##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553136_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	751229
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.522730086298587	31.0	31.0	34.0	30.0	34.0
2	31.72460195226755	31.0	31.0	34.0	30.0	34.0
3	31.905071556076777	33.0	31.0	34.0	30.0	34.0
4	35.606036241944864	37.0	35.0	37.0	33.0	37.0
5	35.365556973971984	37.0	35.0	37.0	33.0	37.0
6	34.703166411307336	37.0	35.0	37.0	32.0	37.0
7	35.23474200277146	37.0	35.0	37.0	33.0	37.0
8	35.68900029152229	37.0	35.0	37.0	35.0	37.0
9	37.36509506422143	39.0	37.0	39.0	34.0	39.0
10	36.81263236642888	39.0	37.0	39.0	32.0	39.0
11	36.80896770492087	39.0	37.0	39.0	32.0	39.0
12	36.74811808383329	39.0	37.0	39.0	32.0	39.0
13	36.768402178297166	39.0	37.0	39.0	32.0	39.0
14	37.885217423715005	40.0	37.0	41.0	33.0	41.0
15	37.880104468810444	40.0	37.0	41.0	33.0	41.0
16	37.76577049075581	39.0	37.0	41.0	33.0	41.0
17	37.76564536246604	39.0	37.0	41.0	33.0	41.0
18	37.74111622421392	39.0	37.0	41.0	33.0	41.0
19	37.78755080008892	40.0	37.0	41.0	33.0	41.0
20	37.73399456091285	39.0	37.0	41.0	33.0	41.0
21	37.758277436041475	39.0	37.0	41.0	33.0	41.0
22	37.739633320864876	39.0	37.0	41.0	33.0	41.0
23	37.655051921584494	39.0	37.0	41.0	32.0	41.0
24	37.50295049844987	39.0	36.0	41.0	32.0	41.0
25	37.585689583336105	39.0	36.0	41.0	32.0	41.0
26	37.541786858601036	39.0	36.0	41.0	32.0	41.0
27	37.48343580985292	39.0	36.0	41.0	32.0	41.0
28	37.47305814871364	39.0	36.0	41.0	32.0	41.0
29	37.252606062864984	39.0	36.0	41.0	31.0	41.0
30	37.29125739288553	39.0	36.0	41.0	31.0	41.0
31	37.28731052714951	39.0	36.0	41.0	32.0	41.0
32	37.24608741142847	39.0	36.0	41.0	31.0	41.0
33	37.09427085482589	39.0	36.0	41.0	31.0	41.0
34	37.11873210432505	39.0	36.0	41.0	31.0	41.0
35	37.078609851323634	39.0	36.0	41.0	31.0	41.0
36	37.099454360787455	39.0	36.0	41.0	31.0	41.0
37	36.9701169683279	39.0	36.0	40.0	31.0	41.0
38	36.82054739633321	39.0	35.0	40.0	31.0	41.0
39	36.75526237671868	39.0	35.0	40.0	31.0	41.0
40	36.703724164003255	39.0	35.0	40.0	30.0	41.0
41	36.7458990534178	39.0	35.0	40.0	31.0	41.0
42	36.694927911462415	39.0	35.0	40.0	31.0	41.0
43	36.61181743516291	39.0	35.0	40.0	31.0	41.0
44	36.46150241803764	39.0	35.0	40.0	30.0	41.0
45	36.218391462523414	38.0	35.0	40.0	29.0	41.0
46	36.04675937696761	38.0	35.0	40.0	29.0	41.0
47	35.97944701282831	38.0	35.0	40.0	29.0	41.0
48	35.910060713843585	38.0	35.0	40.0	29.0	41.0
49	35.80376689398306	38.0	34.0	40.0	28.0	41.0
50	35.692290899312994	38.0	34.0	40.0	28.0	41.0
51	34.41697937646177	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	5.0
11	2.0
12	12.0
13	12.0
14	20.0
15	27.0
16	54.0
17	121.0
18	220.0
19	414.0
20	738.0
21	1141.0
22	1716.0
23	2183.0
24	2854.0
25	3598.0
26	4578.0
27	5920.0
28	7641.0
29	10481.0
30	13789.0
31	18714.0
32	24356.0
33	32463.0
34	46097.0
35	58196.0
36	73943.0
37	107058.0
38	160763.0
39	174067.0
40	43.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.122131866581295	32.505534264518545	22.389710727354775	25.982623141545385
2	19.632761781028155	30.369035274197348	23.513469261703158	26.484733683071344
3	20.17467376791897	28.770454814710295	24.490002382762114	26.564869034608623
4	20.019195212112418	28.29576600477351	21.922742599127563	29.762296183986507
5	17.44647770520041	33.48912249127763	21.366587285634605	27.697812517887353
6	19.934800174114685	35.21589289018395	22.77920580808249	22.070101127618873
7	81.54783694452689	5.394759786962431	7.217905592036517	5.839497676474151
8	84.95971268414824	2.688128386949918	5.030955940199327	7.321202988702513
9	76.54350404470541	6.265466322519499	10.472971623832414	6.718058008942679
10	31.817062440347748	25.63998461188266	16.877543332326095	25.66540961544349
11	20.56683115268447	24.912776263962122	33.338302967537196	21.182089615816217
12	18.94615356968381	20.854626219168857	41.22857344431591	18.97064676683142
13	23.090961610906927	24.375390193935537	30.89244424802557	21.641203947131967
14	18.980763522174996	27.975490829028164	32.467463316778236	20.5762823320186
15	17.043404873880004	33.98923630477525	28.4692151128351	20.498143708509655
16	17.817469772865532	31.259309744432123	28.10195027082288	22.821270211879465
17	15.376536315823802	32.2146775483907	31.763816359592084	20.644969776193413
18	16.035722795578977	31.082399641121416	31.119272552044713	21.76260501125489
19	15.869062562813735	35.11792010159352	32.25461210895745	16.758405226635286
20	16.4226886874708	32.96145383098895	31.759556673131627	18.856300808408623
21	16.040781173250767	32.67312630369701	30.425609235000245	20.860483288051977
22	14.701642242245708	32.291751250284534	31.838893333457573	21.167713174012185
23	16.668552465360094	31.340510017584517	28.61590806531697	23.37502945173842
24	13.555653469181832	33.91895147817776	29.807156007023156	22.718239045617246
25	17.057515085280254	31.19874232757255	29.99311794406233	21.750624643084866
26	17.87084896882309	31.624977204021675	29.69800154147404	20.806172285681196
27	15.04015420064987	29.769484404888523	31.48427443562482	23.706086958836785
28	20.55711374294656	29.22757241799771	29.7009300759156	20.514383763140135
29	17.05538524205003	31.50677090474409	29.48049130158713	21.957352551618747
30	18.42780297352738	30.751874594830603	29.88755758896422	20.9327648426778
31	19.320074171790495	31.62724016245379	27.26864910699667	21.784036558759045
32	16.51413883116866	29.345246256467732	31.78338429426979	22.35723061809382
33	18.857232614821847	27.819479812414055	30.439186985592944	22.884100587171154
34	17.22031497719071	26.363199503746525	33.68453560765093	22.731949911411835
35	18.073982766905964	25.988746440832287	33.469421441398026	22.467849350863716
36	17.52834355436225	26.45917556430862	35.515668324838366	20.496812556490767
37	17.294593259844866	28.60911919002062	32.933632753794114	21.162654796340398
38	19.35588216109868	27.85049565445423	33.42323046634249	19.3703917181046
39	16.738171715948134	26.87063465334805	34.53900208857752	21.852191542126302
40	17.17638696056728	28.492643388367593	32.328890391611615	22.00207925945351
41	16.263216675607573	26.553421127246153	36.4703705527875	20.712991644358777
42	18.922858409353207	27.500136443081935	32.37694497949361	21.200060168071253
43	16.998012590035792	26.321268215151438	33.05703054594537	23.62368864886739
44	19.060765758510385	25.418747146342856	31.647739903544725	23.872747191602027
45	21.146947202517474	26.808203623662024	31.660918308531755	20.383930865288747
46	16.085907226691194	26.577248748384314	35.99634731886016	21.34049670606433
47	17.673971585229005	26.705039342197917	32.00169322536803	23.619295847205045
48	17.528476669564142	24.99317784590318	37.248295792627815	20.230049691904863
49	18.942692574434695	24.173454432669665	33.422698005534926	23.46115498736071
50	17.333196668392727	24.606211954011357	30.894307860852017	27.166283516743896
51	17.761561388072078	23.958473381618653	35.39027380465877	22.8896914256505
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1410.0
1	1145.5
2	881.0
3	615.0
4	349.0
5	382.0
6	415.0
7	444.0
8	473.0
9	559.0
10	645.0
11	781.0
12	917.0
13	1123.5
14	1330.0
15	1388.0
16	1446.0
17	1908.0
18	2370.0
19	3117.5
20	3865.0
21	4431.0
22	4997.0
23	7081.0
24	9165.0
25	11020.5
26	15317.0
27	17758.0
28	20533.0
29	23308.0
30	26071.5
31	28835.0
32	30933.5
33	33032.0
34	36458.5
35	39885.0
36	40357.0
37	40829.0
38	40871.5
39	40914.0
40	41422.0
41	41930.0
42	43592.5
43	45255.0
44	54986.5
45	64718.0
46	67856.0
47	70994.0
48	75071.5
49	79149.0
50	70683.0
51	62217.0
52	51623.5
53	41030.0
54	32784.5
55	24539.0
56	19714.5
57	14890.0
58	13187.5
59	11485.0
60	10258.5
61	9032.0
62	7576.5
63	6121.0
64	5129.5
65	4138.0
66	3746.5
67	3355.0
68	2693.0
69	2031.0
70	1879.5
71	1728.0
72	1514.5
73	1301.0
74	961.5
75	551.5
76	481.0
77	373.5
78	266.0
79	209.5
80	153.0
81	103.5
82	54.0
83	37.0
84	20.0
85	13.5
86	7.0
87	6.5
88	6.0
89	4.5
90	3.0
91	3.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	751229.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.73033547613482
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.91497182865078	26.41006740146746
2	10.610693457766505	7.582472737608524
3	4.0245656960490175	4.3139724739672625
4	2.1554893423185986	3.080654292651069
5	1.3552149398912419	2.4211142222291975
6	1.0173707992821766	2.181059997718535
7	0.7448993452278514	1.8630852450661008
8	0.6361997165238497	1.8185303440975216
9	0.5375251668344095	1.728535908407291
>10	4.628581572227079	33.3471798179438
>50	0.31503956449216675	7.3459285679659505
>100	0.05263281103403604	3.5917877769190234
>500	0.0037865331679162594	1.028099868796431
>1k	0.002650573217541382	2.2975644126728505
>5k	3.786533167916259E-4	0.9899469324889439
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCT	7317	0.9740039322230639	TruSeq Adapter, Index 13 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCT	4304	0.5729278289309917	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGT	4000	0.5324608075566838	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCC	3886	0.5172856745413182	No Hit
CCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	1445	0.192351466729852	TruSeq Adapter, Index 16 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTT	1171	0.1558779014122192	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1134	0.15095263894231986	No Hit
AACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTG	1042	0.13870604036851614	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCT	996	0.13258274108161425	No Hit
ACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTG	984	0.1309853586589442	No Hit
TACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCT	911	0.12126794892103473	No Hit
AACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCT	785	0.1044954334829992	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.662304037783419E-4	0.0	0.0	1.0766357528796147	0.0
2	2.662304037783419E-4	0.0	0.0	1.538678618636927	0.0
3	2.662304037783419E-4	0.0	0.0	2.202391015256333	0.0
4	2.662304037783419E-4	0.0	0.0	4.844062196746931	0.0
5	2.662304037783419E-4	0.0	0.0	5.208398504317592	0.0
6	2.662304037783419E-4	0.0	0.0	7.0174340979914245	0.0
7	2.662304037783419E-4	0.0	0.0	8.374410466049634	0.0
8	2.662304037783419E-4	0.0	0.0	9.435471740308214	0.0
9	2.662304037783419E-4	0.0	0.0	11.531370594053211	0.0
10	2.662304037783419E-4	0.0	0.0	14.131776062958165	0.0
11	2.662304037783419E-4	0.0	0.0	16.86063770168617	0.0
12	2.662304037783419E-4	0.0	0.0	17.941666256228128	0.0
13	2.662304037783419E-4	0.0	0.0	18.37775165761705	0.0
14	2.662304037783419E-4	0.0	0.0	18.848580126699048	0.0
15	2.662304037783419E-4	0.0	0.0	19.20998789982815	0.0
16	2.662304037783419E-4	0.0	0.0	20.05460385581494	0.0
17	2.662304037783419E-4	0.0	0.0	21.328516337894303	0.0
18	2.662304037783419E-4	0.0	0.0	22.774946121622037	0.0
19	2.662304037783419E-4	0.0	0.0	23.4441162415189	0.0
20	2.662304037783419E-4	0.0	0.0	24.08133871296236	0.0
21	2.662304037783419E-4	0.0	0.0	24.78565124615796	0.0
22	2.662304037783419E-4	0.0	0.0	25.59232936960634	0.0
23	2.662304037783419E-4	0.0	0.0	26.380903825597787	0.0
24	5.324608075566837E-4	0.0	0.0	27.009473808918454	0.0
25	5.324608075566837E-4	0.0	0.0	27.563499279181183	0.0
26	6.655760094458548E-4	0.0	0.0	28.019019500045925	0.0
27	6.655760094458548E-4	0.0	0.0	28.54495766271004	0.0
28	6.655760094458548E-4	0.0	0.0	29.05199346670589	0.0
29	6.655760094458548E-4	0.0	0.0	29.5792627813889	0.0
30	6.655760094458548E-4	0.0	0.0	30.23552072670251	0.0
31	6.655760094458548E-4	0.0	0.0	30.71100822785063	0.0
32	6.655760094458548E-4	0.0	0.0	31.18742753541197	0.0
33	6.655760094458548E-4	0.0	0.0	31.649204170765504	0.0
34	6.655760094458548E-4	0.0	0.0	32.114042455762494	0.0
35	6.655760094458548E-4	0.0	0.0	32.590195532920056	0.0
36	6.655760094458548E-4	0.0	0.0	33.06155646280961	0.0
37	6.655760094458548E-4	0.0	0.0	33.52546294139337	0.0
38	6.655760094458548E-4	0.0	0.0	33.98697334634313	0.0
39	6.655760094458548E-4	0.0	0.0	34.462593962693134	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGAC	20	7.0317235E-4	45.000004	9
CGACCGT	20	7.0317235E-4	45.000004	11
CGCTACG	20	7.0317235E-4	45.000004	1
GTGCGCT	20	7.0317235E-4	45.000004	44
GTCGGAC	20	7.0317235E-4	45.000004	23
GCCGTGA	25	3.88951E-5	45.0	22
CGCGAGG	75	0.0	42.000004	2
GCGATAG	70	0.0	41.785717	1
ACCGGAG	125	0.0	39.600002	1
CGGCACT	40	3.45608E-7	39.375004	6
CACAGGG	710	0.0	39.295776	3
TGCGGGT	180	0.0	38.75	4
TGCGCGT	35	6.2453983E-6	38.57143	41
CGCTAAG	35	6.2453983E-6	38.57143	1
CGCGTAG	35	6.2453983E-6	38.57143	1
TCACAGG	275	0.0	38.454544	2
CGATAGG	135	0.0	38.333336	2
AGGGCTC	455	0.0	38.076923	6
AGGGATC	450	0.0	38.0	6
TATGGGC	410	0.0	37.865852	4
>>END_MODULE