FastQCFastQC Report
Sat 18 Jun 2016
SRR3553132_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553132_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences884788
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGCT37400.42270012703608095TruSeq Adapter, Index 4 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC9360.10578805318336144RNA PCR Primer, Index 4 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAGG1000.042.752
ACTACGG2050.039.5121962
ACGATAG403.4570257E-739.3751
CTTTACG403.4570257E-739.3751
TAGGGCG2250.039.05
AACGGGA7600.038.4868434
ACGTGCG900.037.51
CTATCGG301.1397096E-437.4999962
AGGGCGC1450.037.241386
TACCACG850.037.0588261
AGCGGGA5900.036.9915284
ACAGGGC6050.036.8181844
CAGACGG1650.036.818182
AGGGATC5850.036.538466
TACGGGT2100.036.428574
CGGGATG6350.036.141736
TACTACG751.8189894E-1236.0000041
TTGAGCG250.002106847236.01
CGGATAG504.8767106E-836.01
TACGACG250.002106847236.01
Produced by FastQC (version 0.11.5)