E-638.571432
ACGGGTA2400.038.4375045
CGGGATC3650.038.2191776
CAAGGGC8750.037.84
ACAGGGC5800.037.629314
Produced by FastQC (version 0.11.5)