##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553133_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1058759
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.66344182198215	31.0	31.0	34.0	30.0	34.0
2	31.88037976536681	33.0	31.0	34.0	30.0	34.0
3	32.02606825538201	33.0	31.0	34.0	30.0	34.0
4	35.71752684038577	37.0	35.0	37.0	33.0	37.0
5	35.48501028090434	37.0	35.0	37.0	33.0	37.0
6	35.50774633320709	37.0	35.0	37.0	33.0	37.0
7	35.68853440679135	37.0	35.0	37.0	35.0	37.0
8	35.761055159861684	37.0	35.0	37.0	35.0	37.0
9	37.42914865422632	39.0	37.0	39.0	35.0	39.0
10	36.929817833897985	39.0	37.0	39.0	33.0	39.0
11	36.920439873474514	39.0	37.0	39.0	33.0	39.0
12	36.88948854271841	39.0	37.0	39.0	33.0	39.0
13	36.84856988228672	39.0	37.0	39.0	33.0	39.0
14	38.009275954206764	40.0	37.0	41.0	33.0	41.0
15	38.01158053910286	40.0	37.0	41.0	33.0	41.0
16	37.91139248875334	40.0	37.0	41.0	33.0	41.0
17	37.86126210025133	40.0	37.0	41.0	33.0	41.0
18	37.80324889800228	40.0	37.0	41.0	33.0	41.0
19	37.88245294727129	40.0	37.0	41.0	33.0	41.0
20	37.816649492471846	40.0	37.0	41.0	33.0	41.0
21	37.80355586115443	39.0	37.0	41.0	33.0	41.0
22	37.77432541305434	39.0	37.0	41.0	33.0	41.0
23	37.65808366209874	39.0	37.0	41.0	33.0	41.0
24	37.54661353528046	39.0	36.0	41.0	32.0	41.0
25	37.6165567423748	39.0	36.0	41.0	32.0	41.0
26	37.552846304021976	39.0	36.0	41.0	32.0	41.0
27	37.541611452653534	39.0	36.0	41.0	32.0	41.0
28	37.430503070103775	39.0	36.0	41.0	32.0	41.0
29	37.312132411625306	39.0	36.0	41.0	32.0	41.0
30	37.32155381914109	39.0	36.0	41.0	32.0	41.0
31	37.19991990622984	39.0	36.0	41.0	31.0	41.0
32	37.21875610974735	39.0	36.0	41.0	31.0	41.0
33	37.114963839740675	39.0	36.0	41.0	31.0	41.0
34	37.09962040464355	39.0	36.0	41.0	31.0	41.0
35	37.05251053355863	39.0	36.0	41.0	31.0	41.0
36	36.95709788535446	39.0	35.0	41.0	31.0	41.0
37	36.834050997441345	39.0	35.0	41.0	31.0	41.0
38	36.760623522444675	39.0	35.0	40.0	30.0	41.0
39	36.6768981420701	39.0	35.0	40.0	30.0	41.0
40	36.578958006496286	39.0	35.0	40.0	30.0	41.0
41	36.60141920871511	39.0	35.0	40.0	30.0	41.0
42	36.5834576140557	39.0	35.0	40.0	30.0	41.0
43	36.54026553729413	39.0	35.0	40.0	30.0	41.0
44	36.44422479525558	39.0	35.0	40.0	30.0	41.0
45	36.22816240523103	38.0	35.0	40.0	30.0	41.0
46	36.1160991311526	38.0	35.0	40.0	30.0	41.0
47	35.95515787823291	38.0	35.0	40.0	29.0	41.0
48	35.90566408408335	38.0	35.0	40.0	29.0	41.0
49	35.75467410430513	38.0	34.0	40.0	28.0	41.0
50	35.66776480766634	38.0	34.0	40.0	28.0	41.0
51	34.41065436043519	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	2.0
10	3.0
11	5.0
12	4.0
13	15.0
14	23.0
15	31.0
16	75.0
17	113.0
18	264.0
19	520.0
20	907.0
21	1482.0
22	2157.0
23	3060.0
24	4012.0
25	5107.0
26	6461.0
27	8470.0
28	11174.0
29	14474.0
30	19228.0
31	25142.0
32	32920.0
33	45067.0
34	65550.0
35	81654.0
36	101427.0
37	147080.0
38	226602.0
39	255665.0
40	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.893762414298248	32.15670421691811	21.984700956497182	25.96483241228646
2	21.23967777369543	29.96479841021422	22.348900930240028	26.446622885850317
3	20.47907030778487	28.78228189795789	22.848353591327207	27.890294202930033
4	19.837470094705218	28.656757581281482	21.50631069015706	29.99946163385624
5	17.68192761525522	33.55286708306612	19.9332425981739	28.831962703504765
6	20.67014306371894	34.05165859274868	21.651291748169317	23.62690659536306
7	83.63603048474675	4.79060862764803	6.314845965890255	5.258514921714951
8	86.32946685695234	2.9475074119794966	4.5919798556612035	6.131045875406962
9	77.92292674725788	6.886647480682573	9.570072131618243	5.6203536404413095
10	33.10337857812779	28.76159730401347	15.57474363854286	22.560280479315878
11	22.079528957959273	25.9642657110825	31.328942658338676	20.627262672619548
12	21.156183796312476	20.876138951357202	38.731666035424496	19.23601121690583
13	24.187185185674927	24.77721558919452	30.179861517115793	20.85573770801476
14	19.374853011875224	28.072205289400138	31.278506251186535	21.27443544753811
15	18.594316553625518	32.80888285247162	28.464268072337518	20.13253252156534
16	19.141277665644402	30.505148008186943	27.90776749005203	22.445806836116624
17	17.69722854776205	31.137114300799336	30.107040412407354	21.05861673903126
18	18.39616003264199	30.101845651371086	29.903972480989538	21.59802183499739
19	18.186386136977347	33.28160610677217	30.74344586445074	17.78856189179974
20	18.834881214705142	31.56459590898401	30.340426858236864	19.26009601807399
21	18.787372763773437	31.622399431787596	29.36834539304979	20.221882411389185
22	16.812796868786947	31.444077452942548	30.184300676546787	21.558825001723715
23	18.640880502550626	29.83039577467582	28.313147751282397	23.215575971491152
24	15.585133160615399	31.845868606547857	29.702699103384244	22.8662991294525
25	17.523345728348	30.23851509172531	28.94766419931259	23.290474980614096
26	18.551530612726786	31.0048840198761	28.58478652837898	21.858798839018135
27	17.72131334893021	28.834985109925864	29.294768686736074	24.14893285440785
28	18.23899489874466	28.493169833739312	30.401913938866166	22.86592132864986
29	19.341889891845078	30.349588527700828	27.90568958563752	22.402831994816573
30	20.627451573020867	29.057509782679535	28.42252108364604	21.89251756065356
31	18.368675024250088	31.14448141645077	27.7795041175565	22.707339441742644
32	21.217104175737823	28.477207749827866	27.78025971916177	22.525428355272542
33	17.84154845436969	27.584275552793414	31.452011269797943	23.122164723038953
34	17.600228191684796	28.177422812934765	29.647445735998467	24.574903259381976
35	18.685933248265187	25.90476208466705	32.29412925887761	23.115175408190154
36	18.954738519341984	28.68499819127866	31.578196737878972	20.78206655150039
37	19.780611073908226	29.551673232529787	30.10807936461461	20.55963632894738
38	20.75750949932893	28.517632435710112	31.9377686517895	18.787089413171458
39	18.95568302134858	28.49675894136437	31.61777137195528	20.929786665331772
40	19.020098058198325	29.75001865391463	30.222836358415844	21.0070469294712
41	16.48023771226502	28.319287014325262	32.64916756315649	22.55130771025323
42	19.502266332564826	28.159855075612107	29.72045574110822	22.617422850714846
43	17.85902174149169	26.81715102303735	31.22721979222845	24.09660744324251
44	20.59118269596764	25.179478993803123	29.510020694038964	24.719317616190274
45	21.14872223046038	26.370401573918144	31.281245307005655	21.199630888615822
46	18.114981785278804	26.359823151444285	33.427720567192345	22.09747449608457
47	18.5971500596453	27.940919510483496	30.193840146813393	23.26809028305781
48	18.7730163332732	26.209930682997733	33.74904014983579	21.268012833893266
49	19.643374932350042	25.027319720540746	32.08076625558791	23.2485390915213
50	18.823074939622707	25.495698265610965	29.4323826290969	26.248844165669432
51	18.86047721908385	24.835963614004697	32.96482013376038	23.338739033151075
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1657.0
1	1353.5
2	1050.0
3	744.0
4	438.0
5	446.0
6	454.0
7	522.5
8	591.0
9	730.5
10	870.0
11	1014.5
12	1159.0
13	1363.0
14	1567.0
15	1676.0
16	1785.0
17	2346.0
18	2907.0
19	3361.0
20	3815.0
21	5132.0
22	6449.0
23	8114.5
24	9780.0
25	12402.5
26	19045.0
27	23065.0
28	25917.5
29	28770.0
30	32656.5
31	36543.0
32	39234.5
33	41926.0
34	44303.5
35	46681.0
36	49956.0
37	53231.0
38	55049.5
39	56868.0
40	57114.5
41	57361.0
42	58541.5
43	59722.0
44	70323.0
45	80924.0
46	86687.0
47	92450.0
48	98630.0
49	104810.0
50	96918.0
51	89026.0
52	76939.0
53	64852.0
54	54354.5
55	43857.0
56	39419.5
57	34982.0
58	29327.5
59	23673.0
60	21824.5
61	19976.0
62	17319.5
63	14663.0
64	13637.0
65	12611.0
66	10414.0
67	8217.0
68	6802.0
69	5387.0
70	4795.0
71	4203.0
72	3428.5
73	2654.0
74	2131.5
75	1547.0
76	1485.0
77	1203.0
78	921.0
79	707.0
80	493.0
81	305.0
82	117.0
83	85.5
84	54.0
85	50.5
86	47.0
87	29.5
88	12.0
89	10.5
90	9.0
91	7.0
92	5.0
93	4.5
94	4.0
95	2.5
96	1.0
97	1.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1058759.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.48564986572973
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.3080419011319	22.65328947605881
2	10.449023409756578	6.370905382173049
3	3.8546705986886343	3.525364146580335
4	2.066652274599111	2.520129505505697
5	1.2527797751576653	1.90959027921621
6	0.9467285702355414	1.731698142605018
7	0.7093692593568568	1.5137908006385612
8	0.5467197496815628	1.333368549077722
9	0.4489083972607573	1.2316737798609618
>10	4.458135981556574	29.254848445897807
>50	0.7484341308507515	15.82070710962368
>100	0.20272675470171123	8.81177023868854
>500	0.00562262185610654	1.1730108479021932
>1k	0.001874207285368847	1.4258395377203719
>5k	3.123678808948078E-4	0.7240137584510777
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGCT	7603	0.7181048756138082	No Hit
AATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCT	3835	0.36221651952899575	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGT	3669	0.3465377862195268	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCC	3297	0.31140231157421094	No Hit
CCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	1644	0.15527612988413794	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1374	0.12977457570608608	No Hit
ACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTG	1154	0.10899553156100682	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.44502006594513E-5	0.0	0.0	0.8312562160038309	0.0
2	9.44502006594513E-5	0.0	0.0	1.218218688105603	0.0
3	9.44502006594513E-5	0.0	0.0	1.7410949989563254	0.0
4	9.44502006594513E-5	0.0	0.0	3.7934034090855424	0.0
5	9.44502006594513E-5	0.0	0.0	4.089599238353582	0.0
6	9.44502006594513E-5	0.0	0.0	5.64084933398441	0.0
7	9.44502006594513E-5	0.0	0.0	6.801831200490385	0.0
8	9.44502006594513E-5	0.0	0.0	7.767962303035913	0.0
9	9.44502006594513E-5	0.0	0.0	9.361714988963493	0.0
10	9.44502006594513E-5	0.0	0.0	11.45218128015913	0.0
11	9.44502006594513E-5	0.0	0.0	13.525929885838043	0.0
12	9.44502006594513E-5	0.0	0.0	14.414422923441501	0.0
13	9.44502006594513E-5	0.0	0.0	14.786462263839079	0.0
14	9.44502006594513E-5	0.0	0.0	15.166624321493371	0.0
15	9.44502006594513E-5	0.0	0.0	15.496633322597495	0.0
16	9.44502006594513E-5	0.0	0.0	16.192636851256992	0.0
17	9.44502006594513E-5	0.0	0.0	17.211093364967855	0.0
18	9.44502006594513E-5	0.0	0.0	18.4257229454484	0.0
19	9.44502006594513E-5	0.0	0.0	18.989023942181365	0.0
20	9.44502006594513E-5	0.0	0.0	19.589821668576135	0.0
21	9.44502006594513E-5	0.0	0.0	20.177963068082537	0.0
22	9.44502006594513E-5	0.0	0.0	20.88473391961721	0.0
23	9.44502006594513E-5	0.0	0.0	21.55627484630591	0.0
24	9.44502006594513E-5	0.0	0.0	22.080662360367185	0.0
25	9.44502006594513E-5	0.0	0.0	22.570953351990397	0.0
26	9.44502006594513E-5	0.0	0.0	22.99739600796782	0.0
27	9.44502006594513E-5	0.0	0.0	23.480508784340913	0.0
28	9.44502006594513E-5	0.0	0.0	23.91422410576911	0.0
29	9.44502006594513E-5	0.0	0.0	24.397525782543525	0.0
30	9.44502006594513E-5	0.0	0.0	25.074261470268493	0.0
31	1.889004013189026E-4	0.0	0.0	25.519405265976488	0.0
32	1.889004013189026E-4	0.0	0.0	25.95595409342447	0.0
33	1.889004013189026E-4	0.0	0.0	26.37474628314848	0.0
34	1.889004013189026E-4	0.0	0.0	26.809595006984594	0.0
35	2.833506019783539E-4	0.0	0.0	27.23811556737652	0.0
36	2.833506019783539E-4	0.0	0.0	27.67579779723242	0.0
37	2.833506019783539E-4	0.0	0.0	28.098745795785444	0.0
38	2.833506019783539E-4	0.0	0.0	28.520088140927257	0.0
39	2.833506019783539E-4	0.0	0.0	28.962398430615465	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCGAC	30	2.1653395E-6	44.999996	9
TCTAGCG	75	0.0	42.0	1
TACGGGT	250	0.0	39.6	4
CGGGTCT	315	0.0	39.285713	6
CGAGGGC	345	0.0	38.47826	4
TAGGGCC	500	0.0	38.25	5
TACGCAG	65	9.094947E-12	38.076927	1
CCTAGCG	95	0.0	37.894737	1
CCACACG	125	0.0	37.8	1
TTCGCGG	180	0.0	37.5	2
CCGTACT	30	1.13986134E-4	37.499996	44
CACTCCG	60	1.546141E-10	37.499996	1
CGGCTTA	30	1.13986134E-4	37.499996	31
CGCACTG	120	0.0	37.499996	1
TACAGCG	60	1.546141E-10	37.499996	1
GCCGTAC	30	1.13986134E-4	37.499996	43
GCCCCAG	545	0.0	37.15596	1
ACGGGTC	285	0.0	37.105263	5
AGGGCGT	310	0.0	37.01613	6
CGATAGG	155	0.0	36.29032	2
>>END_MODULE