##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553132_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	884788
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.772933177213073	33.0	31.0	34.0	30.0	34.0
2	31.975919655329864	33.0	31.0	34.0	30.0	34.0
3	32.15493315912965	34.0	31.0	34.0	30.0	34.0
4	35.81588244867697	37.0	35.0	37.0	35.0	37.0
5	35.584787542326524	37.0	35.0	37.0	33.0	37.0
6	34.90783441909248	37.0	35.0	37.0	33.0	37.0
7	35.417225369240995	37.0	35.0	37.0	33.0	37.0
8	35.835748224433424	37.0	35.0	37.0	35.0	37.0
9	37.49048472628471	39.0	37.0	39.0	35.0	39.0
10	37.02582652567621	39.0	37.0	39.0	33.0	39.0
11	37.01143211707211	39.0	37.0	39.0	33.0	39.0
12	36.91806285799536	39.0	37.0	39.0	33.0	39.0
13	36.903675230676726	39.0	37.0	39.0	33.0	39.0
14	38.1081061225966	40.0	37.0	41.0	33.0	41.0
15	38.09962951577101	40.0	37.0	41.0	33.0	41.0
16	38.02282015578873	40.0	37.0	41.0	33.0	41.0
17	38.0254852009747	40.0	37.0	41.0	33.0	41.0
18	38.01374453541413	40.0	37.0	41.0	33.0	41.0
19	38.07106109034029	40.0	37.0	41.0	33.0	41.0
20	37.97237756389101	40.0	37.0	41.0	33.0	41.0
21	37.96542787650827	40.0	37.0	41.0	33.0	41.0
22	37.93681537272205	40.0	37.0	41.0	33.0	41.0
23	37.87909420109676	40.0	37.0	41.0	33.0	41.0
24	37.703905342296686	40.0	37.0	41.0	33.0	41.0
25	37.784025099797915	40.0	37.0	41.0	33.0	41.0
26	37.724932978295364	40.0	37.0	41.0	33.0	41.0
27	37.66320406696293	40.0	37.0	41.0	33.0	41.0
28	37.632009023630516	40.0	37.0	41.0	33.0	41.0
29	37.383507687717284	39.0	36.0	41.0	32.0	41.0
30	37.467866878845555	39.0	36.0	41.0	32.0	41.0
31	37.419312874948574	39.0	36.0	41.0	32.0	41.0
32	37.38606875319285	39.0	36.0	41.0	32.0	41.0
33	37.166315546775046	39.0	36.0	41.0	31.0	41.0
34	37.17817940568814	39.0	36.0	41.0	31.0	41.0
35	37.09347549921563	39.0	36.0	41.0	31.0	41.0
36	37.07614479400715	39.0	35.0	41.0	31.0	41.0
37	37.024584420222695	39.0	35.0	41.0	31.0	41.0
38	36.848168148754276	39.0	35.0	41.0	31.0	41.0
39	36.815336555197405	39.0	35.0	41.0	31.0	41.0
40	36.77496304199424	39.0	35.0	41.0	31.0	41.0
41	36.76942160155879	39.0	35.0	40.0	31.0	41.0
42	36.716674502818755	39.0	35.0	40.0	31.0	41.0
43	36.63269619389052	39.0	35.0	40.0	31.0	41.0
44	36.528707441782664	39.0	35.0	40.0	31.0	41.0
45	36.28751633159582	38.0	35.0	40.0	30.0	41.0
46	36.120752089766135	38.0	35.0	40.0	30.0	41.0
47	36.00745941400652	38.0	35.0	40.0	29.0	41.0
48	35.92552227200188	38.0	35.0	40.0	29.0	41.0
49	35.854605849084756	38.0	35.0	40.0	29.0	41.0
50	35.730918592928475	38.0	34.0	40.0	29.0	41.0
51	34.54246214912499	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	14.0
11	7.0
12	6.0
13	14.0
14	27.0
15	38.0
16	74.0
17	141.0
18	269.0
19	446.0
20	850.0
21	1200.0
22	1771.0
23	2338.0
24	3013.0
25	4046.0
26	5077.0
27	6522.0
28	8708.0
29	11335.0
30	14943.0
31	19665.0
32	26123.0
33	35353.0
34	53717.0
35	67575.0
36	84379.0
37	124575.0
38	188634.0
39	223871.0
40	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.395394150915248	31.824120580297198	21.775611784969957	26.00487348381759
2	22.39349991184329	29.332337237846808	21.529112058481807	26.745050791828103
3	20.936879794933926	29.373364014882664	22.11445001514487	27.575306175038538
4	20.030787035990542	30.14417012888963	20.81052184252047	29.01452099259936
5	18.075629416312154	33.38833709317938	19.293435263588567	29.242598226919895
6	20.418563542905193	35.91741750566237	20.213429657725918	23.450589293706514
7	86.23274727957431	3.8642024982255636	5.375524984516065	4.527525237684055
8	88.59353879121326	2.843279972151521	4.121100195753107	4.442081040882109
9	79.55849310795354	6.77970316053111	8.193262114766476	5.468541616748871
10	35.23295975985208	28.88477239745566	14.813492045552154	21.068775797140106
11	24.436701221083467	27.54433830476905	27.58389580328847	20.435064670859006
12	23.6343621296853	22.53217719950994	34.01424974118094	19.81921092962382
13	24.433084535504552	25.036279877213524	28.962644158826745	21.567991428455176
14	20.75954918014259	27.77139834627052	29.22157624199243	22.24747623159446
15	19.7963806019069	31.828980501543874	27.992581273706246	20.382057622842986
16	20.897322296414508	29.415521006161928	27.11214437808831	22.57501231933525
17	19.763491367423608	30.612417889935216	28.859342576978893	20.764748165662283
18	20.23354747125865	29.97045619967721	28.187995316392172	21.608001012671963
19	19.54660325411285	33.32674041691343	27.768233746388965	19.358422582584755
20	21.347938715262867	30.40344127632834	28.360352988512506	19.88826701989629
21	21.04718870509094	30.961767112573856	27.606839152429735	20.38420502990547
22	19.359326753979484	29.703160531110278	28.60798292924407	22.32952978566617
23	19.483763342179145	30.0110308910157	27.283936943086935	23.221268823718226
24	17.95062772099079	31.040430023915334	28.814360050091096	22.19458220500278
25	20.160083545436873	30.542457628268018	27.39232448903014	21.905134337264972
26	21.09635302467936	30.06867181742971	27.526593941147482	21.308381216743445
27	19.81921092962382	29.044358648625433	27.64244090109721	23.493989520653535
28	21.108220274235183	29.97158641392062	27.945903425453327	20.974289886390864
29	19.74642513234809	32.843799870703485	25.736560622431586	21.67321437451683
30	22.406045289945162	30.17638123482687	26.349588828058245	21.06798464716972
31	20.610134857163523	31.88153546386253	24.743780431018504	22.764549247955443
32	21.0662893258046	31.251441023160346	25.620261576784497	22.062008074250556
33	20.8396813700005	30.265668160056418	26.575744698164982	22.3189057717781
34	20.117587489884585	29.446149812158396	28.18087496665868	22.255387731298345
35	20.633191227729128	30.685768794332652	27.174532204324652	21.506507773613567
36	20.675574261857076	31.767722889550942	27.53778306215726	20.018919786434715
37	20.078369055638188	31.363784318955503	25.747297657744	22.81054896766231
38	20.186756601581397	31.477370850418406	27.729128333566912	20.606744214433288
39	18.965786154423434	30.54856078518244	27.825761651378635	22.659891409015493
40	19.991342558895465	30.069689010248783	28.211503772655146	21.72746465820061
41	18.4814893511214	28.47981663404115	30.187796398685336	22.850897616152118
42	20.346342852751167	28.91664443911988	27.84994823618765	22.8870644719413
43	19.333896933502714	28.035190350682875	28.961174880310313	23.669737835504097
44	21.449884040018627	26.81421990352491	27.97438482438731	23.76151123206915
45	21.636369390181603	28.129224175734752	28.48139893398193	21.75300750010172
46	18.930297427180296	28.950211802149216	30.08924171665981	22.030249054010678
47	19.317960912670607	29.566969714779134	28.314127225956952	22.800942146593307
48	19.518913005149255	27.998684430620667	30.419829382857817	22.06257318137226
49	19.98795191616523	27.72246006953078	28.436868492791493	23.852719521512498
50	19.582770109902032	27.547163840377582	28.38058382346958	24.489482226250807
51	20.103346790417593	26.47639886616907	29.996790191548712	23.42346415186463
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1033.0
1	823.5
2	614.0
3	462.0
4	310.0
5	372.0
6	434.0
7	433.5
8	433.0
9	508.0
10	583.0
11	733.0
12	883.0
13	1021.5
14	1160.0
15	1276.5
16	1393.0
17	1955.0
18	2517.0
19	2955.0
20	3393.0
21	4522.5
22	5652.0
23	7080.5
24	8509.0
25	9851.5
26	13818.5
27	16443.0
28	19469.5
29	22496.0
30	25193.0
31	27890.0
32	31034.0
33	34178.0
34	36044.5
35	37911.0
36	40667.5
37	43424.0
38	43614.0
39	43804.0
40	44464.0
41	45124.0
42	47299.0
43	49474.0
44	53146.5
45	56819.0
46	62492.0
47	68165.0
48	72566.5
49	76968.0
50	72547.5
51	68127.0
52	61570.0
53	55013.0
54	48177.5
55	41342.0
56	37346.0
57	33350.0
58	30395.0
59	27440.0
60	26454.5
61	25469.0
62	23404.5
63	21340.0
64	18612.5
65	15885.0
66	13541.0
67	11197.0
68	9924.5
69	8652.0
70	6993.0
71	5334.0
72	4364.0
73	3394.0
74	3317.5
75	2620.5
76	2000.0
77	1563.0
78	1126.0
79	849.0
80	572.0
81	426.0
82	280.0
83	204.0
84	128.0
85	91.5
86	55.0
87	38.0
88	21.0
89	16.0
90	11.0
91	6.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	2.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	884788.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.11989984158056
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.12009843644579	23.807301380267308
2	9.601257526276957	6.167828601944748
3	3.6723980416256614	3.5387117182629857
4	2.0684588453184145	2.6575476375223537
5	1.356309634543822	2.1782264807859146
6	1.0717632273168602	2.065495650918401
7	0.794079064346864	1.7854018009182095
8	0.6654168394118779	1.7098497787848492
9	0.5656914717548617	1.635295807260221
>10	5.35455846243341	36.34084198407501
>50	0.6710246703203286	14.231606451350698
>100	0.055709060559722814	2.8191227005039616
>500	0.0028753063514694714	0.6310124766975564
>1k	3.594132939336839E-4	0.43175753070780487
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGCT	3740	0.42270012703608095	TruSeq Adapter, Index 4 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC	936	0.10578805318336144	RNA PCR Primer, Index 4 (96% over 26bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1302142434119811E-4	0.0	0.0	0.482601481936916	0.0
2	1.1302142434119811E-4	0.0	0.0	0.7363345795829057	0.0
3	1.1302142434119811E-4	0.0	0.0	1.052455503465237	0.0
4	1.1302142434119811E-4	0.0	0.0	2.3184084775109968	0.0
5	1.1302142434119811E-4	0.0	0.0	2.5314538623941556	0.0
6	1.1302142434119811E-4	0.0	0.0	3.7654217733513566	0.0
7	1.1302142434119811E-4	0.0	0.0	4.64585866896929	0.0
8	1.1302142434119811E-4	0.0	0.0	5.497814165653241	0.0
9	1.1302142434119811E-4	0.0	0.0	6.589714146213557	0.0
10	1.1302142434119811E-4	0.0	0.0	8.272716176078337	0.0
11	1.1302142434119811E-4	0.0	0.0	9.72085968616211	0.0
12	1.1302142434119811E-4	0.0	0.0	10.41899302431769	0.0
13	1.1302142434119811E-4	0.0	0.0	10.715674263213335	0.0
14	1.1302142434119811E-4	0.0	0.0	10.978110010533596	0.0
15	1.1302142434119811E-4	0.0	0.0	11.237268136547964	0.0
16	1.1302142434119811E-4	0.0	0.0	11.741456710534049	0.0
17	1.1302142434119811E-4	0.0	0.0	12.46129016216314	0.0
18	1.1302142434119811E-4	0.0	0.0	13.226558226377392	0.0
19	1.1302142434119811E-4	0.0	0.0	13.658639131633793	0.0
20	1.1302142434119811E-4	0.0	0.0	14.06777668774893	0.0
21	1.1302142434119811E-4	0.0	0.0	14.532633806064277	0.0
22	1.1302142434119811E-4	0.0	0.0	15.055583936491	0.0
23	1.1302142434119811E-4	0.0	0.0	15.575708531309195	0.0
24	2.2604284868239623E-4	0.0	0.0	16.001121172529466	0.0
25	2.2604284868239623E-4	0.0	0.0	16.37318770146069	0.0
26	2.2604284868239623E-4	0.0	0.0	16.69959357495807	0.0
27	2.2604284868239623E-4	0.0	0.0	17.07019082537286	0.0
28	2.2604284868239623E-4	0.0	0.0	17.418522855192432	0.0
29	2.2604284868239623E-4	0.0	0.0	17.78154767017636	0.0
30	3.390642730235944E-4	0.0	0.0	18.283701858524303	0.0
31	3.390642730235944E-4	0.0	0.0	18.633616188284652	0.0
32	3.390642730235944E-4	0.0	0.0	18.996867046117263	0.0
33	3.390642730235944E-4	0.0	0.0	19.358309561160414	0.0
34	3.390642730235944E-4	0.0	0.0	19.70212073400634	0.0
35	3.390642730235944E-4	0.0	0.0	20.06548461326329	0.0
36	3.390642730235944E-4	0.0	0.0	20.41268642883945	0.0
37	3.390642730235944E-4	0.0	0.0	20.76248773717546	0.0
38	4.5208569736479245E-4	0.0	0.0	21.138283973109942	0.0
39	4.5208569736479245E-4	0.0	0.0	21.505716623643178	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTAGG	100	0.0	42.75	2
ACTACGG	205	0.0	39.512196	2
ACGATAG	40	3.4570257E-7	39.375	1
CTTTACG	40	3.4570257E-7	39.375	1
TAGGGCG	225	0.0	39.0	5
AACGGGA	760	0.0	38.486843	4
ACGTGCG	90	0.0	37.5	1
CTATCGG	30	1.1397096E-4	37.499996	2
AGGGCGC	145	0.0	37.24138	6
TACCACG	85	0.0	37.058826	1
AGCGGGA	590	0.0	36.991528	4
ACAGGGC	605	0.0	36.818184	4
CAGACGG	165	0.0	36.81818	2
AGGGATC	585	0.0	36.53846	6
TACGGGT	210	0.0	36.42857	4
CGGGATG	635	0.0	36.14173	6
TACTACG	75	1.8189894E-12	36.000004	1
TTGAGCG	25	0.0021068472	36.0	1
CGGATAG	50	4.8767106E-8	36.0	1
TACGACG	25	0.0021068472	36.0	1
>>END_MODULE