FastQCFastQC Report
Sat 18 Jun 2016
SRR3553130_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553130_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806283
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGCT53710.6661432772364047TruSeq Adapter, Index 20 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC11540.14312592476835057TruSeq Adapter, Index 27 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11390.1412655357982247No Hit
AATCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCT8470.10504996384644101No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG551.8189894E-1245.01
CGTCCGC207.032032E-445.015
TAGTCCC207.032032E-445.014
CTAGTCC501.0804797E-940.525
ATAGGGC4450.040.449444
CGCACCG451.9270374E-840.01
CGCCACG451.9270374E-840.01
CCACGCG403.4565164E-739.3751
GGCCGAC356.2459712E-638.5714268
TCTAGCG356.2459712E-638.5714261
TGCACGT356.2459712E-638.57142636
CGGCAGG1650.038.181822
TAGGGCC3950.037.5949365
CGTACTA301.1396196E-437.49999618
CGCCGCA301.1396196E-437.49999618
TATGGGC2700.036.6666684
CTATGGG4000.036.56253
CATAGGG5850.036.538463
GGGCCGA1850.036.4864857
CGAAGGG6250.036.363
Produced by FastQC (version 0.11.5)