FastQCFastQC Report
Sat 18 Jun 2016
SRR3553133_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553133_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058759
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGCT76030.7181048756138082No Hit
AATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCT38350.36221651952899575No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGT36690.3465377862195268No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCC32970.31140231157421094No Hit
CCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC16440.15527612988413794No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13740.12977457570608608No Hit
ACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTG11540.10899553156100682No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGAC302.1653395E-644.9999969
TCTAGCG750.042.01
TACGGGT2500.039.64
CGGGTCT3150.039.2857136
CGAGGGC3450.038.478264
TAGGGCC5000.038.255
TACGCAG659.094947E-1238.0769271
CCTAGCG950.037.8947371
CCACACG1250.037.81
TTCGCGG1800.037.52
CCGTACT301.13986134E-437.49999644
CACTCCG601.546141E-1037.4999961
CGGCTTA301.13986134E-437.49999631
CGCACTG1200.037.4999961
TACAGCG601.546141E-1037.4999961
GCCGTAC301.13986134E-437.49999643
GCCCCAG5450.037.155961
ACGGGTC2850.037.1052635
AGGGCGT3100.037.016136
CGATAGG1550.036.290322
Produced by FastQC (version 0.11.5)