FastQCFastQC Report
Sat 18 Jun 2016
SRR3553127_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553127_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515026
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGCT34050.6611316710224339Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC9220.17902008830622143No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9180.1782434284871055No Hit
AACCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCT5790.1124215088170306No Hit
TACCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCT5390.10465491062587132No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG207.029654E-445.0000041
GCCGGCA207.029654E-445.00000423
CGCTCAG600.045.0000041
CACTTCG207.029654E-445.00000421
ACGCGCG551.8189894E-1244.9999961
CTCACGG1550.042.0967752
TATGGGT1200.041.2500044
TACGCAG603.6379788E-1241.2500041
CGCTCCG451.9246727E-840.01
CTGTACG403.4532604E-739.3750041
ACGGGTC1600.039.3750045
CGGATTA403.4532604E-739.37500440
TAGGGCC1950.039.230775
ACGTAGG750.039.0000042
AGGGCCC1450.038.7931026
TAGCAGG2150.038.7209322
GCCACGG1050.038.571432
CCTAGCG356.2415766E-638.5714261
CCATGCG356.2415766E-638.5714261
GTAGCAT356.2415766E-638.57142629
Produced by FastQC (version 0.11.5)