FastQCFastQC Report
Sat 18 Jun 2016
SRR3553136_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553136_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751229
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCT73170.9740039322230639TruSeq Adapter, Index 13 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCT43040.5729278289309917No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGT40000.5324608075566838No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCC38860.5172856745413182No Hit
CCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC14450.192351466729852TruSeq Adapter, Index 16 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTT11710.1558779014122192No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11340.15095263894231986No Hit
AACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTG10420.13870604036851614No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCT9960.13258274108161425No Hit
ACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTG9840.1309853586589442No Hit
TACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCT9110.12126794892103473No Hit
AACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCT7850.1044954334829992No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAC207.0317235E-445.0000049
CGACCGT207.0317235E-445.00000411
CGCTACG207.0317235E-445.0000041
GTGCGCT207.0317235E-445.00000444
GTCGGAC207.0317235E-445.00000423
GCCGTGA253.88951E-545.022
CGCGAGG750.042.0000042
GCGATAG700.041.7857171
ACCGGAG1250.039.6000021
CGGCACT403.45608E-739.3750046
CACAGGG7100.039.2957763
TGCGGGT1800.038.754
TGCGCGT356.2453983E-638.5714341
CGCTAAG356.2453983E-638.571431
CGCGTAG356.2453983E-638.571431
TCACAGG2750.038.4545442
CGATAGG1350.038.3333362
AGGGCTC4550.038.0769236
AGGGATC4500.038.06
TATGGGC4100.037.8658524
Produced by FastQC (version 0.11.5)