##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553137_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 746312 Sequences flagged as poor quality 0 Sequence length 51 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.62625014739144 31.0 31.0 34.0 30.0 34.0 2 31.830766489082315 33.0 31.0 34.0 30.0 34.0 3 31.995262035180996 33.0 31.0 34.0 30.0 34.0 4 35.681788581719175 37.0 35.0 37.0 33.0 37.0 5 35.454100965815904 37.0 35.0 37.0 33.0 37.0 6 35.4770417734138 37.0 35.0 37.0 33.0 37.0 7 35.716471127356925 37.0 35.0 37.0 35.0 37.0 8 35.8046956232782 37.0 35.0 37.0 35.0 37.0 9 37.48102670196915 39.0 37.0 39.0 35.0 39.0 10 36.94051683478224 39.0 37.0 39.0 33.0 39.0 11 36.91051329738769 39.0 37.0 39.0 33.0 39.0 12 36.91967836508056 39.0 37.0 39.0 33.0 39.0 13 36.919750720878135 39.0 37.0 39.0 33.0 39.0 14 38.07206771430715 40.0 37.0 41.0 33.0 41.0 15 38.04634656819132 40.0 37.0 41.0 33.0 41.0 16 37.96707141249236 40.0 37.0 41.0 33.0 41.0 17 37.93981203571697 40.0 37.0 41.0 33.0 41.0 18 37.872639057123564 40.0 37.0 41.0 33.0 41.0 19 37.95751508752372 40.0 37.0 41.0 33.0 41.0 20 37.86313230927548 40.0 37.0 41.0 33.0 41.0 21 37.87199991424498 40.0 37.0 41.0 33.0 41.0 22 37.85245580936659 40.0 37.0 41.0 33.0 41.0 23 37.72090224999732 39.0 37.0 41.0 33.0 41.0 24 37.59007760829251 39.0 36.0 41.0 32.0 41.0 25 37.64894976899742 39.0 37.0 41.0 32.0 41.0 26 37.53897163652735 39.0 36.0 41.0 32.0 41.0 27 37.525202596233214 39.0 37.0 41.0 32.0 41.0 28 37.45591656036618 39.0 36.0 41.0 32.0 41.0 29 37.26328934815466 39.0 36.0 41.0 31.0 41.0 30 37.332013956629396 39.0 36.0 41.0 32.0 41.0 31 37.21705801327059 39.0 36.0 41.0 31.0 41.0 32 37.169442538777346 39.0 36.0 41.0 31.0 41.0 33 37.13172506940797 39.0 36.0 41.0 31.0 41.0 34 37.13623524745683 39.0 36.0 41.0 31.0 41.0 35 37.101140005788466 39.0 36.0 41.0 31.0 41.0 36 37.06184544801638 39.0 36.0 41.0 31.0 41.0 37 37.01546806161498 39.0 36.0 41.0 31.0 41.0 38 36.93252151915017 39.0 35.0 40.0 31.0 41.0 39 36.86169859254574 39.0 35.0 40.0 31.0 41.0 40 36.82604058356291 39.0 35.0 40.0 31.0 41.0 41 36.77111315374803 39.0 35.0 40.0 31.0 41.0 42 36.707391814683405 39.0 35.0 40.0 31.0 41.0 43 36.61405417573347 39.0 35.0 40.0 30.0 41.0 44 36.54340275916775 39.0 35.0 40.0 30.0 41.0 45 36.321734073685 39.0 35.0 40.0 30.0 41.0 46 36.17871078047787 38.0 35.0 40.0 30.0 41.0 47 36.08041944923839 38.0 35.0 40.0 29.0 41.0 48 36.0249962482179 38.0 35.0 40.0 29.0 41.0 49 35.872018673155466 38.0 35.0 40.0 29.0 41.0 50 35.798704831223404 38.0 34.0 40.0 28.0 41.0 51 34.61868092701176 37.0 33.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 2.0 11 5.0 12 5.0 13 8.0 14 13.0 15 38.0 16 58.0 17 84.0 18 172.0 19 293.0 20 554.0 21 886.0 22 1326.0 23 1853.0 24 2557.0 25 3293.0 26 4375.0 27 5756.0 28 7667.0 29 10172.0 30 13497.0 31 17689.0 32 23106.0 33 30922.0 34 43885.0 35 56697.0 36 71893.0 37 105410.0 38 162963.0 39 181072.0 40 59.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 19.46183365670122 31.246449206230103 22.84111738790211 26.45059974916657 2 20.51206465928459 29.04495706889344 23.36864474911297 27.074333522708997 3 20.250913826924933 29.738232803438773 23.273778258958718 26.737075110677573 4 18.982409501656143 28.959470033980427 21.40699868151658 30.65112178284685 5 17.611669114257843 33.426636580947374 20.972193934976257 27.989500369818522 6 19.996462605451875 33.83705474386048 21.626075957508387 24.54040669317926 7 84.39071058752907 4.079795045503757 6.298304194492384 5.231190172474783 8 87.1193281094234 2.5359367127957206 4.533358702526558 5.811376475254317 9 78.41733752103679 5.911200677464653 9.622919100858622 6.048542700639946 10 32.38163127485556 25.89520200666745 16.166563046018286 25.556603672458706 11 20.027414807747963 25.03336406221527 34.1114708057756 20.827750324261167 12 18.40610897319084 20.30250083075175 41.857292928426716 19.434097267630694 13 22.99708432934215 24.160940732562253 32.057772084597325 20.784202853498268 14 18.826174575780637 29.43353450031622 30.95059438947786 20.789696534425282 15 17.595590048130006 34.867990867090434 28.637352742552714 18.899066342226845 16 17.78358112960799 32.618127539152525 28.333860369389747 21.264430961849737 17 15.990497271918446 33.28339890019188 31.32135085594229 19.40475297194739 18 16.09742306166858 32.76967273740741 30.932907416737237 20.199996784186773 19 15.948691699986064 36.333329760207526 31.55489929144915 16.163079248357256 20 15.765658330564161 34.00561695376732 31.384327198276324 18.84439751739219 21 15.394365895228807 34.923999614102414 30.263616289165924 19.418018201502857 22 14.445299017033092 33.40680573272304 31.81471020163149 20.333185048612375 23 15.379492759060554 32.27095370300893 30.348433362990278 22.00112017494024 24 13.504271671901297 33.740312362657974 31.12237241261028 21.633043552830454 25 15.412052867969429 32.72197150789482 30.135787713449602 21.730187910686148 26 16.894676757173944 33.746208020238186 30.099475822444234 19.259639400143637 27 14.348824620266054 32.21561491708562 31.08699846712903 22.3485619955193 28 16.32614777733709 32.77329052728618 30.009567044346063 20.890994651030667 29 16.169644867026122 35.79401108383625 28.459009100751427 19.577334948386195 30 15.360465864142611 33.92481964647494 29.112757131065827 21.60195735831663 31 14.807613973780404 37.02915134688977 26.258856885592085 21.90437779373774 32 15.125711498676155 34.37851729571547 28.797741427177908 21.69802977843047 33 14.526096324325483 32.28957862127368 30.076831137647524 23.107493916753317 34 12.844226007353493 33.051592363515525 29.448005659831278 24.656175969299703 35 13.186576123658739 33.027473764323766 29.883748351895722 23.902201760121773 36 13.649251251487312 33.70962814479735 31.58384161047926 21.05727899323607 37 14.05015596694144 34.3715497003934 28.729003419481398 22.84929091318376 38 15.389542175390453 34.725557139641325 29.034103699257148 20.85079698571107 39 14.417428635744836 33.35200291567066 28.624891466303637 23.60567698228087 40 15.35765202757024 32.07237723633011 27.44321409812518 25.126756637974466 41 14.381250736957195 30.01693661632132 30.363172506940796 25.23864013978068 42 16.47836293668064 31.066765642251497 29.22383667956565 23.231034741502214 43 14.629538316414584 30.809232599770603 31.993723804521434 22.56750527929338 44 17.012991885431294 30.386353160608433 30.037303433416586 22.563351520543687 45 17.571739433373708 31.211209252966587 30.57367428099776 20.643377032661945 46 14.606089678311484 31.499828489961303 32.37439569509803 21.519686136629186 47 15.64868312448413 30.769436911104204 29.27167190129597 24.31020806311569 48 16.206626719120155 29.46019895164489 32.23959952405964 22.093574805175315 49 16.606459496832425 28.77616868012306 30.60074070897962 24.016631114064896 50 15.671997770369497 28.88269249321999 29.073899387923554 26.37141034848696 51 16.20300892924139 27.711198533589172 32.59936862867004 23.486423908499393 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 941.0 1 712.5 2 484.0 3 383.5 4 283.0 5 357.5 6 432.0 7 454.0 8 476.0 9 574.0 10 672.0 11 847.0 12 1022.0 13 1106.5 14 1191.0 15 1454.0 16 1717.0 17 2440.0 18 3163.0 19 3628.5 20 4094.0 21 5308.0 22 6522.0 23 8295.0 24 10068.0 25 12825.5 26 17803.0 27 20023.0 28 23212.5 29 26402.0 30 29533.5 31 32665.0 32 35346.0 33 38027.0 34 40197.5 35 42368.0 36 43558.5 37 44749.0 38 45063.0 39 45377.0 40 45862.0 41 46347.0 42 47504.0 43 48661.0 44 53145.5 45 57630.0 46 62191.0 47 66752.0 48 70259.0 49 73766.0 50 65976.5 51 58187.0 52 48811.0 53 39435.0 54 31383.5 55 23332.0 56 18084.0 57 12836.0 58 10462.0 59 8088.0 60 6550.0 61 5012.0 62 4406.0 63 3800.0 64 3072.5 65 2345.0 66 1892.0 67 1439.0 68 1169.5 69 900.0 70 729.5 71 559.0 72 472.5 73 386.0 74 326.0 75 210.5 76 155.0 77 111.0 78 67.0 79 56.0 80 45.0 81 33.0 82 21.0 83 16.0 84 11.0 85 7.0 86 3.0 87 3.0 88 3.0 89 3.0 90 3.0 91 2.5 92 2.0 93 2.0 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 746312.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.02634319962065 #Duplication Level Percentage of deduplicated Percentage of total 1 74.61678188475351 31.358704839398875 2 9.861579089885302 8.288922146434446 3 4.005601042436387 5.050222923905695 4 2.28352788871104 3.8387330702750124 5 1.5640936676784924 3.286656863710486 6 1.1669927886205451 2.9426663667629627 7 0.8849243477950784 2.60330940422955 8 0.7441339450455334 2.501858284877699 9 0.6107815921670496 2.310202513118084 >10 4.141130033427689 30.88266358724836 >50 0.08585532720900313 2.3688811591797276 >100 0.03043377097860705 2.4189636009117588 >500 0.002562843871881019 0.6606475421422463 >1k 0.001601777419925637 1.4875676978051109 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGCT 4920 0.6592417112414111 TruSeq Adapter, Index 16 (95% over 22bp) AATCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCT 2044 0.273880093044196 No Hit AATGATACGGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCC 1528 0.204740108694487 No Hit AATGATACCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGT 1437 0.19254681688087558 No Hit CCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC 1120 0.15007128385983343 TruSeq Adapter, Index 13 (95% over 23bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 749 0.1003601710812636 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.7693833142171103 0.0 2 0.0 0.0 0.0 1.1572907845512332 0.0 3 0.0 0.0 0.0 1.6635133831427071 0.0 4 0.0 0.0 0.0 3.671922734727567 0.0 5 0.0 0.0 0.0 4.023652306274052 0.0 6 0.0 0.0 0.0 5.89619354907867 0.0 7 0.0 0.0 0.0 7.342639539495546 0.0 8 0.0 0.0 0.0 8.731066899634468 0.0 9 0.0 0.0 0.0 10.482747162044829 0.0 10 0.0 0.0 0.0 13.00729453633333 0.0 11 0.0 0.0 0.0 15.251128214473303 0.0 12 0.0 0.0 0.0 16.322262003022864 0.0 13 0.0 0.0 0.0 16.76952802581226 0.0 14 0.0 0.0 0.0 17.15984735606556 0.0 15 0.0 0.0 0.0 17.48933421946853 0.0 16 0.0 0.0 0.0 18.18997952598913 0.0 17 0.0 0.0 0.0 19.26097932232096 0.0 18 0.0 0.0 0.0 20.43408118856457 0.0 19 0.0 0.0 0.0 21.051383335655864 0.0 20 0.0 0.0 0.0 21.64952459561149 0.0 21 0.0 0.0 0.0 22.272320423629797 0.0 22 0.0 0.0 0.0 22.973233714585856 0.0 23 0.0 0.0 0.0 23.701615410176977 0.0 24 0.0 0.0 0.0 24.28555349505301 0.0 25 0.0 0.0 0.0 24.804237369893556 0.0 26 0.0 0.0 0.0 25.2533792837312 0.0 27 0.0 0.0 0.0 25.77809280836969 0.0 28 0.0 0.0 0.0 26.25912487002755 0.0 29 0.0 0.0 0.0 26.76722335966727 0.0 30 0.0 0.0 0.0 27.48207184126746 0.0 31 0.0 0.0 0.0 27.938583327080362 0.0 32 0.0 0.0 0.0 28.402062408215333 0.0 33 0.0 0.0 0.0 28.841824866811734 0.0 34 0.0 0.0 0.0 29.288822905165667 0.0 35 0.0 0.0 0.0 29.74520039876084 0.0 36 0.0 0.0 0.0 30.19595021921127 0.0 37 0.0 0.0 0.0 30.637990545509115 0.0 38 0.0 0.0 0.0 31.102943541039135 0.0 39 0.0 0.0 0.0 31.55556925253781 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCCCGC 30 2.1644737E-6 45.000004 8 CGCCACG 35 1.2111559E-7 45.000004 1 TGCCCCG 20 7.0316944E-4 45.0 1 TAGCGCG 20 7.0316944E-4 45.0 1 CCCCGGC 25 3.889486E-5 45.0 3 CGCCGAG 20 7.0316944E-4 45.0 1 TCCCCGG 270 0.0 42.5 2 TGCGGGC 260 0.0 40.67308 4 GTACCGG 85 0.0 39.705883 2 CAGTGCG 40 3.4560435E-7 39.375 1 AGGGCTC 505 0.0 39.207924 6 CGGGCGC 140 0.0 38.57143 6 CCGAGCG 35 6.2453437E-6 38.57143 1 GCGATAG 35 6.2453437E-6 38.57143 1 GTTGGGC 140 0.0 38.57143 4 TACGTGG 35 6.2453437E-6 38.57143 2 ACGGGTA 240 0.0 38.437504 5 CGGGATC 365 0.0 38.219177 6 CAAGGGC 875 0.0 37.8 4 ACAGGGC 580 0.0 37.62931 4 >>END_MODULE