##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553130_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	806283
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.659584785987054	31.0	31.0	34.0	30.0	34.0
2	31.866504688800333	33.0	31.0	34.0	30.0	34.0
3	32.053142631061306	34.0	31.0	34.0	30.0	34.0
4	35.72969292419659	37.0	35.0	37.0	33.0	37.0
5	35.499060503570085	37.0	35.0	37.0	33.0	37.0
6	34.81021427960158	37.0	35.0	37.0	32.0	37.0
7	35.353597930255255	37.0	35.0	37.0	33.0	37.0
8	35.775251369556344	37.0	35.0	37.0	35.0	37.0
9	37.445863301098996	39.0	37.0	39.0	35.0	39.0
10	36.920386514412435	39.0	37.0	39.0	33.0	39.0
11	36.897575665120065	39.0	37.0	39.0	33.0	39.0
12	36.85762939315352	39.0	37.0	39.0	33.0	39.0
13	36.82478980705286	39.0	37.0	39.0	33.0	39.0
14	37.97364324933057	40.0	37.0	41.0	33.0	41.0
15	37.962227902609875	40.0	37.0	41.0	33.0	41.0
16	37.87443614710964	40.0	37.0	41.0	33.0	41.0
17	37.88543724721965	40.0	37.0	41.0	33.0	41.0
18	37.86683211725908	40.0	37.0	41.0	33.0	41.0
19	37.93701839180536	40.0	37.0	41.0	33.0	41.0
20	37.828531669401436	40.0	37.0	41.0	33.0	41.0
21	37.833629135179585	40.0	37.0	41.0	33.0	41.0
22	37.807002007979825	40.0	37.0	41.0	33.0	41.0
23	37.73596243502592	39.0	37.0	41.0	33.0	41.0
24	37.57385558172503	39.0	36.0	41.0	32.0	41.0
25	37.64306329167302	39.0	36.0	41.0	33.0	41.0
26	37.602454721233116	39.0	36.0	41.0	33.0	41.0
27	37.52047482087555	39.0	36.0	41.0	32.0	41.0
28	37.511128226689635	39.0	36.0	41.0	32.0	41.0
29	37.30444769392385	39.0	36.0	41.0	32.0	41.0
30	37.32843554930465	39.0	36.0	41.0	32.0	41.0
31	37.35441898192074	39.0	36.0	41.0	32.0	41.0
32	37.31080402290511	39.0	36.0	41.0	32.0	41.0
33	37.1229419447018	39.0	36.0	41.0	31.0	41.0
34	37.17475501777912	39.0	36.0	41.0	31.0	41.0
35	37.11783207633052	39.0	36.0	41.0	31.0	41.0
36	37.08927758615771	39.0	35.0	41.0	31.0	41.0
37	37.00539884879131	39.0	35.0	41.0	31.0	41.0
38	36.87882542481982	39.0	35.0	41.0	31.0	41.0
39	36.867928506492134	39.0	35.0	40.0	31.0	41.0
40	36.80836381270596	39.0	35.0	40.0	31.0	41.0
41	36.78881856618582	39.0	35.0	40.0	31.0	41.0
42	36.68896900964054	39.0	35.0	40.0	31.0	41.0
43	36.604679746441384	39.0	35.0	40.0	30.0	41.0
44	36.51561548488558	39.0	35.0	40.0	30.0	41.0
45	36.345155485108826	38.0	35.0	40.0	30.0	41.0
46	36.22234376763494	38.0	35.0	40.0	30.0	41.0
47	36.128519390834235	38.0	35.0	40.0	30.0	41.0
48	36.02086984346687	38.0	35.0	40.0	30.0	41.0
49	35.91159059536168	38.0	35.0	40.0	29.0	41.0
50	35.824234170880445	38.0	35.0	40.0	29.0	41.0
51	34.68892063952731	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	2.0
10	7.0
11	4.0
12	13.0
13	10.0
14	22.0
15	35.0
16	67.0
17	116.0
18	225.0
19	358.0
20	691.0
21	1070.0
22	1592.0
23	2079.0
24	2765.0
25	3668.0
26	4728.0
27	6208.0
28	8077.0
29	10818.0
30	14199.0
31	18822.0
32	24656.0
33	33774.0
34	50249.0
35	62558.0
36	77356.0
37	112875.0
38	171555.0
39	197631.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.524803325879375	30.923261435500937	22.29911829965409	26.2528169389656
2	21.87569377005344	28.90015044345472	22.43418253888523	26.789973247606607
3	21.04496808192657	29.854033881403925	21.70354577735113	27.397452259318378
4	19.897108087358905	29.867800759782853	20.947483699891972	29.28760745296627
5	17.852788661053253	32.593022549154576	19.797887342285524	29.756301447506644
6	19.611476367478915	36.349643983564086	21.048316782072796	22.990562866884208
7	84.90666428536878	4.043989517328283	6.071069339177435	4.978276858125497
8	87.30358943447895	3.124089184566709	4.575812710921599	4.99650867003273
9	79.18894482458393	6.467456215745589	8.971415743603671	5.372183216066815
10	33.921836377549816	28.572349906918536	15.222570735089292	22.28324298044235
11	23.230429018099105	26.507318150078817	29.285623038064802	20.97662979375728
12	22.26042220907547	21.543304274057622	36.668638678975	19.527634837891906
13	24.157026751153133	24.32359357694507	30.083853932180137	21.435525739721662
14	20.254798873348438	27.594901542014405	30.265923999389795	21.884375585247366
15	19.57017573234212	32.634819288016736	28.340421415309514	19.45458356433163
16	20.226396935071183	29.72380665349511	28.489500584782267	21.560295826651434
17	18.3781625062168	30.33761098770531	30.187167532987797	21.097058973090093
18	18.3249553816712	29.867924785714195	30.108659118448482	21.698460714166117
19	19.235429743650805	32.99970357802409	29.822655320774466	17.942211357550637
20	20.215730704975794	30.47862847164085	29.823151424499834	19.48248939888352
21	19.787841241846845	31.344701550200117	28.431332422982997	20.43612478497004
22	17.827735422922224	30.838179646600512	29.545581390157054	21.78850354032021
23	19.002509044591044	29.852669596159163	28.419673985436877	22.725147373812916
24	17.328779101134465	31.139562659760905	29.175115933239322	22.35654230586531
25	18.694428631138198	30.254761665569035	28.07847864831579	22.972331054976973
26	19.13806938754755	30.672481002327967	28.55089342079642	21.638556189328064
27	16.68049555801127	30.409049924158143	28.872492660765513	24.037961857065074
28	18.356457968232	29.459383367874555	28.59256613372724	23.59159253016621
29	18.78794418336986	30.988871153180707	27.412955500736093	22.81022916271334
30	18.352613164360402	31.395552182050224	27.666712556261263	22.58512209732811
31	17.9495288874998	29.565549565103073	27.301828266253906	25.183093281143222
32	18.932682445245653	29.46422037919688	28.679880389391815	22.92321678616565
33	17.985248355726217	28.362870108882365	29.4984515362472	24.153429999144223
34	16.540470281526463	28.153638362708875	30.50293755418383	24.802953801580834
35	18.258477482472035	27.170608830894366	30.401856420140323	24.16905726649328
36	17.415969330867696	29.14187698363974	31.27028599139508	22.171867694097482
37	18.324583303877173	30.34629280289923	28.69401934556477	22.635104547658823
38	19.16535509244273	28.73953686236718	31.25292236100724	20.842185684182848
39	18.74552731485099	27.32154838933724	32.34447458274576	21.58844971306601
40	19.342960226124077	28.048712424793777	31.015784780281862	21.592542568800287
41	18.75420913004491	27.203971806425287	31.582087182788175	22.459731880741625
42	20.170957343761433	28.162940307559502	28.821145925190038	22.844956423489023
43	17.730002989024946	28.14185589923141	29.608214485484623	24.519926626259018
44	19.59473286674778	26.617949280835635	28.883779020517608	24.903538831898974
45	19.927494440537625	26.551099303842445	31.25354249066395	22.26786376495598
46	17.006187653714637	27.35317500182938	33.254452840999996	22.386184503455983
47	18.721590310102034	27.52048598320937	29.91567476928076	23.842248937407835
48	19.140797958037066	26.07322739038278	32.78017767954924	22.00579697203091
49	19.883217183048632	26.066281938227643	30.348153191869354	23.702347686854367
50	18.295313184080527	25.543512637622275	29.53615541937508	26.625018758922113
51	18.851445460216823	25.41229320226273	32.44530766492658	23.29095367259387
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1441.0
1	1124.0
2	807.0
3	565.5
4	324.0
5	357.5
6	391.0
7	401.0
8	411.0
9	468.0
10	525.0
11	711.5
12	898.0
13	1065.0
14	1232.0
15	1368.5
16	1505.0
17	2056.5
18	2608.0
19	3186.0
20	3764.0
21	4464.0
22	5164.0
23	6494.0
24	7824.0
25	9768.5
26	13585.0
27	15457.0
28	17887.0
29	20317.0
30	23183.0
31	26049.0
32	28458.0
33	30867.0
34	34642.0
35	38417.0
36	39243.0
37	40069.0
38	40670.5
39	41272.0
40	42088.5
41	42905.0
42	43929.5
43	44954.0
44	50506.5
45	56059.0
46	61840.0
47	67621.0
48	72356.5
49	77092.0
50	71031.5
51	64971.0
52	56559.0
53	48147.0
54	41385.5
55	34624.0
56	30644.5
57	26665.0
58	24380.0
59	22095.0
60	20876.5
61	19658.0
62	17268.5
63	14879.0
64	12979.0
65	11079.0
66	9465.0
67	7851.0
68	6699.0
69	5547.0
70	4597.0
71	3647.0
72	3424.5
73	3202.0
74	2497.5
75	1404.5
76	1016.0
77	892.0
78	768.0
79	538.5
80	309.0
81	200.0
82	91.0
83	130.5
84	170.0
85	108.5
86	47.0
87	32.0
88	17.0
89	13.5
90	10.0
91	9.5
92	9.0
93	5.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	806283.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.8226229217585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.14340231122999	24.335809361977397
2	10.390182152281833	6.820660617454634
3	3.799327325934237	3.7411166452641753
4	1.965379838079534	2.5803568529324523
5	1.313574544210649	2.1557480972123457
6	0.9694695986050093	1.909232104147247
7	0.7356694833260583	1.690262143237928
8	0.5809047541731897	1.5253454159746738
9	0.4882005475316389	1.4421620233644314
>10	4.889022052791311	34.26153876922102
>50	0.6467633157498665	14.02000792891164
>100	0.07310233722399608	3.7567190552012715
>500	0.003847491432841897	0.7931945806682528
>1k	7.694982865683793E-4	0.2895709558147275
>5k	3.847491432841897E-4	0.6782754486179248
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGCT	5371	0.6661432772364047	TruSeq Adapter, Index 20 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	1154	0.14312592476835057	TruSeq Adapter, Index 27 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1139	0.1412655357982247	No Hit
AATCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCT	847	0.10504996384644101	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2402593134172493E-4	0.0	0.0	0.7425432509429072	0.0
2	1.2402593134172493E-4	0.0	0.0	1.0847307955147263	0.0
3	1.2402593134172493E-4	0.0	0.0	1.476652738554577	0.0
4	1.2402593134172493E-4	0.0	0.0	3.124213210498051	0.0
5	1.2402593134172493E-4	0.0	0.0	3.358126117008544	0.0
6	1.2402593134172493E-4	0.0	0.0	4.7778509530772695	0.0
7	1.2402593134172493E-4	0.0	0.0	5.797592160568931	0.0
8	1.2402593134172493E-4	0.0	0.0	6.732747682885537	0.0
9	1.2402593134172493E-4	0.0	0.0	8.022369316976793	0.0
10	1.2402593134172493E-4	0.0	0.0	10.04969719068863	0.0
11	1.2402593134172493E-4	0.0	0.0	11.780975166287767	0.0
12	1.2402593134172493E-4	0.0	0.0	12.579454112265793	0.0
13	1.2402593134172493E-4	0.0	0.0	12.901549455960252	0.0
14	1.2402593134172493E-4	0.0	0.0	13.22934999249643	0.0
15	1.2402593134172493E-4	0.0	0.0	13.505059637869085	0.0
16	1.2402593134172493E-4	0.0	0.0	14.157187984863876	0.0
17	1.2402593134172493E-4	0.0	0.0	15.119133108350294	0.0
18	1.2402593134172493E-4	0.0	0.0	16.19170936259353	0.0
19	1.2402593134172493E-4	0.0	0.0	16.717455285551104	0.0
20	1.2402593134172493E-4	0.0	0.0	17.239728482431108	0.0
21	1.2402593134172493E-4	0.0	0.0	17.81533282978805	0.0
22	1.2402593134172493E-4	0.0	0.0	18.469693643546993	0.0
23	1.2402593134172493E-4	0.0	0.0	19.112520045691152	0.0
24	1.2402593134172493E-4	0.0	0.0	19.642358824383003	0.0
25	1.2402593134172493E-4	0.0	0.0	20.09939438137726	0.0
26	1.2402593134172493E-4	0.0	0.0	20.496649439464804	0.0
27	1.2402593134172493E-4	0.0	0.0	20.96224278572164	0.0
28	1.2402593134172493E-4	0.0	0.0	21.38529523752826	0.0
29	1.2402593134172493E-4	0.0	0.0	21.850020402265706	0.0
30	1.2402593134172493E-4	0.0	0.0	22.46159226971175	0.0
31	1.2402593134172493E-4	0.0	0.0	22.904736922395735	0.0
32	1.2402593134172493E-4	0.0	0.0	23.33151015214261	0.0
33	1.2402593134172493E-4	0.0	0.0	23.761880133898394	0.0
34	1.2402593134172493E-4	0.0	0.0	24.16918129242462	0.0
35	1.2402593134172493E-4	0.0	0.0	24.57400193232401	0.0
36	1.2402593134172493E-4	0.0	0.0	24.983163479820362	0.0
37	1.2402593134172493E-4	0.0	0.0	25.39654190898233	0.0
38	1.2402593134172493E-4	0.0	0.0	25.820462542308345	0.0
39	1.2402593134172493E-4	0.0	0.0	26.23768577534191	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATAG	55	1.8189894E-12	45.0	1
CGTCCGC	20	7.032032E-4	45.0	15
TAGTCCC	20	7.032032E-4	45.0	14
CTAGTCC	50	1.0804797E-9	40.5	25
ATAGGGC	445	0.0	40.44944	4
CGCACCG	45	1.9270374E-8	40.0	1
CGCCACG	45	1.9270374E-8	40.0	1
CCACGCG	40	3.4565164E-7	39.375	1
GGCCGAC	35	6.2459712E-6	38.571426	8
TCTAGCG	35	6.2459712E-6	38.571426	1
TGCACGT	35	6.2459712E-6	38.571426	36
CGGCAGG	165	0.0	38.18182	2
TAGGGCC	395	0.0	37.594936	5
CGTACTA	30	1.1396196E-4	37.499996	18
CGCCGCA	30	1.1396196E-4	37.499996	18
TATGGGC	270	0.0	36.666668	4
CTATGGG	400	0.0	36.5625	3
CATAGGG	585	0.0	36.53846	3
GGGCCGA	185	0.0	36.486485	7
CGAAGGG	625	0.0	36.36	3
>>END_MODULE