FastQCFastQC Report
Sat 18 Jun 2016
SRR3553129_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553129_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821025
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGCT57940.705703236807649TruSeq Adapter, Index 20 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC12070.147011357754027TruSeq Adapter, Index 27 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11010.13410066684936514No Hit
AATCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCT9830.1197283882951189No Hit
TACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCT8400.10231113547090527TruSeq Adapter, Index 27 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTG8220.10011875399652873No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA351.211356E-745.0000049
CCACACG351.211356E-745.0000041
CCGATAG253.8898288E-545.01
CGCACCG406.8102963E-945.01
GTACGCG650.041.538461
CCATGCG451.9272193E-840.01
AGGGCGC2100.039.6428576
CGAGATA1250.039.60000219
TGCAACG800.039.3751
ACGTACG356.2461077E-638.571431
TTACGGG4700.038.297873
ACACGAC1000.038.2526
ACAGGGC6600.037.840914
TACGGGT1800.037.54
ACCGGTA601.546141E-1037.49999626
CGCGGCA301.1396379E-437.49999625
ACGGCGC301.1396379E-437.49999616
CTAGGGA5300.037.358494
ATAGGGC4400.037.3295444
TGGGCTC2300.037.1739166
Produced by FastQC (version 0.11.5)